miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: GCTCTTCCTCTTCCTCCTCTA
species: Aquilegia formosa x Aquilegia pubescens
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|75453978|gb|DT764424.1|DT764424 EST1198273 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1Z245,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||| |||
GGAGGAGGAGAAGGAGAAGAA

EST sequence (targeted fragment marked with yellow):
TCAAAGAGAGTGATGAAGGATTCAAGTCAGGAAGTGAGGTGGGTGAGGTGAGGGCATGAGGCAACTTGTTCATCAAGAAATCAACCAAGCAAGAATGTTATTTATGGTTCAATAAATAATCAAGACTTTACCACCACCACCACCGCCGCCGTCTTGGTTGTTTTCTCTCACCACCACACTAGTACGGGCAGATTTGGTTTAGCTTTTTGCAAGCTATCATCTCTGCTTTATTCTTTTAGTTTCTCTCACAATGGCGGTGGACCCAGTCAAAGAACAGTTGAATCCTAAACCTGTTGAAGAAGTCCCAATTGAAGAAGAGTTGAGCGGAGATAACAAGGACCATGACAACTTGGATGAAGATGAAGAAGAAGAAGAGGAAGAGGAGGAGGAGTTAAGTGGAAGATCAAGATTGATTTCTGAGAGAATCAAAATGAACAACTTCATGGAAAGATTATCTAAAGAAAATGTAGACCTTCGCGTTCATGACATTATCATCAAGGGAAACACTAAAACCAAAGACTCGTTCATTGAAGCTGAGGTTGGAGTCCTCAAGAAAGCCACCACTATGCAAGAGCTTATTCAAGCTACTAGTATTATTAATGCTCGTCTTCAGAGTCTTGAAATTTTCGATGGAATTAGCATTACCATTGATGCTGGCCCACCTGAGCTTCCAGGAACTGCAAATGTCATTGTCAAGGTTGTGGAGGCTAAAAGCACCCTCGTTGGAAACTTTGGCATCTACACCAAGCCTCAAGCTAGATCAGGGAGTCTTGAAGGGTCAGTCAAGTTGAGAAACTTGCTTGGATATGCTGACATATGGGATGGTTCGTGGGCTTATGGGTTGGGATGAAGCTTCAGAAATCAGCGCTGGGATACATTTACCGAGGTTC



gi|75462326|gb|DT769541.1|DT769541 EST1203391 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZX75,

GCTCTTCCTCTTCCTCCTCTA
|||| |||||||||||||.
GGAGATGGAGAAGGAGGAGGA

EST sequence (targeted fragment marked with yellow):
GAATTTCTCTACTTATATAGACTTCACTCTTCTCTCTTTAGCCTCCTTCATCATCATCACAACTCCTTTCCCCCTCTCTTTGTTTCTGAAAACCCTAATTTCATTTAACCCTAATTTCTTCCCTTTTTTCTTCAATAATGAGACCTCCAGCAAGAGGTGGTAGTCGTGGTGGGTTTAGAGGAAGAAGTGATGGTGGTAGAGGAGGAGGAAGAGGTAGAGGTGGTGGTGGTAGAGGAGGATGGGATGGTGGTAGTGCTGGTAGAAGTGGTGGAAGAAGTGGTGGTCGTGGTGGA



gi|74536325|gb|DT743554.1|DT743554 EST1177403 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1VK91,

GCTCTTCCTCTTCCTCCTCTA
|||.|||||||| ||||||
GGAGGAGGAGAAGAAGGAGAA

EST sequence (targeted fragment marked with yellow):
ATCGAAGAAGAAGAAGAAGAACGATGGAAGGAATAGCAGAAAGACTATCAACAAAAGAGAATCTTTACTTTCCAAGAGCAATTCAAGAAATTCTTGCTATAGATCCTTCCAGAACAAAATCCATTCTTCTTGATCTTCTTACTAGAGATACTGCTGTCTTCTTAGAGAGATATGGAGCAGAATTAACAAGTGAAGAGTTAAAAGAATTTGAAATATTGAAGAAAGATGAAGATGATTATGAAGTGAATTGGCATTTAAAAAATTTGAGAAGTTTAATTAATCCAAGTTTAGAAGAATTGAGATTACAATCTATTAAGATTAAGAATAGAAGAAGAGCTTATTTGAATAAACTTGTTAATGATAGGAATTATTTTTCAGAAGATGCAATGAGAGAAAGAGAACCTTATTTACATCATGAATTGTTAGGGAAGTTTCAGGATCCTAGTGGTAGAAGTATGGCGAGACCAGGTGAGAAATGGTCAGAGACTTTAATGAGAAGGTCTGAAGAAGCGTTTTTAGTTGAGAAGATTAGAGGAGAACAGAAGAGGTTAGGTGTTGATGAGAGAGATTGGGTTGGTAATGTGAGAGAAGAACAGCAAGAAGAAGAAGAGGAAGAAGAGGAGGAGGAGGAAGATGATGATGATGATGATGATGAAGGAGATGACAAAGAAAAGATGCAGGTGGACGGCAGCGTTGAGATCATACTGAGGTGGTAGGTACCGAGG



gi|71717543|gb|DR948180.1|DR948180 EST1139719 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1R960,

GCTCTTCCTCTTCCTCCTCTA
||||||||||||| |||.|
GTAGAAGGAGAAGGAAGAGGT

EST sequence (targeted fragment marked with yellow):
ATCAATCATGGCAGAGAAAGAAAGCAACAGCAGTAACAACAGTGATGGAGAAGGAAGAGGAAGATGGTCTCTTAATGGCATGACTGCACTCGTCACTGGTGGAACCAGAGGCATTGGGCATGCAATTGTAGAAGAACTATGTGGACTAGGATCAATAGTACATACTTGTTCTAGAAATGAATCTGAACTTAATGACCGTTTACAAGAATGGAAGCTTAAGGGTTTTCAAGTTACTGGTTCTGTTTGCGATGCGTCTTCTTCAACAGATAGAGAGAAGCTTATTGAGACTGTTTCTTCTCTGTTCAATTCCAAACTCAATATCCTCGTAAATAATGTGGGAACAAATATAAGGAAACCAACTGTGGAGTATTCTTCGCAAGAATTTTCTACCCTTATTGCTACCAATTTGGAATCTACTTATCATTTGTGTCAACTTGCGCACCCACTTTTGAAAGTGTCTGGAATGGGAAGCATTGTGTTCATTTCTTCTGTTGCTGGTGTAGTGGCAATCAGTTCTGGTACTGTTTATGCTGCTAGTAAAGGAGCTATCAATCAAATTACTAAGAATTTGGCGTGTGAGTGGGCCAAGGACAACATTAGGAGCAATTGTGTTGCGCCATGGTATATTAAAACCTCACTCGTAGAAAAGTTGCTTGATGACAAAGAATTTGTGGAAAGGGTTGTCACAAGGACTCCACTTCGACGTGTTGGAGAACCTAAAGAGGTTTCAGCGTTGGTCGCATTTCTTTGCTTACCTGCTTCTTCTTACATTACTGGGCAGATTATTTCAGTTGATGGTGGGATGACAGTGAATG



gi|71716444|gb|DR947081.1|DR947081 EST1138620 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1R306,

GCTCTTCCTCTTCCTCCTCTA
.||||||||||||||| || |
TGAGAAGGAGAAGGAGAAG-T

EST sequence (targeted fragment marked with yellow):
ATTTCTCTCTAATTAGGGTTTCGCGCTTCAATCAATTTTGATTTGAATTAAAGTTGTAGAGAAGAAGAAGAAGAAGAAAGATGGGAGAAAGAAAAGTATTGAATAAGTATTATCCACCGGATTTTGATCCAGCGAAACTTCCTCGAAGAAGACAACCTAAAAATCAACAAATTAAAGTGCGTATGATGCTTCCTATGAGTATTAGATGTAATACTTGTGGAAATTATATCTATAAAGGAACTAAGTTCAATTCGCGGAAAGAAGATGTTATCGGCGAGACATACTTGGGAATTCAAATATTTAGGTTTTATTTTAAATGCACAAAATGCTCAGCTGAACTGACGATGAAAACGGACCCACAAAATTCAGACTATGTGGTAGAGTCTGGGGCGTCCCGCAATTATGAGCCTTGGCGTGCCGAGGATGAGGAAGCAGATAAAGAAAAAAGGAAACGGGACAGTGAAGAAATGGGGGACGCGATGAAATCTCTGGAGAATAGAACACTAGATTCAAAAAGAGAAATGGACATACTTGCTGCTCTAGATGAAATGAAATCCATGAAGTCTAGACATGCTGGTGTCAGTTCAGAGGAGTTGCTTGAGGCCTTGCAACGTACAGCTGAAGAGAAGGAGGAAAAGTTGGAGGCAGAGGATGAAGCTCTGATAAAAGCAATTTATCAGGGTCCAAGAGCTATTATCCGAAGGATTGAAGATGATGATATTGATGAAGATGAAGATGAAGATGAAGAGGAAGAGGAAGAGTTACATATTCCACTGACAATGGTGAA



gi|74546584|gb|DT750268.1|DT750268 EST1184117 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1WP53,

GCTCTTCCTCTTCCTCCTCTA
.||||||||||| |||||||
TGAGAAGGAGAACGAGGAGAA

EST sequence (targeted fragment marked with yellow):
GTTATTCAGCTAGTGATATGGAATCAGATGAGAGTGGGAGTGAGAGTGAGAGTGAGGATTCTTCTTCGTCAAGTGAGAAGGAGAAAAGACGTAGTAAGAAGTCAACAAGTAGACGGAGTAAGCGTGGGAGAAAGAGGAGCAAGAGGAAGAGTAAGAAAGCTAGGAAGACTGTTGGTTCTGATGAAAGTTGTAGTAAAAGTGATAAATATGATTCAGATGGATCTTCTGATGACGATGCAAGAAGTAAGAAACGGAAAGGTTCTAGCTTGGATTCTGATTCCATCAGGAGCAAGAAAAAGAAACAATCTGGATCGGCTAGTGAGAGTTCAGGCAAAGGCTTGTCTGGTTCTGATACTGATGCAAAAAACATTACTTTGAGTGATGACTTAATGAAAACTGAGATGAACAGTGAGGCCCTTGAATTTATAGAGTTGCTAGAACTTCAAAAGAAACCCGACTTAGACGAAGAAGCTGTTGTGGGACCTATGCCTCTGCCGAGAGCTGAAGGACATATAAGTTATGGAGGAGCTCTACGACCTGGTGAAGGTGATGCTATTGCTCAGTATGTACAACAGGGGAAACGTATTCCTCGAAGAGGAGAAGTGGGTCTTTCAGCTGATGAGATTCAGAGGTATGAGGACCTTGGGTATGTAATGAGTGGTAGTAGGCATCAAAGGATGAATGCCATTCGTATTAGAAAGGAAAACCAGGTGTACAGTGCTGAAGACAAGAGAGCATTAGCTATGTTTAACTATGAAGAGAAAGCGAAACGTGAGCACAATGTCATGGCTGATTTGCAGAGGTTGGTACAGCGCCATATTGGGGGAGGAT



gi|74549689|gb|DT752450.1|DT752450 EST1186299 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1X248,

GCTCTTCCTCTTCCTCCTCTA
|||.|||||||| ||||||
GGAGGAGGAGAAGAAGGAGAA

EST sequence (targeted fragment marked with yellow):
AATCGAAGAAGAATAAGAAGAACGATGGAAGGAATAGCAGAAAGACTATCAACAAAAGAGAATCTTTACTTTCCAAGAGCAATTCAAGAAATTCTTGCTATAGATCCTTCTAGAACAAAATCCATTCTTCTTGATCTTCTCACTAGAGATGCTGCTGTCTTCTTAGAGAGATATGGAGCAGAATTAACAAGTGAAGAGTTAAAAGAATTTGAAATATTGAAGAAAGATGAAGATGATTATGAAGTGAATTGGCATTTAAAAAATTTGAGAAGTTTAATTAATCCAAGTTTAGAAGAATTGAGATTACAATCTATTAAGATTAAGAATAGAAGAAGAGCTTATTTGAATAAACTTGTTAATGATAGGAATTATTTTTCAGAAGATGCAATGAGAGAAAGAGAACCTTATTTACATCATGAATTGTTAGGGAAGTTTCAGGATCCTAGTGGTAGAAGTATGGCGAGACCAGGTGAGAAATGGTCAGAGACTTTAATGAGAAGGTCTGAAGAAGCGTTTTTAGTTGAGAAGATTAGAGGAGAACAGAAGAGGTTAGGTGTTGATGAGAGAGATTGGGTTGGTAATGTGAGAGAAGAACAGCAAGAAGAAGAAGAGGAAGAAGAGGAGGAGGAGGAAGAAGATGATGATGATGATGAAGGAGATGACAAAGAAAAGATGCAGGTGGACGGCAGCGTTGAGAATCATACTGAGGTGGTAGATACCGAGGCTTGCAATGTTGCAAAGTCTCATCAGAGAATAACTCTGTCACCTGCAGAGATGCAAGATCACATTGAACAATTCACTCATATCATGCAGCAGAAGT



gi|74545694|gb|DT749629.1|DT749629 EST1183478 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1WL66,

GCTCTTCCTCTTCCTCCTCTA
.||||| |||||| ||||||
TGAGAATGAGAAGAAGGAGAA

EST sequence (targeted fragment marked with yellow):
GAAAAAACTCTCCAAGAAATCTTCTGAGGTGAAAAAGAGGAAGAAGAGTAAGAGTAGAAAAAGCGATAAATCTAAGAAACTTCGTCGTCACAGGGATGTTTCTATTTCATGTTCAGATGATGATTCCAGGAGTGAGGATTCATTATCGTCTGATTCGGAAGATGGGTATAGACACAGAAAGGGTAGGTCTGGTACTCGAGGGGATGTTAAAGGGAGTAAGAAGAGGGTGAAGAGAAGTTTGTCTAGTTCTGGAGACAGTCGAGATCCACCCAGTCGTAAGAAAAGGAAAAGGTCCAAACGAAGTGATGATTCTAAGTTGAAGAAGAAGTCTCTCATGGGAAGTCTAAGAAGAAACGATCCAAGAGAGATGTGAGTGTTGATTCTATTAGTAGTGATTCAAGGAGTTGCTCAACTAGTCATGGAGGGAGTAGCGGTGAGAAAATTCAGATTAAGAGGTCAAGGGGAAGGTCTAAAGAAAGGGATAAGGGTAAGAAAAGTTTGGATCAGATAAGTAATGGAAGAGACAGGTATAATTATAGGTCCAGGAGTTGCACTCCTTGCAGCGGAGCCAGTGAGCGAAATCATTATTATAGTGAGGAAAAATTGGTGAGTGAGAATTACTCGAAAGGTCTTAGATCAGTTCTTACTATTCCAGATGAATATGAGGAAGGAATATATCAGAGTAAGGATGGGAATAAACCAGAAATTATTCAGGCTTATGATGATTGTCCATCATGTAAAAGCAATGACAGTTATGATGGGGGGAGAACGAAAGAGTCTTCTCACCAGGCACAAGCTTTGCTTGAAAAGAAGAGACGGG



gi|71705845|gb|DR936482.1|DR936482 EST1128021 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1PB94,

GCTCTTCCTCTTCCTCCTCTA
.|||||||||||| || |||
TGAGAAGGAGAAGAAGAAGAA

EST sequence (targeted fragment marked with yellow):
CTCTAGCTAGTTACGTACTCATCGTCTGTTATATATCAATCAACAAAATGCCTGGTTTTCAATTTGTTTTTCCTTCTCCGTTATCGCTTTATCAACAATGGCTTTTACATTGTGAGAAAGAAAAACAAGTGGAAGAAAGAAACAAGAAACAAAATAATACTACTACTACTACTACTAATCATCGTTCACCCAAGTTGCATAATTTTGATATACTGATGAAGAATGACAACAACAACAACTCATCAACTGATCAACAACCCAAGTTGCATCTATTTAATATACTCAAGAAGAACACTTCCGTTGAAAAAGAGATTAACTCATCTCATGAGTTTGGTTGCTTTACTTCCAAGACGACAGAAAGTAAACAAGAACCTGTTTCTGCAGTGATGACCCAAGAACAAGAAGAAGAAGAGTTATTAAAGCTGAGATTAGGAGTCAATGTTGGGGTTGTTCCTATTGATCATCACAAAGTAGTTTCTGGAAAACCTGTTTCTGCAGTGATGAACCAAGAAGAAGAAGAGGAAGAGTTATTAAAGCTGAGATTAGGAGTCAATGGTGGGGTTCCTATTGATCATCAGGAAGTAGTTTCTGGAAAAGGGGTTCGTTTGTTTGGGGTTTTTTTGATGGAGAATGATAAAATCAATACCACAACCAAAACAATCTCAACAACAACAATGAAACCCTCCTCTGCTGCTGCTGATACTCTGCAGCAGCCTTTAATGCTTGTGAAAGAGAAGCACACTTATTGTTGTTGGTAATACGCA



gi|71711115|gb|DR941752.1|DR941752 EST1133291 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1Q693,

GCTCTTCCTCTTCCTCCTCTA
.||||| |||||| |||| ||
TGAGAACGAGAAGTAGGAAAT

EST sequence (targeted fragment marked with yellow):
ACCCATACCTAGCTCAGAGAGCCTGCCAAAAAGAGGAAACCTCAGCAAGCATCCACTCTCAAGCAACATCAGAGGCAAAGGTTAAGATTGAGATCAAACCAGAACTTAGTGATGAGAGTGAACAAACATCGACTTCTCAATGGAAGTCCCTTCGAACCTTGACCACTACAGTTACTAGAAAAACTGATAAGAAGAGGAAAGGCACACTCAAGAAAGGGGCAACCAAGAAACGCAAATGTTCTCAACCAGACAGCCCAATTGAAGCTGTAGAAGACTCACCTACACATGATTCCCCTCCACGGTGTAGGAGAATTCTCAGAACTGGACAGGCAAACCAGAAGACATTGCCTATTTCCAAGAAACGTAAATGTTCTCAACCAGACAGCCCAATTGAAGCTGTAGAAGATTCACCTACACATGATTCCCCTCCGCGGTTTAGGAGAAACCTCAGAACTGGACAGGCGAAGCGGAAAGAGGAGGCTGCTGGTGGACGCGGCAAACGCCTGAAGAAAAAGTCTTATAAGCTCTTGGAAGATGAAGATGTTAAACCAATAAAACAAGCCAAGAAGAAAATTACGAAGAAGGCTTCAACCTGCAGCGAGGACAAAATAATAAAGGATGAAGAGCAAGAGTACCAGTGCGATATAGAAAACTGTACAATGGTTTTCAGATCGAAGCAAGATTTAAAGTTGCATAAGCGCAACATTTGTTTGGTTGAGGTTGCGATAAGAAGTTCTTATCTCACAGTATCTGGAGCAGCATGAGCGAGTCCACATGGATGATCGGCCCCTAAAGTGCCC



gi|71708679|gb|DR939316.1|DR939316 EST1130855 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1PS65,

GCTCTTCCTCTTCCTCCTCTA
|||| |||||||||||||.
GGAGATGGAGAAGGAGGAGGA

EST sequence (targeted fragment marked with yellow):
CTCCTTTCTCCCTCTCTTTGTTTCTGAAAACCCTAATTTCATTTAACCCTAATTTCTTCCCTTTTTTCTTCAATAATGAGACCTCCAGCAAGAGGTGGTAGTCGTGGTGGGTTTAGAGGAAGAAGTGATGGTGGTAGAGGAGGAGGAAGAGGTAGAGGTGGTGGTGGTAGAGGAGGATGGGATGGTG



gi|74530250|gb|DT739840.1|DT739840 EST1173689 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1UQ37,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||| |||
GGAGGAGGAGAAGGAGAAGAA

EST sequence (targeted fragment marked with yellow):
CGTCTCCAACACACTGCAGTACTGCACTGCGTCTCTCTTACTATATCTATCTTATCTTGCTGCAATAAAATAAAATCATTGATTCTGCTTAAAACCTTTTTTTAGTTTAAGGGTTTAAGCAAACAACTCTTACAATAAACCTTGGAGAAAATTAAATAGAGCCCACCACCACCACCACCACTGCCGCCGTCTTGGTTGTTTTCTCTCACCACCACACTAGTACCGAACAAACAGATTTGGTTTAGCTTTTTGCAAGCTATCATCTCTGCTCTATTCTTTTAGTTTCTCTCACAATGGCGGACCCAGTCAAAGAACAGTTGAATCCTAAACCTGTTGAAGATGTCCCAATTGAAGAAGAGTTGAGCGGAGATAACAAGGAGCATGACAACTTGGATGAAGATGAAGAAGAAGAGGAAGAGGAGGAGGAGGAGGAGTTAAGTGAAAGATCAAGATTGATTTCTGAGAGAATCAAAATGAACAACTTCATGGAAAGATTATCTAAAGAAAATGTAGACCTTCGCGTTCATGACATTACCATCAAGGGAAACACTAAAACCAAAGACTCGCTCATTGAAGCTGAGGTTGAAGTCCTCAAGAAAGCCACCACTATGCAAGAACTTATTCAAGCTACTAGTATTGTTAATGTTCGTCTTAAGAGTCTTGAAATCTTTGATGGCATTAGCATTACCATCGATGCTGGCCCACCTGAGCTTCCAGGAACTGCAAATGTCATTGTTAAGGTTGTTGAGGCTAAAAGCACCCTCGTTGGAAACTTTGGCATCTACACCAAGCCTCAAGCTAGATCAGGAGTCTTGAAGGGTCAGTTAAGTTGAGAAACTTGCTTGGATATGCTGACATATGGGATGGTTCGTGGGCATACGGTTGGGATCAGCTTCAGAAATCAGCGCTGGTATACATTTGCCG



gi|75455716|gb|DT765578.1|DT765578 EST1199427 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1Z958,

GCTCTTCCTCTTCCTCCTCTA
|||||| ||||||||||||
GGAGAAGAAGAAGGAGGAGAA

EST sequence (targeted fragment marked with yellow):
TCACACATTGTAGGCTTGTAGCCAGTATAACATATAAGCATGTTGCTAAGAAGTTCATCAACTCCAGTTCTTGGATCCCTTTTCTCATCTTGGTCAGATACTCCTAACAGAGATTTTGATAACAATAGTCATAACAACAAACACTCATCATCCACCCCTGATCACCATCACAAGAAAATATCATTTTTACATGGTGGCCATTCCAATTTCACTTCTTACTCTTGCAATTCATCTCCTATCAGTACTCCTTCCAATAATAAGAGCATAGGTGGTTTCAGAAGAGCTCAATCAGATGGGAACTTAGAAGGCTTAATTGCTGCCTGTGATACAGACGAGTTTCATTCTTCCAAACCTCTTTCGCGATCTTTGCGTAAACCTCATCATTCAATGTTGCAGACTATTCCTTCTTTTTCAATATATAACTCAACAAATGAGTTTGAGGAAGAGGAGGAAGAAGAAGAGGAAGGATGGGGTGGTGATTTTAGTGACAATGATGGATTTTTGAAGAGGTCTATTACCATTGGGGAGAATATTAATGGAGTAGGAAGTGGGAATTTCAGTTTTGGAAAGAATATGAATTTGATAGTGGAGGAAGAAGGTGTGACTGAGTTTCATAACTTTAGTAATGATGAAGAAGGCGAGCCTAAGAGTCCACCCCTCTACTTGGCAAGAGGACTTGGTCTTGATGTGGGCTTTGGTG



gi|75462361|gb|DT769565.1|DT769565 EST1203415 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZX92,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||| |||
GGAGGAGGAGAAGGAGAAGAA

EST sequence (targeted fragment marked with yellow):
GAGCAAACATGGCGTCTGAGCTTGTCCTTGCAGCAACAAATGAGAAACTGCAGGAAATGGATTGGATGAAAAATATTGAAATCTGTGAACTAGTTTCACAAGATCCAGGGCAAGCTAAAGATGTAATAAAAGCTGTTAAAAAACGAATGGGGAATAAACATGCAAATACTCAGCTTTGGGCCATTAATTTGTTGGAGATGCTGATGAATAATTGCGGAGAACATATACATAGGCAGGTCATTGATTCGGGACTTTTACCAGTTCTAGTAAAGATAGTCAAGAAAAAGTCGGAATTGCCTGTGCGTGAGAAGATTTTTCTTCTTCTAGATGCAACACAAACATCCCTTGGTGGAGCTTCGGGAAAGTTTCCCCAATATTATGCTGCATATTATGAGTTGGTGAGTGCAGGAGTAAAGTTTTCTCAAGCACCTCGTGTTGTCCCCTTGGAAGTTCGTACCACAGAAATGCATAAAAGCAATACACCTGGGGTGAAACCCGCGTCTCCAAAAAGTGTGGAGTCCACTCAGAAACCAAAGTCTCAGATTTTCCCCGAGTCCAGTATAATTGAGAAAGCTGGAGCTGCTTTGGAGGTTTTGAGAGATGTGCTGGATGCAATTGATAGCCAGAATCCTGAGGGAGCAAAAGATGAATTCACGCTGGATCTTGTGGAACAATGTTCATTTCAAAAGCAACGTGTAATGCATCTTGTGTTAACATCTCGGAATGAGAATGTGGTTTCTCAAGCAATTAAATTGAACGAGCAGCTGCAAAAAGCGTTAGCTAGACATGATGCTCTTCTTTCTGTTAGGGCTACTTCTACTGCCACTCAATTTGTCCATGAAGAAGAGGAAGAGGAGGAGGATGAGCT



gi|75460030|gb|DT768170.1|DT768170 EST1202019 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZP60,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||| |||
GGAGGAGGAGAAGGAGAAGAA

EST sequence (targeted fragment marked with yellow):
TCGCGAAAAACGTTTTGAGTTTCTTCTCTCTCTCTCTCTCTCTCACCTTCGCTTCAACTCTTCGATTTCGAACAAAGCTCTAACTTTCCTCTCTGTTGCTTTGCAAGCCTACTTTCTTAGAAACAGAAGATGCCAAGAGGAAGGGGGAGTCGGAAGGACGTTGAACCAGAAAAGCCTACTGAGCCCGAGAATCCAGTTGAACCCGAAGAACAGGTGGACCTTGATGGGGACAACGATGCTGAGGAGCAGATGGAAGAAGAAGTTGAGTATGAAGAAGTAGAGGAAGAAGTAGAGGAGGAAGAGGAGGAAGAAGAGGAAGAGGAGGAGGAAGAGGAGGAGGAAGAGGAAGCTAATGGAGTTGATGAACAGGCAAGCCCTGATGACGATCAAGATATGAAGACTTCAAATGTTGATGACGAAGAGAGCAAAAAACATGCTGAGCTTCTTGCGTTACCATCTCATGGTTCTGAGGTGTATGTTGGTGGCATTCCACATGATGCTTCGGAGGAGGATTTGAAGGGTTTTTGTGAGTCAGTAGGAGAAGTCACTGAGGTTAGGATGATGAAAGGCAAAGATTCGCGGGAGAACAAGGGTTATGCATTTGTAACATATAGGACTAAGGATTTGGCTGCCAAGGCCATCAAAGATCTGAATGGTACTGAATTTCAGGGCAGAAAGATAAAATGTTCAACCTCTCAAGCGAAGCATAAGTTGTTCATTGGTAATATTCCTAGAAGTTGGACAGAGGACGATTTACAGAAAATAGTGGATGATGTTGGACCCGGAGTTAATGGCGTGGAGTTGCTGAAGGATCCTCAAAACTCCAACCGTAATCGTGGTTTTGCTTTCATCGAGTACTATAACC



gi|74535268|gb|DT742977.1|DT742977 EST1176826 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1VH45,

GCTCTTCCTCTTCCTCCTCTA
|||||| |||| ||||||.|
GGAGAAGAAGAAAGAGGAGGT

EST sequence (targeted fragment marked with yellow):
TACTAATCTAAGGCCTCTTGGGCTGCATCAACTTCTAATCCATGAACATTGTCATTTTTATCTTCATCCTTTTCTTCTGCACCTTCTTCTTCTTCTTCTCCATCTTTTTCTCCTGCATTTTCTTCTTCACGTTGTGTTGATTGTTTTTCGGGGCGAACTGGATGCAAACGTAGGCGTCCATCTTCCCTGTATGCATTGAAGAACACGTGAGTGGGAATTCTGCACTGTCTAAGAATAAACCTTCCATCACGTCGAGAAGATCTGAAAAACAGCCAAGGCTTGCCATTCCCACCTATGCTAGAAATTGGAGGAGGAAAGCTCTTGAGTTTTCTTAGTTTTCTATACTCAGCTTGATGCTCCAATGATGCATTCTTCCTCAAACTTGCCTGTCTGTGATATTCTTTTGGAACATCATCTACACTCTCATTCCTATCGACATCAAAGCTTTCAAATCCAAGTCCCTCCGTGCAAAGCTGCAAATTAACGTTCAAGGAGGAGGATTCATCGTCAGAAGGTGCGCTCTTAGTTCTACTTATGTTGGTTTCGTGGTGGTTCTCATTGCCGTCGCTCTTGGTTCTTCTTATGCTGTAGATATTGTCGTCATCATTGGAACTTTCGGTTTTGCTTCGTCGATTCACATTTAGTGGAGGAGAAAGAAGAAGAGGGGGTGGTTCTTTAAAGCAAAGTTCGCCAAATAACTCTATAAAAGAAGAAGCATCAGGAGGTTTAGGTGGTGCAACTTGGTGATTGTTATGGCGATTGATATCGTGTTCATCATTGAAAGTTTGAATCTGAGACTGTGAAGAAGAACTAGCATCATCCTCATCATGTTGACAAGGACAATGATCTTTGAA



gi|74562744|gb|DT760212.1|DT760212 EST1194061 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1YD34,

GCTCTTCCTCTTCCTCCTCTA
||| |||||||||||| ||.|
CGAAAAGGAGAAGGAG-AGGT

EST sequence (targeted fragment marked with yellow):
AGATACACAAGAAGAATCTGTTGCCCAAGTGCTCAAAGCTGGATTGGATCTGGACTGCGGAATGTACTATACAAATTTCACAAAGAATGCGGTGGTGAAAGGGAAAGTGAGAGAGAGGGATATTGATAATGCTCTAAGGAACCTATACGTTGTACTTATGCGGCTTGGATTCTTTGATGGTAGCCCTTTATTCGAATCATTACACAAAGATGATATATGCTCTGAAGAACACATTGAATTAGCGGCAGAGGCAGCTAGAGAAGGAATTGTTCTTCTTAAGAACGATAACTACTCATTACCTTTGAGACCGGAGCTATATAAATACAAAACCATTGCTATAATTGGTCCTCATGCTAATGCGACCGATGCCATGATCGGTAACTATGCAGGAGTTCCTTGTAGATACACAAGTCCTCTGGATGGATTCAATGGCACTACCAATGTGATATTTGAACCTGGATGTCTTGACATTGCCTGCAAGAATGAGACACATATCTTCAAAGCAATGCAAGCTGCAAAGAAAGCAGATGCTACTATAATTTTTGCAGGGATAGACTTGTCAGTGGAGGCAGAGAGCTTGGACCGTGTTAACCTGTCACTCCCTGGATACCAAGAACAATTGATTAATCAAACTGCCATGGCTGCCAAAGGTCCTGTTATTCTTGTTATCCTCAGTGCTGGAGGTATCGATATTGGTTTTGCCAAGGGTAATCCTAGTATCTCATCTATAATTTGGGCTGGATATCCTGGAGAGGAAGAGGAAAAGCCATTGCAGATGTTATTTATGGAAACTA



gi|71707651|gb|DR938288.1|DR938288 EST1129827 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1PM49,

GCTCTTCCTCTTCCTCCTCTA
|||| |||||||||||||.
GGAGATGGAGAAGGAGGAGGA

EST sequence (targeted fragment marked with yellow):
CTTATATAGACTTCACTCTTCTCTCTTTAGCCTCCTTCATCATCATCACAACTCCTCTCTCCCTCTCTTTGTTTCTGAAAACCCTAATTTCATTTAACCCTAATTTCTTCCCTTTTTTCTTCAATAATGAGACCTCCAGCAAGAGGTGGTAGTCGTGGTGGGTTTAGAGGAAGAAGTGATGGTGGTAGAGGAGGAGGAAGAGGTAGAGGTGGTGGTGGTAGAGGAGGATGGGA



gi|74547745|gb|DT751045.1|DT751045 EST1184894 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1WU22,

GCTCTTCCTCTTCCTCCTCTA
.|||||| ||| ||||||||
TGAGAAGTAGATGGAGGAGAA

EST sequence (targeted fragment marked with yellow):
CATTAACCCTGGTGAAGCGTATTAAAGAAATAAATTCGAAAGAAGCAGCATTAGGGATTTCAGAAGATGCTTCATGGCATGCTAAGTATAAAGATTCAGCTTATGTTTTTGTTGGTGGATTACCTTATGATCTTTCTGAAGGTGATCTTCTTGCTATTTTTTCACAATATGGTGAAATTGTTGATGTCAATCTTGTTAGAGATAAAGCTACTGGTAAATCAAAGGGTTATGCATTTGTTGCTTATGAAGATCAAAGAAGCACAAATCTTGCTGTTGATAATTTGAATGGAGCTAATGTTGTTGGTCGAACTATAAGGGTTGATCATGTTAGTAATTATAAAAAGAAAGAGGAGGTAGATGAAGAGTTGGAGCAGCAAAAAAGGGAGGAACGAGGTGTTTGTCGTGCTTTTCAAAGAGGGGATTGCACCCGTGGAGTGTCTTGCAAATTTAGCCATAATGAAGAGAGAGCGGCAAATACTGGCTGGGGGCACAAAGATGACAGTTCTACATGGGAACACGACAAGCATGAGAACCCACCAATAAAGAGTGGCAGAAATGGTGCCAAACCCTTGGATCGTATTCCTGAGTCCCGAGGTCAGAAAGGAGCTCTTAATCTCAGATCAGATGATAGGGGTAAGAGATTTTCAAGACAAGATCATGATAATGCAGGCAGAGAAGACTACCCCAGGAGAAGTGAGCCATATCACGA



gi|75459781|gb|DT768009.1|DT768009 EST1201858 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZO67,

GCTCTTCCTCTTCCTCCTCTA
.||||||||||||||| || |
TGAGAAGGAGAAGGAGAAG-T

EST sequence (targeted fragment marked with yellow):
GTTTCGCGCTTCAATCAATTTTGATTTGAATTAAAGTTGTAGAGAAGAAGAAGAAGAAGAAAGATGGGAGAAAGAAAAGTATTGAATAAGTATTATCCACCGGATTTTGATCCAGCGAAACTTCCTCGAAGAAGACAACCTAAAAATCAACAAATTAAAGTGCGTATGATGCTTCCTATGAGTATTAGATGTAATACTTGTGGAAATTATATCTATAAAGGAACTAAGTTCAATTCGCGGAAAGAAGATGTTATCGGCGAGACATACTTGGGAATTCAAATATTTAGGTTTTATTTTAAATGCACAAAATGCTCAGCTGAACTGACGATGAAAACGGACCCACAAAATTCAGACTATGTGGTAGAGTCTGGGGCGTCCCGCAATTATGAGCCTTGGCGTGCCGAGGATGAGGAAGCAGATAAAGAAAAAAGGAAACGGGACAGTGAAGAAATGGGGGACGCGATGAAATCTCTGGAGAATAGAACACTAGATTCAAAAAGAGAAATGGACATACTTGCTGCTCTAGATGAAATGAAATCCATGAAGTCTAGACATGCTGGTGTCAGTTCAGAGGAGTTGCTTGAGGCCTTGCAACGTACAGCTGAAGAGAAGGAGGAAAAGTTGGAGGCAGAGGATGAAGCTCTGATAAAAGCAATTTATCAGGGTCCAAGAGCTATTATCCGAAGGATTGAAGATGATGATATTGATGAAGATGAAGATGAAGATGAAGAGGAAGAGGAAGAGTTACATATTCCAACCGACAATGGTGAAACAATTAATCACCAGGCCAAAAGGAGAAAAGTTTCAACTGACCTAGGGAATCCAACAGATTTTTTGACTCA



gi|71714775|gb|DR945412.1|DR945412 EST1136951 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1QS75,

GCTCTTCCTCTTCCTCCTCTA
||||||||||||||||| |
AGAGAAGGAGAAGGAGGAAAA

EST sequence (targeted fragment marked with yellow):
GAAAATAAAAAATGCAGGGCATTGCATACATAAATCAGACCTATACAGCTTTCTGCAGTTAAGTGATCATGGATTACATGAAGCCATTTTTGTTCATCTAAACCAGGAAAAAAAAAGCCGAGGTCAAGATGAAGAAGAGAGGGAAAAGGAGGAAGAGGAAGAGAAAGAAGAAAAAGAAGAGCGATGTAGGTGTAGTAGATAGAATAAGTGCTCTACCTGATGAAATTCTGCATAACATTTTTTCATTCATAGACATGACAACAATAGTTACAACAAGCATATTGTCGAAAAGGTGGAGGCATTTATGGACTTCTACTCCCTATTTAAGCTTTAATAGCCGTCAATTGTATTTTCAGTCAAAGAATGCTGATGGTTATGATTACATGCGTTTCAAACAATTTGTTGATAATGTGTTTTTACGTCGTGAGAATTCTTCTATTATATACAAGTTCGAAGTATACCTTGTTGGTCCTTTGTTCAGTTCATGTTTTCTAAACACATGGCTGGTGAAGTCTTTAGAAAGTCATGTTCAGATTTTTGATCTTGAGGTACATCAATATGATCATGATGAAGCTGCACGACTTGAATTGCCTGACTGCCTCTTTACTTCTGAAGTTAAAACATTGACGTTGGATGGTTTGTTCGTAATCCCAAATACGATGGGTTCTGCAGCCAACATTACAAGCTTGCATTTGCTGGCTGTGATACTTCCGGATGGGAATAGGAATGGGGAGCTCATTCTGAGCTTACCGGTTTTGGATAATTTAAAGATGCATTGGTGTCGTTATAATCATCTCAATTGTCTTACAATAAAATG



gi|75456678|gb|DT766195.1|DT766195 EST1200044 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZD51,

GCTCTTCCTCTTCCTCCTCTA
||||| |||||||| |||.|
AGAGAAAGAGAAGGAAGAGGT

EST sequence (targeted fragment marked with yellow):
AGCAGGAAGTGAAGGGAGAGGAGCAGCTAGGTATGTTAAGGAAGAAGAATAACTGCAAGAACAGCAGTAGTAGAGATGTTTTTTCATCTCTTTTGTCTTCTAATGATCGTGACTATCTGCTCTCTCCATCAGGAAACCAGGTGAAGGTATCAGATTTACAAGGAAAGGTTGTAGGACTTTACTTTGCAGCTAATTGGTACCTACCCTGTAGGAACTTTACTCCCATCCTAGTTGGTGTGTACGAACAATTAAGGAGTCAGAAAATTGGATTTGAGATCATATTTGTTTCGTCTGATGAGGACTTTGATGCCTTCAATCACTACCGAGCGACCATGCCTTGGTTGGCTATTCCTTTTTCTGATTTAGACTCAAGGAAAGCCTTGAACCGAAGGTTCGATATCGAAGATATTCCATGTTTGGTCATTCTCCAACCAGATGGCAATGTAGTTAATAAGACATTGCACGACGGGGTTGAGCTCATATATCGGTATGGAGTTCAAGCCTTCCCATTTACTAATAAGAGATTGAAAGAATTGGAGAAGGAAGAGAAAGAGAAGCATGAGAATCAAACCATAACCAACTTACTAACAAGCCCTGATAGAGACTACCTATTGGCTCAGTCCACAACTAAACAGGTTACAGTGGCATCATTGGTAGGAAAAACTGTGGGGCTATACTTCTCAGCTCAATGGTGCCTTCCATGCCACAAGTTTACTCCCAAGCTCATATCCATCTACCATAAGATAAGGCATATGTTCATCGATGATATGGACAAGACTTTGAAATAGTATTTGTGTCAAGCGATCGCAACCAATCCGCTTTTGC



gi|74561448|gb|DT759503.1|DT759503 EST1193352 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1Y922,

GCTCTTCCTCTTCCTCCTCTA
|||||||||||||||||||||
CGAGAAGGAGAAGGAGGAGAT

EST sequence (targeted fragment marked with yellow):
TTTGTATTAGGAACAACAACAACAAAACTCTAATAACTAACCCTTCTTTCTTGAATTAGAGTTTCTTTAAATCTCAAGATTCGAATTGATTTTCTTTTATGTTTATAGATTTCCTTTCTTTTCTTGAAATTTTCTTGTTTAGATCTGTATAGTTGTTTTATTTCTAAAGTTTTTGTAAATTTTTCAGGCTTTTGATCATTATATATATATTTTTTTAGATCATTGCCAAATAACATATAATCTATGGATGTTCTTTTGATATCTCCAACACCAGATCAATCTATTTCTCCTTATAAAAAGAATAGAAAACATCGTGGAATTATTAACAACAACAACAACAATCATGCTTTTATTTCATCTCCTAATGAAAATTTCTCTGGTTTTGATCAAAACATTATTAATGGAGGAGGTGGAAGAAGAGGTGGTTTTTTAATCTTACCACCACCACCATCATTTCAATCTTCTTCTCCATGTCGACAACCACCTCTTCTTCCTCTTCCCATTTCAAAACCTTATTCTTCTCTCAACAATCTTTATAGCGGAAACAATAGAGGAGGAAGAGGAAGAGCATTATCAACAAGTCCTCCAACTAACAAAAAAAATCTCATTCCCAAAAAACCCACATCTTCAATTAGTCCAAAGAAACAAGAATCTACAATGAAATCTTTGAAATCCAAGACAGTTAATAGATTGGGACCAGAACCAGGTAATCTTCCCAAGGAAGTTTCCAAGGTCATGAGGTCTGTTTCTACTCTTACTGCTGCTATGGAAGATGAAGAAGAAAATGAACGTGGTAGTGTTTTGGAAGAGATATGTGGTTCTTCTATCTTTTTATTGGCTCCA



gi|74562544|gb|DT760127.1|DT760127 EST1193976 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1YC81,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||| |||
GGAGGAGGAGAAGGAGAAGAA

EST sequence (targeted fragment marked with yellow):
CCGAGTCCAGTATAATTGAGAAAGCTGGAGCTGCTTTGGAGGTTTTGAGAGATGTGCTGGATGCAATTGATAGCCAGAATCCTGAGGGAGCAAAAGATGAATTCACGCTGGATCTTGTGGAACAATGTTCATTTCAAAAGCAACGTGTAATGCATCTTGTGTTAACATCTCGGAATGAGAATGTGGTTTCTCAAGCAATTAAATTGAACGAGCAGCTGCAAAAAGCGTTAGCTAGACATGATGCTCTTCTTTCTGTTAGGGCTACTTCTACTGCCACTCAATTTGTCCATGAAGAAGAGGAAGAGGAGGAGGATGAGCTGCTTTTTCGAAGACTCAGAAAAGGAAAAGCTTGCATGAGACCTGAGGATGAAGACCAGTTGCAGAGACCTATGGGCTTACTTGGATCCACTGACGGTGAAAGGCTGCAACGTCCACTTATACGCCCTTTGTGTTTGGAGCCAAAGGAATCTGGCCCACCTGTTCCTGCTGTCATTCCTCCTCCACCTGCCAAACACGTCGAGAGAGAGAAATTCTTTAAGAAGCGGGTGGACGGTTCGACATTAGAAGGTAATATCAGTGGCCTCACATTGCATAGTCGTAATGCAAGCAGTTCGCACAGTGAAAGTCTGGATTCTAGTGAATGACTGGGCATTTTGGATGTGAAGTCTTGCTCTTTATGCATTGTGGGATGGATCTGGACTATTAACGAGCTTGTTGTACATGTCCGAAGAGTTCCACTTCCAAATACGCATAGTTTGTGTTTCATGGCGTTTTTTATGTGCAACTGCTGCCTTTTTATGTGCTACTGCTGCCTATATGGTTTAGCAGCATGTGTTTTGGT



gi|74520803|gb|DT734509.1|DT734509 EST1168359 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1TS18,

GCTCTTCCTCTTCCTCCTCTA
.||||||||||||||| ||.
TGAGAAGGAGAAGGAGAAGGA

EST sequence (targeted fragment marked with yellow):
AGATTTCTCTCTAATTAGGGTTTCGCGCTTCAATCAATTTTGATTTGAATTAAAGTTGTAGAGAAGAAGAAGAAGAAGAAAGATGGGAGAAAGAAAAGTATTGAATAAGTATTATCCACCGGATTTTGATCCAGCGAAACTTCCTCGAAGAAGACAACCTAAAAATCAACAAATTAAAGTGCGTATGATGCTTCCTATGAGTATTAGATGTAATACTTGTGGAAATTATATCTATAAAGGAACTAAGTTCAATTCGCGGAAAGAAGATGTTATCGGCGAGACATACTTGGGAATTCAAATATTTAGGTTTTATTTTAAATGCACAAAATGCTCAGCTGAACTGACGATGAAAACGGACCCACAAAATTCAGACTATGTGGTAGAGTCTGGGGCGTCCCGCAATTATGAGCCTTGGCGTGCCGAGGATGAGGAAGCAGATAAAGAAAAAAGGAAACGGGACAGTGAAGAAATGGGGGACGCGATGAAATCTCTGGAGAATAGAACACTAGATTCAAAAAGAGAAATGGACATACTTGCTGCTCTAGATGAAATGAAATCCATGAAGTCTAGACATGCTGGTGTCAGTTCAGAGGAGTTGCTTGAGGCCTTGCAACGTACAGCTGAAGAGAAGGAGGAAAAGTTGGAGGCAGAGGATGAAGCTCTGATAAAAGCAATTTATCAGGGGTCCAAGAGCTATTATCCGAAGGATTGAAGATGATGATATTGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGAGGAAGAGGAAGAGGAAGAGTTACATATTCCAACCGACAATGGTGAAACAATTAATCAC



gi|71703911|gb|DR934548.1|DR934548 EST1126087 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1OX66,

GCTCTTCCTCTTCCTCCTCTA
|||.|||||||| || ||||
GGAGGAGGAGAAGTAGAAGAT

EST sequence (targeted fragment marked with yellow):
CAACTCTCGCAAACATCGAGACTTCGTAGGACTGACGGAACCCTAACGGAAATTACTCAGAAACCGTTAGGTGGTGTTGGTGTTGGTGGTGTAGTATTTTCTAATAAAAATCCTCTTACTTCTCCACCTCTTACCTCTTCTTCTAATTCTTCTTCCGTTACCGTTTTCTCTTTCAATGATTCATCCACCGCTGACGTCATTCTTCGTCTATATCTCGACTCTTCTATGCCCGATCCTGACTCAGACGATCCCGATTCAATCTCATCTGCTGACTCAGCTAATCAAGCTGATGTTAAGCTTTATCTTCATTCAAATGTTCTTCGAAGATCTTTATACTTCTCTGCTCTTTTATCTGATCGATGGCAAAAAAAGCAATTAGAAGATGAAGAGGAGGAGGAAGAAGAAGAAGAAGATGATGATGATGCAGAGAAAATTGTTTTTAATCATGTTTTAAAAGTTCCTGCTTCGATTGGATCAATTGATTCTCATCTAACGGTTCTACAGCTTCTTTATACTGATGATTTCTCAGGAATCGTTGATTCTGCTTGTTCTGCTCTTTCTATACTTCCTGTTGCTCTTGAGCTTCTATTTGAGGATTGTGTTAAAGCTTGTGTTCGATTTCTTGAAGTTGTTCCATGGACTGAAGAGGAAGAAAATAGAGTTTTAAGTATAA



gi|75460030|gb|DT768170.1|DT768170 EST1202019 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZP60,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||||||.
GGAGGAGGAGAAGGAGGAGGA

EST sequence (targeted fragment marked with yellow):
TCGCGAAAAACGTTTTGAGTTTCTTCTCTCTCTCTCTCTCTCTCACCTTCGCTTCAACTCTTCGATTTCGAACAAAGCTCTAACTTTCCTCTCTGTTGCTTTGCAAGCCTACTTTCTTAGAAACAGAAGATGCCAAGAGGAAGGGGGAGTCGGAAGGACGTTGAACCAGAAAAGCCTACTGAGCCCGAGAATCCAGTTGAACCCGAAGAACAGGTGGACCTTGATGGGGACAACGATGCTGAGGAGCAGATGGAAGAAGAAGTTGAGTATGAAGAAGTAGAGGAAGAAGTAGAGGAGGAAGAGGAGGAAGAAGAGGAAGAGGAGGAGGAAGAGGAGGAGGAAGAGGAAGCTAATGGAGTTGATGAACAGGCAAGCCCTGATGACGATCAAGATATGAAGACTTCAAATGTTGATGACGAAGAGAGCAAAAAACATGCTGAGCTTCTTGCGTTACCATCTCATGGTTCTGAGGTGTATGTTGGTGGCATTCCACATGATGCTTCGGAGGAGGATTTGAAGGGTTTTTGTGAGTCAGTAGGAGAAGTCACTGAGGTTAGGATGATGAAAGGCAAAGATTCGCGGGAGAACAAGGGTTATGCATTTGTAACATATAGGACTAAGGATTTGGCTGCCAAGGCCATCAAAGATCTGAATGGTACTGAATTTCAGGGCAGAAAGATAAAATGTTCAACCTCTCAAGCGAAGCATAAGTTGTTCATTGGTAATATTCCTAGAAGTTGGACAGAGGACGATTTACAGAAAATAGTGGATGATGTTGGACCCGGAGTTAATGGCGTGGAGTTGCTGAAGGATCCTCAAAACTCCAACCGTAATCGTGGTTTTGCTTTCATCGAGTACTATAACC



gi|71704574|gb|DR935211.1|DR935211 EST1126750 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1P253,

GCTCTTCCTCTTCCTCCTCTA
|||.|||||||||||||||
GGAGGAGGAGAAGGAGGAGAA

EST sequence (targeted fragment marked with yellow):
GAGTGAGAGAGAAAGAAAGAAAAAGAGAGAGAGAGAAATGGAGGTTCTGAGAAGAGGAGGAAGAGGAGGAGGAATGGGGTTGCTGACTTTACTCTTGGTGCTAACATGCGCATTGGTGTTTATTGTACCAGAAATTTCAGCTACTCGTTACATTGTTGGTGGAAAGTTTGGTTGGAGTCCTAACAATGTTAACTTTACTGATTGGGCTAAAGAACATAAGTTCTACCTTGACGATTGGCTCTGTAAGCTCTTTTCTTTCTTCTCCAACTTTCTTTTCTGCCACTTTTTTCTTCAAATAAGCTTTTTTTTCAAGATTCTTAAGCTGATTACAAGCAGATCTAGATATGTTTTTAGTGTTCTTTCCTTTTCTTGAGCTAGAAAGCTTTGCTTTACCTTTTTTGCTTGAACTCACTCATTTTAGTGAGAATAGCTAAAGATCTTTGCTTTTTTTCATGTTTTCCCCTCTTTTTTGGTTCTGGGTTTAGCTCAATTTTTTGTGGAAATGTGGGTTTTCAAGATTATGATTAATTGAAGTGCTAAATAAGTTAAGTGCATTATTTCGATCTATTTCATGACAAGTTGAATTATTACTAAAAATGAAAGATTGAAAGCTTTTTCCTCTGTTTTTTTATGTGAAAAATAATTCAGTTTTCAGATCTTCAAGCACAATTGACACTTGATGCACCAATTTGGCTTACTTGAAGTGGAGAATCAATGTTCTTTATGTAGTGTGTATGAGTTCAACAATCAACAACCTTTTATAATTCAGACTAGTTACTCATCTGTTTTCGTATAGGAAACAACCTGTTCTGTTTTTCAAAATTCATTGTTTTAGCAAAACATTGCTGATTAGTAGTTCCCTATTTGATGAGAAGATTGTTAGAAAGTAGAAACCTATTTTCTTTGAAAATA



gi|74521099|gb|DT734678.1|DT734678 EST1168528 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1TT19,

GCTCTTCCTCTTCCTCCTCTA
|||.||||||||||| |||
GGAGGAGGAGAAGGAGAAGAA

EST sequence (targeted fragment marked with yellow):
GATAAACCTTCCCAACCCCATCTTCTTCCTCGTGTTTCTTCTTGTCCACGTCATCCTGACCATCAATTCACTGGATTCTGTCCTTCATGTTTACGAGAACGTCTTGCTGGTTTAGATCCAGCTACAAATTCCACCATTGCTTCTTCTTCTTCTCTACTTAAGAACTCCTCTACTACTACTTCTTCTTTAAAATCTATTTTTAGAAGTAATGGCGGCGATAAAAATAAGGGTATTGCTTCTTCTTCATCTTCATCTTCAATGTATTTCCCGGAGTTACGTCGTTGTAAATCATTTTCTGGTGGAAGAGGTGAGAGTAATTTTATTTCTAGTAATGTATTAGAACCTAAAAGGAAATCTTGTGATGTTAGGGTTCGTAATACACTTTGGTCTCTTTTTAATTTGGATGATGAAAAGAAAGGAACTAATTTTAATGAGGTTTGGGGTGAAATTGAAATTGAAACTAGAGATTTAGGGTTTGGTGAACCTGTGATTGAATTGAAAGAAGAAGAAGAAGAAGAAGAGGAAGAGGAGGAGGAAGAAAGGGGAAATGGGGGTGAAATTAGGGTTTCTGATAATAACATTGTTGAAGATAGAATTGGAGAAATTGAAGAGGAATTAGTAGTAGATGGGGAGGAAATTAAAACCATGAAGGATCATATTGATATGGAAGCGCAGATTAAGAAGCCGCCATCTGGGAGGGATTTGAGGGAGATTGCTGGGAGTTTTTGGTTGGCAGCTTCGATTTTTAGTAAGAAATTGCAGAAATGGAGAACAAAACAGAAACTGAAGAAGCATGCTGGCGGAGAAGGGAATCTTCTAGAATGCCGAAGAAGAAGAAAGGGATGGGAAGACAGT



gi|71686811|gb|DR917448.1|DR917448 EST1108987 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1LV50,

GCTCTTCCTCTTCCTCCTCTA
||||||||||||||||| |
AGAGAAGGAGAAGGAGGAAAA

EST sequence (targeted fragment marked with yellow):
GTTCATCTAAACCAGGAAAAAAAAAGCTGAGGTCAAGATGAAGAAGAGAGGGAAAAGGAGGAAGAGGAAGAGAAAGAAGAAAAAGAAGAGCGATGTAGGTGTAGTAGATAGAATAAGTGCTCTACCTGATGAAATTCTGCATAACATTTTTTCATTCATAGACATGACAACAATAGTTACAACAAGCATATTGTCGAAAAGGTGGAGGCATTTATGGACTTCTACTCCCTATTTAAGCTTTAATAGCCGTCAATTGTATTTTCGGTCAAAGAATGCTGATGGTTATGATTACATGCGTTTCAAACAATTTGTTGATAATGTGTTTTTACGTCGTGAGAATTCTTCTATTATATACAAGTTCGAAGTATACCTTTTTGGTCCTTTGTTCAGTTCTTGTTTTCTAAACACATGGCTGGTGAAGTCTTTAGAAAGTCATGTTCAGATTTTTGATCTTGAGGTACGTCAATATGATCATGATGAAGCTGCACCACTTGAATTGCCTGACCACCTCTTTACTTCTGACGTTAAAACATTGAAGTTGGATGGTTTGTTCGTGATCCCAAATACGATGGGTTCTGCAGCCAACATTACAAGCTTGCATTTGCTGGGTGTGATACTTCCGGATGGGAATAGGAATGGGGAGCTCATTTTGAGCTTACCGGTTTTGGATAATTTAAAGATGGATTGGTGTCATTATAATCATCTCA



gi|75460030|gb|DT768170.1|DT768170 EST1202019 Aquilegia cDNA library Aquilegia formosa x Aquilegia pubescens cDNA clone CO1ZP60,

GCTCTTCCTCTTCCTCCTCTA
|||||||||||| |||||.
GGAGAAGGAGAAGAAGGAGGA

EST sequence (targeted fragment marked with yellow):
TCGCGAAAAACGTTTTGAGTTTCTTCTCTCTCTCTCTCTCTCTCACCTTCGCTTCAACTCTTCGATTTCGAACAAAGCTCTAACTTTCCTCTCTGTTGCTTTGCAAGCCTACTTTCTTAGAAACAGAAGATGCCAAGAGGAAGGGGGAGTCGGAAGGACGTTGAACCAGAAAAGCCTACTGAGCCCGAGAATCCAGTTGAACCCGAAGAACAGGTGGACCTTGATGGGGACAACGATGCTGAGGAGCAGATGGAAGAAGAAGTTGAGTATGAAGAAGTAGAGGAAGAAGTAGAGGAGGAAGAGGAGGAAGAAGAGGAAGAGGAGGAGGAAGAGGAGGAGGAAGAGGAAGCTAATGGAGTTGATGAACAGGCAAGCCCTGATGACGATCAAGATATGAAGACTTCAAATGTTGATGACGAAGAGAGCAAAAAACATGCTGAGCTTCTTGCGTTACCATCTCATGGTTCTGAGGTGTATGTTGGTGGCATTCCACATGATGCTTCGGAGGAGGATTTGAAGGGTTTTTGTGAGTCAGTAGGAGAAGTCACTGAGGTTAGGATGATGAAAGGCAAAGATTCGCGGGAGAACAAGGGTTATGCATTTGTAACATATAGGACTAAGGATTTGGCTGCCAAGGCCATCAAAGATCTGAATGGTACTGAATTTCAGGGCAGAAAGATAAAATGTTCAACCTCTCAAGCGAAGCATAAGTTGTTCATTGGTAATATTCCTAGAAGTTGGACAGAGGACGATTTACAGAAAATAGTGGATGATGTTGGACCCGGAGTTAATGGCGTGGAGTTGCTGAAGGATCCTCAAAACTCCAACCGTAATCGTGGTTTTGCTTTCATCGAGTACTATAACC