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mature miRNA: GCCAAGAGAACAAGGTTCCAC
species: Populus tremula x Populus tremuloides
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|60700920|gb|DN490832.1|DN490832 UL91TD09.3pR Populus cold stressed leaves cDNA library Populus tremula x Populus tremuloides

GCCAAGAGAACAAGGTTCCAC
.|||||||||||||.|. ||
TGGTTCTCTTGTTCTAGAGTA

EST sequence (targeted fragment marked with yellow):
GGTATGGATGGGGAAAATGCCTTTTCATTTGGAAAATTGTAATGGTGCTAAACTGTTTTTTTTCGTTTTTTTTTTTCTCTACGCATTGTTTCAAGTCCTCATTACCTGCTGGGAAAAGGATTAACAAATATTGAGGTAGCATTCATCAGATAGTGAATTTGGTTCCCTTGTTTTTTTTAGCTGCTTCTAAAGAATAGTGGTACTTTTCTGTTTTATGGAAATTTTTGCAGGTCCCTTTCAAGGTCAAGATCTCCTCTTGCTTCTCCTCCTCGCAGAAAACATGGCCACAGAACCCCAAGCATAAGCCCCGGTCGTGTTTCCCGTTCGCCTTCTGTGAGGTCTGATCGAAGCCGTTCGGTGGACTCAGATCGTTGATGAGATCTTGTTCTCTTGGTCGTGGAACGTTTCCACTTTCGTAGTGAACTAGACACCTATAAAGCTAGACATGACTCACGTATACA



gi|38584211|gb|CK099886.1|CK099886 A088P10.5pR Hybrid aspen plasmid library Populus tremula x Populus tremuloides cDNA clone A08

GCCAAGAGAACAAGGTTCCAC
.||||||| ||||| || |||
TGGTTCTCGTGTTCAAACGTG

EST sequence (targeted fragment marked with yellow):
TGCCACTCAAAAAACTGTTGATGGTCCATCAATGAAGGACTGGAGAGGTGGAAGGGCTGCTTCCTTCAATATCATTCCAAGCAGCACTGGGGCTGCCAAGGCTGTTGGAAAGGTTCTGCCACACTTAATGGAAAATTGACTGGAATGTCCTTCCGGTTCCTACTGTGGATGTGCTCTGTTGTTGACCTCACCGTCAGGCTTGAGAAGCAAGCAACATACGCAGGCTATCAAATCGGCCATCAAGGAGGAGTCTGAGAATAACCTCAAGGGTATTCTGGGTTACGTTGAAGAGGATGTGGTGTCTACTGACTTCATTGGTGACACGCAGGTCAAGCATATTCGATGCCAACGCTGGAATTGCTTTGAACGATAACTTCCGTGCAAACTTGTGCTCTTGGTATGACAATGAATGGGGGTACAGTTCACGCGTGATTGACT