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mature miRNA: GGCTCGGTTCAGTTCGGTTT
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73868520|gb|DT471258.1|DT471258 WS01213.BR_C19 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS01213_C19 5', mRNA sequence

GGCTCGGTTCAGTTCGGTTT
.||||.|.||||.||.|.||
TCGAGTCGAGTCGAGTCGAA

EST sequence (targeted fragment marked with yellow):
GGCTCTTTTGCTGAAAACTGAAAAGGAAGGTTGGAAAAAAAAAAAAAAAAAGGCTGTGTAGATAGCCAAAAAAACAGTGTGGCGCTAGCAACTGCCTTTTACTTGCGTCCCTGACTCCAAGTATACAAATTAAAAATGCCTTCTTCTTTCTCAGCTCCAATCATCAGAAGCTGAGCTGAGCTGAGCTTCAACAACCCTCCCTCTGTCTTTATTTATTATCGATCTCCCTCTTATTATCATACTTCTTCTTCTTCTTCAATCATTATATATATATTACATTACAACTCTCAAAACAACTAGAAAAAAGAAAATGTCAAAATCAGACTCTTCCCCGGAAACAGCTCGCAGCNCGCTTGGCCGTGCTCACCGCTCATTTAGTTGGAGCAACGACACTCGAGTCATCTTCGTCGTCGATTAACCGGTCTTGGTGGTCTGCGCAGGTCTCTCCTCCTGGAAACCTCCGGGGCGCTTTGACGGTGATCGATGAGAGGACTGGTAAGAAAACCCAAATTCCGGTCTCTCAAGACGGCACCGTCAAAGCCTCTGATTTCAAGAAGATCTCGACAGGAAAGAATGACA