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mature miRNA: GCCAAGTATCTTGGTTTTGCA
species: Lactuca sativa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|83983091|gb|DW129200.1|DW129200 CLSX5986.b1_C10.ab1 CLS(XYZ) lettuce sativa Lactuca sativa cDNA clone CLSX5986, mRNA sequence

GCCAAGTATCTTGGTTTTGCA
.||||..||||| ..||||||
TGGTTTGTAGAAATGAAACGT

EST sequence (targeted fragment marked with yellow):
CACAAGAATACGTTTTTTCGTTTTCAATTTCTCTGGAACCATGAATCGCTCGTCAAATGTGTTGATCTTTATTTCTTTCTTCTTTTTATCATTCACCGGAAATTTAAATCTCGTTGACGCTGGTGATACAGCCTCAGTTATATTGAATTCAGGAAATTTTGAACAATTGGTTATGCAAAGTAAAGATGTTTGGTTTGTCTTGTTTGGAGCACCCTGGTGTCCATATGTTAAACAACTTAAACCTGAGTGGGACAAGGCTTCAAAGGCTTTGGAAGGAAAGGTCAAGTTTGGTGAACTTGACTGTGATGTGGAAAATGATATACAAGCAAGGTTTGGAGTAACTGGGTTCCCGACTGTTTTTGCATTTGGTCCAGATAAATGTGCACCTCCCAGCGGACTAACCGAAAGAACTGCAGACGCACTTGAAAAGGCTGGCCTGTCTTACTATGACAAAGCACAGGGGTGTAAAATGGAGTTTTGAGTCGAGGATTGTATTCGAATAAGATTTGTTTGACTTGTTATCGATATGAAACAATAATGGCTCCTTTAACCCTGGTGTAAAGAGCTAATAAAGAGTAATTTTAATTTTGATCTCCGAAACCAAAGCCGAAGCTCCTGCATGTGATCCTCGGGACTCAAATTGTGTGGTAAACCCCTCCAGAAAGAACCCGCTTGTCATAGAAGGGGGTATAGATAATAAGGAGGCGGAAAAATAAAAAGAAGATGACACCCCTGAGATAATATAGTTGGTTACAGGAAGATGTCACGTTGA