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mature miRNA: GGTCTGGTAAAGATGTCAGC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52399497|gb|CV238135.1|CV238135 WS0125.B21_H02 PT-GT-FL-A-3 Populus trichocarpa cDNA clone WS0125_H02 3', mRNA sequence 

GGTCTGGTAAAGATGTCAGC
..||||.||||.|..|||.|
TTAGACTATTTTTGTAGTTG

EST sequence (targeted fragment marked with yellow):
AGGAAGCTGCTGAGAGCACTTTGTTGGCTTACAAGTCTGCTCAGGATATTGCACTTACTGAAGAGCTGGCTCCTACTCACCCAATAAGGCTTGGGCTTGCTCTTAACTTCTCTGTCTTCTACTATGAGATCCTTAACTCTCCTGATCGTGCTTGCAATCTTGCCAAGCAGGCTTTTGACGAGGCTATTTCTGAGCTGGATACATTGGGTGAGGAGTCTTACAAGGACAGCACATTGATCATGCAACTTCTCCGAGATAATCTGACACTCTGGACCTCTGATATCACGGATGATGCGGACGAGATCAAGGAAGCATCAAAACGTGAATCCGGCGAGGGGCCGCAGTGATGAAGTTCAGTTCATAGGATATATGCACTCTGTACTTCTATCTTGTGAAATTTACAGACGCCTGTGGGTTGTTGTAGTTGGATTTATTAATGAATTTGATAATCTTGAGTGCCGTGCCACAGTACTTGGGATTGAAATGTACCAACAGATTATCAAGTTTTTAGTATTGATGCCTACAATCTGGGTTGATGTTTTTATCAGATTTTCATCTTCCAAAAAAAAAAAAAAAA


gi|73866663|gb|DT469401.1|DT469401 WS01917.C21_K16 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS01917_K16 3', mRNA sequence 

GGTCTGGTAAAGATGTCAGC
..||||.||||.|..|||.|
TTAGACTATTTTTGTAGTTG

EST sequence (targeted fragment marked with yellow):
TTGCAATCTTGCCAAGCTGGCTTTTGACGAGGCTATTTCTGAGCTGGATACATTGGGTGAGGAGTCTTACAAGGACAGCACATTGATCATGCAACTTCTCCGAGATAATCTGACACTCTGGACCTCTGATATCACGGATGACGCCGATGAGATCAAGGAAGCATCAAAACGTGAATCTGGCGACGGGCTGCAGTGATGAAGTTCAGTTCATAGGATATATGCACCCTGTACTTCTATCTTGTGAAATTTACAGACGCCTGTGGGTTGTTGTAGTTGGATTTATTAATGAATTTGATAATCTTGAGTGCCGTGCCACAGTACGTGGGATTGAAATGTACCAACAGATTATCAAGTTTTTAGTATTGATGCCTACAATCTGGGTTGATGTTTTTATCAGATTTCCAAAAAAAAAAAAAAAAAA


gi|73866489|gb|DT469227.1|DT469227 WS01917.C21_C11 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS01917_C11 3', mRNA sequence 

GGTCTGGTAAAGATGTCAGC
..||||.||||.|..|||.|
TTAGACTATTTTTGTAGTTG

EST sequence (targeted fragment marked with yellow):
GCATGCAACTTCTCCGAGATAATCTGACACTCTGGACCTCTGATATCACGGATGACGCCGATGAGATCAAGGAAGCATCAAAACGTGAATCTGGCGACGGGCTGCAGTGATGAAGTTCAGTTCATAGGATATATGCACCCTGTACTTCTATCTTGTGAAATTTACAGACGCCTGTGGGTTGTTGTAGTTGGATTTATTAATGAATTTGATAATCTTGAGTGCCGTGCCACAGTACGTGGGATTGAAATGTACCAACAGATTATCAAGTTTTTAGTATTGATGCCTACAATCTGGGTTGATGTTTTTATCAGATTTTCATCTTCCAATTCTGGGTTTTGATGTTCCTTGTGGCTCTGCTATATGATATTACATCAGTGACGAAACTACTTGTTTCGAATTATTAGCCAAAAAAAAAAAAAAAAAA