miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: GCTCTTCCTCTTCCTCATCAT
species: Lolium perenne
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|300182940|gb|GR517625.1|GR517625 ELPLPE1034F11-b1FSP_20010122 Perennial ryegrass etiolated seedlings library (LPE) Lolium per

GCTCTTCCTCTTCCTCATCAT
|||.|||||||| ||||| |
GGAGGAGGAGAAGAAGTAGGA

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGACAAATTAACTATCTCTTATTTTCAGAAGGCAATTTACGACCAATTTCTTCTTACACCTACGTACAGTTCAAGGTTGTCACGTAACACAACAAAGAAAGCACACCGGACGTACAATTAGAGAGTGGCAAAAACGACCTCGTGCTCATGATACATATACATCGATCTAGTCGTCGTCCTCGCCGCTGATGAACTTGCCGGTTCCTGGGTTGATGGCGGCGATGAAGAAGAAGACGACGTTGAAGAGCACGAGCGGCTCGATCTCGGCGATGGGCACACCGGTCTCGATGTTGAGCTGCGCCAGCGCGCCCTTCCCGGTGATGATCTCACCGATGATGGAGAAGGCGATACCCAGCTGCGCCAGCCGCCCCACGAACAGCTCGTTCGACTTGGTGAACCCAAAGAGCGGCCCTCCCTCGCTGAGGCCGAGGGCAGAGCGGAAGCCTTGGCCTGGGGCGATGACGGCCTTGTCAAGGCCGGTGGGCTGCTCATCGACGAACCTTCCACGGTCACCGAGGGCACCGATAGCGCCTAGGAGGGTGAAGAGGATGAAGAAGAGGAGGAGGGGCTCCGCCTCGTAAATCGGGATACCGGTCTCAAGGTTCAGCTGGGACAGAATGCCCTTGCCGGTGATAGCCTCTCCCAATATCGATGCGGCAAAGCCAATCATGGCAACACG



gi|300192778|gb|GR519781.1|GR519781 ELPLPE1035H03-g1RSP_20001212 Perennial ryegrass etiolated seedlings library (LPE) Lolium per

GCTCTTCCTCTTCCTCATCAT
|||||.|||||| | ||||||
CGAGAGGGAGAAAG-GTAGTA

EST sequence (targeted fragment marked with yellow):
CCCAAATTCCAAATTCCAAATTCCAGCGGATTCTGAGACTGGAGCTTGTGTCGGGGCATTCGATTCCAGAGCCGGTGACGGAGGGGGATGTCAGGACCCCTCGACCGATTGGCCAGGCCATCTTTCGAAGGGTTCACACATAATGATGGAAAGAGGGAGAGCAGATCGGACGCGGACAACTCAGAGGGAGAGAAGAAGACTAAAATAAACTCTTTCAAGAAAAAAGCTATCAATGCAGGGAACAAGTTCAGGCATTCCCTAAGGAGGAAAAACAAAAAGAAGAATGAGATTTCCATCGAGGACATAAGAGATGTACAAGATCTTCAAGCTGTTGATGCGTTTCGACAGTGCTTGCTTGATGAGGACTTGTTGCCACAGCAGCATGATGATTACCACATGATGTTGAGGTTCCTGAAGGCACGCAAGTTTGATGTTGAAAAAGCAAAGCATATGTGGTCAGAAATGCTTAAATGGAGAAAAGAATTTGGGACTGATGATATAGAGGAGTCTAATTACTCCGAGTTAAACGAAGTTATGAAGTATTATCCACAGTTTTACCACGGGGTGGATAAAGACGGAAGACCTATCTACGTGGAGTTAATAGGAC



gi|300185818|gb|GR510408.1|GR510408 ELPLPC1003D07-g1RSP_20010824 Perennial ryegrass 3 wk seedlings, cold-acclim. for 7 days at 4

GCTCTTCCTCTTCCTCATCAT
|||| ||||||||||| |||
CGAGTAGGAGAAGGAGCAGTT

EST sequence (targeted fragment marked with yellow):
GGAGGCGGGGGCGCCCGGGCCTCGCCAGCTTCGCGCCATGCGCGCTCCCGCACGCCTCGGGCTTATCGCTTCACGGCCGCTGGGGCAGGCCCAAGACCAGGCATAGTCACATATTGCGTGCTGCTGGACCTGACGAGCCACATGTTGCAACCCCAACATGGTCTGAGGCAGCTCTCGACAAACCTTATCTCGATGAAGCGATCGGAAACGAGGAGCTTGAAGCTGTCCTGGACACACCTCTTCCTTCCCATCCTAAGCTTATCCGGGGCCAGTTGAAGAATGGCCTCAGATATCTTATTTTGCCAAACAAAGTTCCAGCTGATAGGTTCGAGGCTCACATGGAAGTTCATGTTGGATCAATTGACGAGGAAGAGGATGAGCAGGGAATTGCACATATGATTGAGCATGTTGCATTCCTTGGGAGTAAAAAGCGCGAGAGACTCTTGGGGACAGGTGCAAGGTCTAATGCATATACAGACTTCCACCATACAGTCTTCCATATACATTCTCCAACTAAAACAAAGGAATACGGTGAAGCCTTACTCCCTTCTGTGCTGGATGCTTTGAATGAGATAGCTTTTCATCCAAAGTTTTCTTCATCTCGTGTCGAGAAAGAGAGAAGAGCAATTCTGTCAGAG



gi|152039954|gb|ES700407.1|ES700407 ve_008b_b03 Lolium perenne ve Lolium perenne cDNA, mRNA sequence

GCTCTTCCTCTTCCTCATCAT
||||||||||||||| || |
GGAGAAGGAGAAGGAGGAGGA

EST sequence (targeted fragment marked with yellow):
GGAAGCCGCCGGAGATAGGGGATACGTTGGTTGAGAGGGCGGCTGTGGTCGCCAAGGACTGGACAGAGCGGTTTGGGATCAAGATGGACACGCCCGCACCCTGTAGCCAGGAGGTGGAGATGACAGAAGCGGCTGTCGTAGATAATGTAGCAGCGGCAGAGAAGAAAGTAGAGCCTGTAGATGTGAAAGAGGAGGATGAGCAGGAGGAGGAGGAAGAGGAAGAGGAAGAAGAAGAAGTAGAGGAAGAGGAAGAGGAAGAAGAAGAAGTAGAGGAAGTAGAGGAAGAAGAAGAGGAAGACCCGGAGGAGGTGGTGGCTGCATCAGGCGATGAGGAAGAAGAGAATCCCGAGGAAGTAGACAATGAAGCACAGGAGGATCCAGAGGAAGCGGAGGAGAAGGGCCAAGAGGTGAAGGTGGAAGTGGCTGATGAGGAGAAGAAAAAAGAACAAGCAGAAAAGAGCAAAGTTGAGGAAGTGAAGAAGGCAGCTGGAGGAGTCAAGGAGGAGGAGAAGAGTGCACTGGGGCAAGCAGAGGCTCATATATTCCTGCAGATGGTTGCGGCATTTGCCTTGAAAGACAAGTTCAACCAAGAGTTCTTGAGGAGCCTGTTTCT



gi|300187651|gb|GR512990.1|GR512990 ELPLPF4001A03-b1FSP_20010626 Perennial ryegrass seed immature, 7 weeks library (LPF4) Lolium

GCTCTTCCTCTTCCTCATCAT
|||||| ||||||||||| .
GGAGAAGCAGAAGGAGTAGGG

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTGAGCAAATAACAATAATACTCTTCGAAAGTTCAGAAGTACAGACCCAAATGTACAACACCCCCCAGTCCATGTGTCGATGGCCATAACACAGGCACATCCCAACGCAAATAAAGCGAACTTGCAGCTACAGAGCAAGCACACTAACAATAACGCAGCTCAACATCGAACAATTGGTGAGTACGAATGACGATCTCAGTCGGCCTTCTTGGAGACGTTCGATTTGGCGGCGCCGTCATCGTCCACGGACGACATATCCCTCGAACGCTTGTCCTCGGCCGTCTTGGCGTAGACCTCGTCGCGTGTGTGGAAGTAGGTCTCGAACGCGCGGGGGAGGAACTTGGGGATGAGGAAGACGAAGAGGTTGATGGAGAAGTAGACGATGTTCGCCACGGCGAGGTACCTCCCGAACCAGACCCACGCCTTGTCCTGCACGGGGGCGGTGCGGGTGACGAGCTTGTTGGTCCACACGTCGTAGACCCAGTCGACGATGACGAAGACGCGGCGCACGGTGTAGAGCAGCGGCACCAGCGCCCTCAGCGGCGGCGAGAAGATGCCGAGCGAGCCGATGATGTTCTCCGTCAGGATCTGGCAGGAGAGCAGGAACAGGTGCGGCGTCGCGGACCGCACCGCGGCGTCGTCGCCGCGCGCGAACCCG



gi|152039258|gb|ES699711.1|ES699711 rg2_002_h05 Lolium perenne rg2 Lolium perenne cDNA, mRNA sequence

GCTCTTCCTCTTCCTCATCAT
||||||||||||||| |||.
GGAGAAGGAGAAGGAGAAGTG

EST sequence (targeted fragment marked with yellow):
GGGAAAAAGAGGTCTTATCTGTTTGCTTGTGATCAAATCGAAGATTTAGAGCCAAGCCGAAAGTACCAAAGGAGCACTATTCAGACCGTAGTTGGAGGAGAAATTACTTCAAACTGCCCATCAATCCTAGAAGAGAAGTGTTCGAAAAATGTACATGATGATCTAGCATTTGCAAATGGTAAAGTTGATGAGTCAGATTTTGCTGATGATGATGAACCTTCAGAGGATATCACAGTGTCTGCAACTGAACCATCTAATGGATTGCTTGATAAGCAAGAGGACGATGCTAACGGTGAAGAGGAAGAGGAAGAGGAAGAGGAAGATGAAGATGAAGAGGAAGAAGAGAAAATATCGCCAGGGAAGAAGTTGTGGCGATTTCTCATCACATGAGTAGGTTCCACTTATGATAGAGATACAGAGCTGACTGTAGGCTGACAGTTACCTCTGATGAAGATATTTTCCTTTAGGTCCATTCATCTGCATCTCCACCTTTCGTATTGAACGGTGGCACGTTGTTGGGTACTTGTTGGTTGTGTGCCTGTTATTACCAGCGGTGGTGGTTTTCGAAGCAGCTGTGGAACTACGTGATCTATTTTGTGTTTTCACATCCCGGGCACTTCAACATCTAAAGGGTTGATAGCTTCCTGGATGTGTACCAAATGATTGGGGGT



gi|152039258|gb|ES699711.1|ES699711 rg2_002_h05 Lolium perenne rg2 Lolium perenne cDNA, mRNA sequence

GCTCTTCCTCTTCCTCATCAT
||||||||||||||| || |
GGAGAAGGAGAAGGAGAAGGA

EST sequence (targeted fragment marked with yellow):
GGGAAAAAGAGGTCTTATCTGTTTGCTTGTGATCAAATCGAAGATTTAGAGCCAAGCCGAAAGTACCAAAGGAGCACTATTCAGACCGTAGTTGGAGGAGAAATTACTTCAAACTGCCCATCAATCCTAGAAGAGAAGTGTTCGAAAAATGTACATGATGATCTAGCATTTGCAAATGGTAAAGTTGATGAGTCAGATTTTGCTGATGATGATGAACCTTCAGAGGATATCACAGTGTCTGCAACTGAACCATCTAATGGATTGCTTGATAAGCAAGAGGACGATGCTAACGGTGAAGAGGAAGAGGAAGAGGAAGAGGAAGATGAAGATGAAGAGGAAGAAGAGAAAATATCGCCAGGGAAGAAGTTGTGGCGATTTCTCATCACATGAGTAGGTTCCACTTATGATAGAGATACAGAGCTGACTGTAGGCTGACAGTTACCTCTGATGAAGATATTTTCCTTTAGGTCCATTCATCTGCATCTCCACCTTTCGTATTGAACGGTGGCACGTTGTTGGGTACTTGTTGGTTGTGTGCCTGTTATTACCAGCGGTGGTGGTTTTCGAAGCAGCTGTGGAACTACGTGATCTATTTTGTGTTTTCACATCCCGGGCACTTCAACATCTAAAGGGTTGATAGCTTCCTGGATGTGTACCAAATGATTGGGGGT



gi|300183643|gb|GR514954.1|GR514954 ELPLPH1008A02-g1RSP_20010524 Perennial ryegrass 3 wk seedlings, heat-stressed for 8 hrs at 3

GCTCTTCCTCTTCCTCATCAT
||||.|||||||| || || |
CGAGGAGGAGAAGCAGGAGCA

EST sequence (targeted fragment marked with yellow):
ATCAGATCACGATCTGGAACTGAAGTCGATCTCCTGTGAACCTACCACAGAGCAGGAGCTGGAGCAGGATGAGGAGGAGGAGGACGACGACGAAGAAGAAGAGGAAGAGGATGAACAGGACGAGGACGAAGAGGAGGAGCTAGCTCCAGAATCCTCCATGGAAGAGGAGAAAAACGACTTCGCTCACCACCGTAAGGACTGGGAATTCACATTTAAGCACGGGAATCGTACCTTCGATCTTATCACGTCACTGAGTCCAATGGTCTACACACACTGCACGCCGAGCTGCCTCCCAGAAGATGCCGATCTCGTCCCAAGCCTACAGATGTTCTCCATCAAAATCGCCAAGATAAAGCACCTCCAGTGGCCGCTTCATGTCTACGGCACTGTCGCTGCCCGGGACTACGTGGATCACAAGCGGAACATCATCTTCTTCCGTCCGAGGAGTGAGAGCCAAACCATCACCAAAAATGATCCTTATTTGCACCTGACCGGACCGCACCGCGGGCTCCTGTCCGTCGAGCCTGTACACATTGAAATCCGACTCATGCTAAAGGAGGGACAGTCTTGGCCTGAAGATA