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mature miRNA: GCCAAAGGAGAATTGCCCTG
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|164394469|gb|DB945966.1|DB945966 DB945966 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_023_D1

GCCAAAGGAGAATTGCCCTG
||||||||||||||||||||
CGGTTTCCTCTTAACGGGAC

EST sequence (targeted fragment marked with yellow):
GGCGTTGGAGTTTGTTGTGATTTTGGTATGCTGCTACTGGGAGACGAAGAGAACTGATAGCGGCCAAAGGGTTAGTAATCCTCTCTCTTCCTAGATGATTGTTCATTTCATTGATAAAATTGCATCGACTCCTCAATACTGCCCTTCAAATTCAACTTCAACTTTTGGGCATATAAGAATCTAGAAATTGCAAAAACCATTTCATTAGAGTTTACTAAAACTTCCATGTAGCTAGGGTTTCCCTAATTAAATATTCCAGCTGTATCTATGATCTGCCTGCGAGTAAGATATCCTGGAAATGTGTCTGCTTCAAGTTGCCTCTTCAACCAGAACCATCAGTGCCTTGGGCAGTTGAACATGGCATGCATTTACGAGACAATTGTGTAATTAGTAGTGTAGTTGAATAACAGGGCAATTCTCCTTTGGCAGCTATCAGTTTAGCAGACTGGTTAACAGCAGAATACCAAATTCCTGCCAAAGGAGAATCGCACTGATATTCTTCACTACCCTCTAGGAGTA



gi|164395169|gb|DB946138.1|DB946138 DB946138 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_023_M2

GCCAAAGGAGAATTGCCCTG
.||||||.||| ||||||..
TGGTTTCTTCTAAACGGGGT

EST sequence (targeted fragment marked with yellow):
TCAAAATCATCTCATTAATTATTATTTCCTTTTTGAGCCTCGAAATTCAGAACTTTTATATTGTGTACGGAACTTCAACGGCCTTTGTAGCATGCAATAATTTGCCCTTCGTTGAAACCCATTAACATTTTTACTCAAATATTTCAGGATTATGCAATGGGATTGGGTTTAGCTTATCAATGTAATAAGATGCACACGTGTGATCAATATATATACACATGCATGAAAAAGAAATCATTCAACTAGAACTTACTAACACTATCATAGAATTCCAAGAGAAGCTGAAAATAAAGGCATTGCTTGAGAGAGAATTGTATGGACTGAATTGTGGGGCAAATCTTCTTTGGTAGTAGGCCTACTCTTTGTAGTTGATTACAACATGAGTTGCCAACTGCCAAAGCAAGAATTGCCCAGGAATGCATACCCATAAACATAAGGGTACGTACCCTTAAACTATGTACTGATGAAACAGTGCCCATAAACATAAGGGTGCGTACCCTTAAACTTTGTACTGATGAAACAGTTTCAAGGTTCAATAGTAATTTATAGTTCAGT



gi|164373532|gb|DB934725.1|DB934725 DB934725 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_041_I0

GCCAAAGGAGAATTGCCCTG
||||||||||||||.| |.
CGGTTTCCTCTTAATGCTAT

EST sequence (targeted fragment marked with yellow):
AATGAACTATCTGCCGATTGTGCACATATTCCAAAAATGAAGGCACATCTGGGTATCGGCAATCTTCTTCACCCGCAAGAGGAGTTTTCTGAAAGCAAATTATGTCTCCATCTTCAAGCTGACTTAATCGAAATGAGGTCCTTTTATCAAGATGTTCACACATGACACAAGGCTCAAACTTTATTTCCTCATAAAGTTCAATCTCTTCATCAGATGCAATAACAGCCATTTGTTTAAGCTTTGCCAAGATTTCCATTGGTTTTCCAGAACCCTTCACAAAAAGCCTACCAACATATCGTAATTCTCCTTTGGCAGGATCATAAAGCTTGAAGAAAAGAAGAATATCTTCTTTAGTTTTTTCAGGAGGAGCAATAGGACGTAAATCCAGTCCAAACTCTACTTCCAAGAACAACTTCAATTCTGCATTGTGTGCTTTATTTGAAACTTCCCTCAGCTGTCCAACTGATTGCGCTTCCTCATGAGCAGTTAGTGGTCGATTGGGACGATATGTATGATTTTGACGCTTTGCCCATATCCAAAATCGCTGAAGTTGTACTGGTACACCAAATTCTTTGGCAACCTCTTCCTTAAAATGACTAAATGATGTCTGCTTCTGGA



gi|56918768|gb|CK643105.1|CK643105 rni.05.F8.5 MBra685 cassava lambda zap Manihot esculenta cDNA 5', mRNA sequence

GCCAAAGGAGAATTGCCCTG
|||||||.|||||||| ||
CGGTTTCTTCTTAACGATAC

EST sequence (targeted fragment marked with yellow):
TTTTTTTTAGAGCAATTATTAGGATGAGGAAAATCAATTACTTTGCAATTGATGTCTGTGCTCCTTATTGTCATCCTAATCTGAAATTTCTACACCTCCATAAATATACAAATGTTATAGAAAAAGATAAAAAAAACTGAACCGATCTTTGAGTCTTGACCTTCACTTCACATGATGAGAGCTGCATACAGGAGTTGATTCCAAGTATCAGGCAATCCAGGAAGGCACCAGTGGCTGCAGTCTGTTCCAGAGCCTTCACCACTATAAGTGGAAGGATGGGCATCTTTTCTTAATTGTGAGAGTGTTGTTATGTCTAGTAGATAAACAGGCTTCTTAATTGAACTTAACACCTTGTTCAACACTGCAGCAGCTGGAGGAGCACCACCTGGATAACTAGAACCTGACAGTGGCTCTGCTTCTCCATAGCAATTCTTCTTTGGCTGCTTCCAATCCCTGCCCTCATAATGGGTTGGAGAAATCCCTTGGAAGAAGACTTTGGTTTTGGAAGCATCAACATTAAGATAAACCCATTTTGCCCATGTTGACATCCCTCTATAAAATGCCTCTAAACGATTCATGTCCTTGTACATTTTTGATCCATCA



gi|56918989|gb|CK643326.1|CK643326 rni.06.J4.5 MBra685 cassava lambda zap Manihot esculenta cDNA 5', mRNA sequence

GCCAAAGGAGAATTGCCCTG
.||||||.||| .|||||||
TGGTTTCTTCTCGACGGGAC

EST sequence (targeted fragment marked with yellow):
CACAACCACAGGAAGATACAAGAGAGAAGAAAAACAGGAAAAGAGCTTGTGAATTGAGCTAGCTAGATAGCCATGGCTAAGGAATTACAGGTTCTTAATGCGCTTGACGTAGCGAAAACACAATGGTACCACTTCACTGCAATTATCATTGCAGGAATGGGCTTTTTCACAGATGCATATGACCTCTTTTGCATATCTCTTGTGACCAAACTTCTCGGTCGCATATACTACCATGTTGATGGTGCAGAGAAGCCTGGAACTTTACCTCCTAACGTATCAGCCGCTGTTAATGGTGTAGCATTTTGTGGAACTCTGGCAGGGCAGCTCTTCTTTGGTTGGCTCGGGGACAAGATGGGGCGGAAGAGAGTCTATGGCATGACACTCATGCTCATGGTGATATGCTCGGTCGCCTCAGGTGTTTCTTTTGGTCACAATGCTAAAGCTGTGATATCAACCCTTTGCTTCTTCCGGTTTTGGCTCGGGTTTGGCATTGGTGGTGACTACCCACTTTCTGCCACAATCATGTCTGAATATGCTAACAAAAAGACTCGAGGAGCCTTCATTGCTGCTGTATTTGCCATGCAGGGCTTTGGAATTTTGGCAGGCGGCTTATTTGCCATAATTATATCATCTGCATTTCGTACCAGGTTTGATGCTCCAGCTTATGAGGTTGATGCAGTTGCCTCTACCGTGCCGCAGGCCGACTACGTTTGG