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mature miRNA: GATGATGCTGCAACAGCAGC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73896505|gb|DT499243.1|DT499243 WS0117.BR_L13 PT-P-FL-A-2 Populus trichocarpa cDNA clone WS0117_L13 5', mRNA sequence

GATGATGCTGCAACAGCAGC
||..|||||||| ||||| |
CTGTTACGACGTGGTCGTAG

EST sequence (targeted fragment marked with yellow):
GACACTTTCTCTTCTCTTTTGCATTCACCCCAGGAAAAGGAAAGACACAATCTTTTCAGTTTTTATGATCCTTCACTTTCTCCCCCATAAAATTAATTAAATCTCTCACTTGCGCTGCTGCTTAATTAACAAGTTTACATTATTAATATTAAATAGAAAAAAAAAAAAACCAAACATCGTCCTCCTAGTTTGTTCACAATGGCGGGCATAGCAATAGTATTAGATCTGTTGTCAAGGAAAAACCCTTCTTCTTATCCAACCCACTTCTCGGCCTCCGCTGCCGCCGCCTCCATCGCCGCCTCCGCCGCCCCTTTTGCTTCTAGGTTCCTGTTCGGGCAACCCATGATACCAGTTGCTTACTGTGATGCTGGTGCAGCATTGTCTGGTGACTATATTTCTAGTATACGAAGAGCATCTGCAGATATTTTTCAGAAAGGCTCTCTGAAATACACTACCAAGGAGTATTATATTGAGTTGAAACCTCTGTTTTCAGCTTTTGAATGGAAGCAACTTGCTATGACATCCTTGAGGTCATTTTTGATGTTCTATTTACCTCTTCTGGAGCCTAGTACTAATA



gi|73884556|gb|DT487294.1|DT487294 WS02533.B21_E23 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02533_E23 3', mRNA sequence

GATGATGCTGCAACAGCAGC
| |||||||||| ||||||
CGACTACGACGTGATCGTCG

EST sequence (targeted fragment marked with yellow):
GACACCCGACTTTTAAAGGTGTTAGGTTACGGAGTGGAAAATGGGTATCCGAGATCCGGGAACCACGTAAAACTACGCGTGTATGGCTCGGTACGTATCCAACTCCTGAGATGGCAGCCACCGCCTATGATGTGGCTGCTCTAGCGCTTAAAGGCACCAACACGCCACTGAATTTCCCGGAATCAATCCTTTCCTATCCAACACCAGCCTCAGCATCCCCTAGTGATATAAGGGCTGCAGCTGCTAGTGCAGCATCAGCCAGGTCACCCAAGCGTGAAAGTGGGCCAAACCCTGAGATGGGCCAGCCTGAAACAGAAGGCTCATCATCTATTATCACTGCTGATGTTCAATCAGGACAGGAAATTTTCGACGAGGAAGAACTTTTAAACATGCCCAATTTGCTAGTTGACATGGCAGGAGGAATGCTAGTAAGCCCACCAAGAATAAACACACTATCTTCTGATGATTCACCAGGGAGTTCCGATGCTGAAAGCCTATGGTCCTACCACTAAAGCAAACCCAAACAGCTTCTCCATGGAGAATTTGCTTCTTGGTTTTGGTCAAAGGGAGAGGTTTTGGTCCCATGATGTAACAAAAGGTTGGCTTTTATTAAAGATTTTTCTATGGGGTAATAGGCAATGGAGTGTTGTGTAGATAAAGGTAAAAACTGGTTGGTGAATCGATTTTGCTGCCACATTTGTTAGTTGTATATATTTAAATGTGTCAGCTAGAGCCAGAGTACGTCTCTCTTTCCATCTGCAAAAAAAAAAAAAAAAAA



gi|73885930|gb|DT488668.1|DT488668 WS02537.B21_B09 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02537_B09 3', mRNA sequence

GATGATGCTGCAACAGCAGC
||||||||||||||||||||
CTACTACGACGTTGTCGTCG

EST sequence (targeted fragment marked with yellow):
TGCTTTCACCTTTGCATTGGCATGATTACAAGAAAAAATATGGAAAGCTAGAGGACTTTGTGGGTGGTCACCCAGAATTGTTTTTGATCGAGGGTGACTTTATCCAGCTTCGGGAAGGAGCTCAAGAAATGATAGCTGCTACAGCAGCTGTTGCTAAAGTTGCTGCTGCTGTTGCAGCATCATCTCCTTACTCATCATTCTTGCCTTCTGTGGCTGTTACTCCCATGGCACAATCTCATCGACTTAAGAAGGTTCCGTCAATTGAATCAAAGTTTTCTAATGGTGTTAATTTTGGGGTTGCTGGAGGCATATCAAATGTAAAATTTTTGAGCAAATCAAAAGATTCTCAAGAACTAAATGTGCCAGATTTTGATAGATCCAGCGTAAGCAGTACTCAAAGCAAGGGCTCAATTCATGGGACATCTAACTCAATTTATACTGGAAAACAGCAGAGCAGGACAACTGGAGCTGCATTAACTTCTAGAAGATAGTATGGAGGCAATGTCCAACATATGAATGAATTCATGGAGTTAACAGGAGTGCAGATTGGGCATTTATGGGAGCGATCTGTGGTAGAATTGTGCTCTTGATGAAATTGCCAGCCATCTTTTGCTTTTGATAGAATCCCCCATTGGACTTAGCTGATTTTTTTTTATCCTCTTTAGTTTTATGCTTTTGGAAAAAACCTGCTTTTTGTGTGTTTATGATACCGAAGACGAACTCGTTCAACTTTCTGCATTGATATTCATGCACACAATTGCTTCTGTGCATTTGATAGTCCATAAAAAAAAAAAAAAAAAAAA



gi|24070221|gb|BU878697.1|BU878697 V050D06 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

GATGATGCTGCAACAGCAGC
|||||.|||||| || ||||
CTACTGCGACGTCGT-GTCG

EST sequence (targeted fragment marked with yellow):
ACTTGCAAAAACCCACTTGGAAAAAATCATCAAACATCAAAAGGGTCTGTCTCATCAGTTCTCAGAAAAAAAATAACAAATGGGTGCTAGTATCACTCTAGGCAGCACCAATTCTTCAGTGTCCCAATCCCATTACCACACTCACAGGGTTTTCTTGTTTTGCAACTACATTCTACTTGGAGCAGCCTCTAGCTGCATCTTCTTAACCCTTTCGCTTCGCCTGGTACCTTCAATATGTGGCTTCTTTCTCATTCTCCTTCATGTCTTCACCATTGCTGGGGCAGTTTCAGGCTGTGCTGCAGCGTCATCCGGGACTAATAGGTGGTATGCTGCACATATGGTGGCCACAGTGTTGACAGCCATATTTCAAGGGTCAGTATCGGTTTTGATTTTTACTAGAACTGGGGATTTCCTAGGGAACTTGAAGTCATACGTGAGGGAGGAGGATGGTGACGTCATATTGAA



gi|73867033|gb|DT469771.1|DT469771 WS01918.C21_N10 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS01918_N10 3', mRNA sequence

GATGATGCTGCAACAGCAGC
||||||||||| |||| .|
GTACTACGACGTAGTCGATG

EST sequence (targeted fragment marked with yellow):
GCTTCCCCATTTCATCGAAAGACTCAAAGGCCATCTATCCCTTTCATTTTATATCAACATGGACTTCTCTTCTTCAAAGAAAACCATGAGAATTTCATTAGCAGTAGCTGATGCAGCATCATGTTACTGTGCTATGGTCCTATTAGGCCTAATATTATTGGGGTCTATTAGGGAGAGTTCTATGACAGAGGAGCCTGTCAAGGGGAGCCACTTTTTTGACAGACCCTGTGATGAAATATATGTTGTCGGAGAAGGAGAGACCCTCAACACCATCAGTGAAAAGTGCGACGACCCGTTTATAGTGGAGCAGAACCCGCATATCCATGACCCAGATGATGTTTATCCTGGACTTGTTATCAAGATCACCAGTTCAAAGTCAAGGAAGATGATGAAGAGGTAGTTCTTGGAGATGAGATACGGCTTCTTGATAGGAATACAGATACCAAATTAATTAGCGTGCTTGTTTTTTTAAAAAACTTCTTACACACATGCTTTAATTTCTATTTAATTTTATTTACTTTGTAATAGGAAAAGAATTATATATATATATTTATACCATAGAGTTTTCAACAAAAAAAAAAAAAAAAAA



gi|73888295|gb|DT491033.1|DT491033 WS02546.C21.1_J24 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02546_J24 3', mRNA sequence

GATGATGCTGCAACAGCAGC
||||||||||||||| |||
CTACTACGACGTTGT-GTCT

EST sequence (targeted fragment marked with yellow):
GGACAATTTGGGAGATGGCCATTGTAGTTTTGGTGGTTGTGAAATACCCATCAAAGAGAGACAAGCGAAGGCTGAGGGCACTGCTGCTGCACGTATGGGTTTAGAGACGCTTCATGGTCTACCAATAGCATCGAGGAACCTTCCAACATGGTCTGGCATGTTCCCTGGTTTAGCATCAAGCTATGAGGAAAGGACTCCGGAGAAGAGGTTTGAAGCAGTTTCTCCACCGAGATTCTCAAGCTGGCCATGGCAGATAAATGGCAGCAACAGTGTTGCTGCTACAATGCCACAGCTCTGTGTTGCAGCATCATCAGGATTCTCTTCTTCGACCAAAACTGTTCCTTCAGCTACTCTTCCATTCAACCAACAGAACCATTTCGCCAACAACAACCCGCGACTTGCAGCCTTCACCACATCTGCCACCAACCCCTCGTTTTACTACTCTTATACGAGCTAATTAACGTTAACACCTTGAGTGCATTTGCCATTGTGCTAAATTAAGCGAGCAAGGGAACTGAATCAATGGAGCCAAAAAA