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mature miRNA: GCCAAGGATGACTTGCCTG
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|164381901|gb|DB944334.1|DB944334 DB944334 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_018_A2

GCCAAGGATGACTTGCCTG
||||||.|||| ||||| |
CGGTTCTTACTTAACGG-C

EST sequence (targeted fragment marked with yellow):
GCTGAGGTGTGGGTTTTACCCCATGGCCAGCAGGTGGCAAGGTCATCCCAAGTGCATGGAACTTCTGAAACTAATGACCAGGTAAATGGAGGCACTTTTTATCACAACTGCAGTGGCTTGCAGACCTCGACACGCCATTCTCACTCAATTGAAAAGAGGGACGAGGGAGACTGTTCAGGCCAACAACGAGGGCGCATTTCATCAAACCAGGCCTCACAGAGGCCTGTTGCTATCCAGTGAACTCTAGTACGCTATGTGGATTGCAATAAGGATTCAATTCACCAATTAGAGACAGATGAATCCTTATATTGGATAATCACATACTTGGCAACGTTTTGCCACACGGCAATTCATTCTTGGCTTTGTTTTGTGTTTGTGACTTAAGATGATGAGAGGGAAACAGGGGATGAAGAGGCAGTTTGTTGGCTCTCTAAGTAGGCCAATGCTCGTCTTTCCTGTGTACATAAACCTGTTTAATAAATAGGAGAAGAAAATAGTCCATGAAGAGATTTTACATGGGGC



gi|164396135|gb|DB946280.1|DB946280 DB946280 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_024_E1

GCCAAGGATGACTTGCCTG
||||||.|||| |||||||
CGGTTCTTACTTAACGGAC

EST sequence (targeted fragment marked with yellow):
CAAATGGGGCCCTCCCCATCAATTAGGTTTCCACCTAAAGTGATGGGCCCCCAACTCTTGCACCGGCAAAGCTGGATGCATCTTGCATACTCAACTTGGGGTTGGCATCCTATTCTATGTAGTGACTTCCTCAGGCAATTCATTCTTGGCTTTGGATACTTTGTAACATGGCGCATTCTCAAAGGTAAAGGAAGACGAAGATCTTCATGGAGTTCCATTTCACTTCCACTCGCCGCTTGTGTAAAAATACTGAAAACTTCAGTTGTGTAGGTATGCATTAGGAATAGTTTGAACGTGTAGTAATTACAGATAAGTTTACTTTTGAGCTACATGCAAGTGTGAAGACTTCTTCTGTTTGTGACAGGAAAAGGGTTACTACAGCTGCCTGCTGAATGTACATTTTAACATTGTTGTTAGTTGGTGTTTGTTTACTGTGTTTTGCTTATTGTTGTGAGATCTCCAACAAGAGCTGGGATTAATGAATGAGAGATTCTACAACT



gi|219215600|gb|FG805107.1|FG805107 K077F01 Cassava root 210-day-old plants cDNA library Manihot esculenta cDNA, mRNA sequence

GCCAAGGATGACTTGCCTG
||||||.|||| ||||| |
CGGTTCTTACTTAACGG-C

EST sequence (targeted fragment marked with yellow):
TCTCGTACTCTGAAGCTAAAACATGTACATGGAGGCACCTTCTACCTCAAACACAATGGCTTACAAACCTTGACACATCACTCACTTGCAGGGGAAAAGGGGGAGGAGGGAGACTGTTCAGGCCAACAACGGGGGAGCATTTCCTCAAATCAGGCCTCACAGAGGCCGCTTTCTATCCAGTGAACTCCAGTATGCTAAGTGGACACCAAGATGGAGGATTGGATTCACCCTAGCAGAGACAGCTGAATCCTCCTAATTTTTGGTGACCACATCCCTGGCAAAATGTTGCCAGGCGGCAATTCATTCTTGGCTTGGTTTCATGTTTACGACTCTAATGACGAGAGCGAGACGAGGGATGGGATGAACAGGTGTTGGCTTCTTTTAAGTCGGCCAATCCTCTACCATCTTTTTCCCATTTTCACTTTTCATGTGTATAGTAACATGTGGAATAAATGCGAGTAGAAATAGTCTGTGTGGAGAGCTTCTAAATGGGGAAACAGCTCTTTTAAGTTTAGCATAGGAACTGTGTTTGTCCGACTGACATGGGTTTATTTGCGTGCAATTGGACGGTGGACATTTTACTGCCTCTCTCCATTGATGGTGTATATTTACC



gi|164394889|gb|DB945970.1|DB945970 DB945970 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_023_D2

GCCAAGGATGACTTGCCTG
|||||||||||||| || .
CGGTTCCTACTGAAGGGTT

EST sequence (targeted fragment marked with yellow):
CTTCCACCCCTTCTTGCTCAGACATCACAAGTGCATCCAACAGAGACGAAACTTTCCAGCAGCCAGAATTCATGTTCTCTGGGTACCATTCTCACATTGTGGGGACCATGCAAGGGCGCTGAGTCGGTACGCGGAGGCATACAACAAATCTCGTACGCTCCTTGTTCGGCAACAATCTGAAGAGTTGCTTATGTTCCCTTGGGAAGTCATCCTTGGCTCAGTTAGTCTGCATTTAGTTTGTTTCATTGCATGCAAGCTTATTTTGGAATAGTAGTGATCTGACTAGACGCATCTGTATTTTGTGGTTGCATGTACTTTTGTTCTAACACTTGCACTAATGTATGGGTGGAGCTCTGCATATTTGTCCATTTCATCTTTGGTTCTGCAAGTTCTCGCTCAACTTTTCTCCACTTTTAGCTAAAGTGATGAAGTACCTTTATGTGCCATTGCAAAATGATGCCAAGTTTTGTTTGAT



gi|164376945|gb|DB953908.1|DB953908 DB953908 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_048_A2

GCCAAGGATGACTTGCCTG
||||||.|||| |||||..
CGGTTCTTACTAAACGGGT

EST sequence (targeted fragment marked with yellow):
GCAGCAGCAGATAACTGCTGCAACCTGAAAGTTTAATGGCAAAGGGTTTGATTGGGCTGAGTCCCAAATTCCATCCCCTAAGCATACAAATGAAAAGGTCTGTTTTGTTGGCAGAGCTTTGTGTGGAGATTGACTCCTACATTTGGTTGCTGGGCAAATCATTCTTGGCTCATATGCATTAAGCTCATCTCTATCATTCCATGAAATTCTGAATTCACTGGATGACCAGAGATGAATACCTTAATGGATACTTATAAAAAAAATTAAGGATTAGGACTAGAAAATAAAGTGGGTCCATCCTCATCCCTCTTGTAATAATTATGTGAAAGTCTGGTTGTGTGGCTGTGATCTTACATTATGTTGAAGCATGATCTGTCTGGCTTGTATTATCACCTATATTTCTGTAATCACCATGTGTTGTCC



gi|119018022|gb|DV454709.1|DV454709 CV02016B1H05.f1 CV02-normalized library Manihot esculenta cDNA clone CV02016B1H05.f1, mRNA s

GCCAAGGATGACTTGCCTG
|||||||||||||| || .
CGGTTCCTACTGAAGGGTT

EST sequence (targeted fragment marked with yellow):
TCCGATAAGGCAACAGGCTCTGCCGAGCTTCATTCGGCTGGAAATATGTCAGAATCTGAAGTTCATCAGCCAGAGAATCACAGAGATGGTGCTTCCACCCCTTCTTGCTCAGACATCACAAGTGCATCCAACAGCGACGACATCTTCCAGCAGCCAGAATTCATGTTCTCTGGGTACCATTCTCACATTGTGGGGACCATGCAAGGGCGCTGAGTCGGTACGCGGAGGCATACAACAAATCTCGTACGCTCCTTGTTCGGCAACAATCTGAAGAGTTGCTTATGTTCCCTTGGGAAGTCATCCTTGGCTTAGTTAGTCTGCATTTAGTTTGTTTCATTGCATGCAAGCTTATTTTGGAATAGTAGTGATCTGACTAGACGCATCTGTATTTTGTGGTTGCATGTACTTTTGTTCTAACACTTGCACTAATGTATGGGTGGAGCTCTGCATATTTGTCCATTTCAAAAAAAAAAAAAAAAAATGC



gi|164394457|gb|DB945954.1|DB945954 DB945954 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_023_C2

GCCAAGGATGACTTGCCTG
||||||||||| |||||.|
CGGTTCCTACTTAACGGGC

EST sequence (targeted fragment marked with yellow):
CTAACGATGAATCCATTTTTAGGCAGTCAGATCACCGTTTCGCAAGCCTAGCTGCACATATCGGTGGTAGCATCCAAAGCAGTGGGGGTCTTATGTGCAGTGGAACTTAGAACTATGCTTCCAGTTGTCCGGATACCATTCCGGGCGGGCAATTCATCCTTGGCTTTGTCTATTTCTTGCTATTATAAGCTATCACGTTTTGTTATGAAATAAGCTTCTGACTTAACTGTGGTGTTTGCTTTTACGAGTCTCATGTTTGTGTTTAACATTACTGAAAAAAGTTTGCTTTTGGGATTTTGCATTGTGGGTGTTCATCCATTACTTAGCTTACTTCATGTGATGGCCAGAGATGTGAGGTTTG



gi|119010381|gb|DV448363.1|DV448363 CV01024B1A11.f1 CV01-normalized library Manihot esculenta cDNA clone CV01024B1A11.f1, mRNA s

GCCAAGGATGACTTGCCTG
|||||||||||||| || |
CGGTTCCTACTGAAGGGTC

EST sequence (targeted fragment marked with yellow):
GTCACTNNNNNATTTCTGAAGCCATATCTTCATGAATCTCGGCACCTTCATGCACTGAGGAGGGCTAGGGGTTCTGGTGGACGCTTTCTCAATGCGAAACAGTTCCAGGAGTCCAATCCTAAACTCTCAAGCCTGGATGCCACAGGCACTGCTGAACTTCATTTGGCCGGAAATATGTCAGAATCTGAAATTCATCAGCCAGAAAATCACAGAGATTGTGCTTCTACCACTTCTTGCTCAGATATCACAAGTGCCTCCAACAGTGATGACATCTTCCAGCAGCCAGAGTTCAAGTTCTCTGGGTACCCTTCCCATGTGGTTGGGACCATGCAAGGGCGCTTAGTTGGCATGCATGGCGGTGGAAGTCAGCACCATCTTCCTGTTCTGTGAGAGAAGAGGCTACGACCAACCTCAGCAGTATGTGTTTGTCACAGCTGCTAATGTTCCCTGGGAAGTCATCCTTGGCTCAGTTAATCTGCATTTTGTTTTGTCTCATTGCATGCAAGCTTATTCTCAAAAACTTATGATGTGATGTGATGTGTGTGTTCATTTTGTGGTTGCAGATACATGTGAATTGAAAACTTTATGGCAACTGATGTCAAATTTAAGATAATGTTTCATTTTGCTCAAAAAAAAAAAAAAAAAATCG