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mature miRNA: GCTGGGACGGAGGGAGTACAT
species: Pseudoroegneria spicata
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|207425578|gb|FF363119.1|FF363119 BG02037B2F10.r2 BG02 - primary and normalized libraries Pseudoroegneria spicata cDNA clone B

GCTGGGACGGAGGGAGTACAT
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AAACCCTGCCTCCCTCATTTG

EST sequence (targeted fragment marked with yellow):
ACCGATTTTTNNNTTTTTTTTTTGCCCAACATCATTTTGTTTACTCCCTCCGTCCCAAATTATTTATCTTAGATTTGTGTAGATACGATTGTGTCTAGCTAAATCTAGTAATTTGTGACACAGGTAATACATGACTCAAGACACGATGGTCTAAGTGCAGCATCAGATCACAAGTTCATCAAAAAGAGCTCAGCGCAAATTGACCTAGTAGCTCAGACTCCAAAAAGAACCATCGGATCACAAGATCCTCGAAAAGAGCTCGAGCCTGGTAGCTCAGACTCCAGAACTCAAACTCCATACGACAAAGTTTTGGGAACTGAGAAAAATCTTAAACCAAAAGAAAAACTGGAGGGGACAAGCCGTAGGAAGCATCAGCTGTCAGGTATGCCCACCTACCTTGAAAAGAGGGCAGCTCTCAGTTTTAGCTTTAACCTTAGGAACCATAGAAGCTCCCAGGGGAAGAGTAACGTCGGACAGGACTCCAAGGGCTCCTCCCACATGAAACGTATTCCTCGGCATCACGAAAAGTTTACTGTAGCTGCGCCATGTCATGCCCCATTGGCTGTCGAATGAATTCATACCGATCCAGTATTCGCCCTGTAGTGGGCACTCACCATATCCAAGTAGAAGAACAGAGTGATCAGGTTTACCATGTTTGTCAGGAACTACAAGGAAATCCTGTTTGTAATTCATAAATTCGCCGTCAGTGTAGATTGAAGAAACCACAGGCTGATGAGCAACTGCATACTTCACAGCTTCTTCTGTCGGTTCTACAG