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mature miRNA: GGAATGAGCAGGGACATGGTG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52401833|gb|CV239301.1|CV239301 WS0231.B21_P20 PT-MB-A-13 Populus trichocarpa cDNA clone WS0231_P20 3', mRNA sequence

GGAATGAGCAGGGACATGGTG
|| |||||||||. ||||.|.
CCATACTCGTCCTGGTACTAT

EST sequence (targeted fragment marked with yellow):
AGAAAACATCGGGTTGCTTGGAAGCACTCGTGTCGGAAGCCGCAGGGTTATTCAAATCTCAGCTGGTTTTATGATATTCTTCTCTATTTTAGGAAAATTTGGAGCTTTATTTGCATCAATACCCTTCCCTATTTTTGGTGCTGTCTATTGCGTGTTGTTTGGTCTAGTTGCTTCCGTGGGGTTGTCATTTTTGCAATTCACAAACATGAACTCAATGAGAAACCTTTTCATTACTGGCGTTGCCTTCTTTCTTGGTTTGTCCGTCCCTGAGTACTTTAGAGAATACACTTCCAAGGCCTATCATGGTCCTGCTCATACCAGAGCTGGATGGTTCAACGATTTCCTCAACACTATCTTCTTCTCATCCCCAACTGTTGCATTGATAGTCGCTGTTTTCTTAGACAACACACTTGACTACAAGGACAGTGCTAGGGATAGAGGAATGCCATGGTGGGTCAAGTTTCGGACATTCAAAGGAGACAGCAGGAATGAAGAGTTCTACACCCTTCCTTTCAACCTTAATCGTTTCTTCCCTCCATCTTGAACTAACTTCAATTCCAACGAAAGATGATAGAGGAAAAACATCATTTCCAGATCAGGAATATATGTGGGGTATTACAACTATAGTTAAGAAGAGTGAAACAAAAGATCCATGTGTTTTGCTTGCAGAGAATGACTCCTTCCTGCTTTCTCCTCTCTCATTTTCATTGATTTTGTTATGTATATGACTTGGGTTGGTCAGGTTTATTACAACAAAACTAATTTATCACCAAAAAAAAAAAAAAAAAA