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mature miRNA: GGTTTTGAACCAGAAACCGG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|38574922|gb|CK090597.1|CK090597 F014P94.3pR Populus flower cDNA library Populus trichocarpa cDNA clone F014P94 3', mRNA seque

GGTTTTGAACCAGAAACCGG
||||| ||||| |||| |||
CCAAACCTTGGACTTTAGCC

EST sequence (targeted fragment marked with yellow):
CCCTTCCAAAGGCTTGTGAAAAAAATTGCTCAGGATTTCAAGCCCGATTTCAGGTTCCAAACCAGTGCTGTCCCAGCTTTTCAAAAACCACCCGAGGCTTATTTCGTGGGTCTTTTTGAGGACCCAAATTTGTGTGCTATTCATCCCAAAAGGGTTCCAATCATGCCCAAGGATACCCAGTTGGCTAAAAGGATCAGGGGCGAAAGGGCTTAAGTTGCGGGGCTTGTTAAAATTGTCTGGGTCTCATGTTTTTAGGGTTTATCGTTTTTTTTGTTGCTAATTTTTAGGGAAGCAAAGATCTGGCTAGATCTACTATTAGAATCATTCCCTTATCTAGTGATCTTGATTTCAGTACTGAAAAAGGTAATCGTTTTAAATGCAAATGGAAAACGGACTTAAATGTTTTCCCT



gi|24070245|gb|BU878721.1|BU878721 V050F07 Populus flower cDNA library Populus trichocarpa cDNA 5 prime, mRNA sequence

GGTTTTGAACCAGAAACCGG
|..|||||||||||||| .
CTGAAACTTGGTCTTTGTTG

EST sequence (targeted fragment marked with yellow):
CCTCATAAGAGTTCTCTGCATACAGACTTTCAATATGAAACAATTGAAAGGAAATACGAAGCTGTGGATTGTGGTGGCAGCATATCTTTTGTCGACTGTACTCGTGGCGAGTACAGAAGCTGGGAACATGGACTCATTGGCAGAAGATCAAACTGGGAGGGACATGATCATCCGCTCTGTCGTGCCAAAAAATGACGATGTTGTTTCTGGTTCAAAGTCCACACAACACATTGTTGGCATAAATAATTTGTGCGTTGACGTGTTTCTTGAACTCTACTTTGATGAAAACATAGTACAGTTGTACCCGTGTAAATCTGACGGAGATGTGAATCAACAATGGAGCTTGGAGAAGAACGGAACCATTCAATCTAAAGGCAAGTGTTTGACCACAAACGGCACAAGTCCAGGAAGCTATGTATTTATCTCTGACTGCAACAAGGTGAAGGCTAGCGCCACAATCTGGAAAGTACAAAAGGATGGCTCCATCCTAAACCCCTCCTCGTCACTAGTTTTGACCTCAAAGTCCGGGAAAAGTGGCTCCCTACTCACCTTGGAAACCAATGTTTA



gi|52497876|gb|CV244901.1|CV244901 WS0255.B21_O23 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS0255_O23 3', mRNA sequence

GGTTTTGAACCAGAAACCGG
..|||.||||| .|||||||
TTAAAGCTTGGGTTTTGGCC

EST sequence (targeted fragment marked with yellow):
GCGAAAGTAGTAGCAGAGAAGAACAAATGTTCTTCACCATCTCAATCCTTCTCCCGTCACTTTTATCCAAGCAAAACCATGGAAACTTAAATACTTTGGCTGGAAGAGTGAAACCATGATCTAAACATCAAGCTAGAATAAATAGTGGAGAGAGAGGGTGTAAAGGTAGAGAGAAAGTGAAGAAATGGAAGGAAAATGGAGATTTGAGGTGGAGATCCGGTTTTGGGTTCGAAATTTGGCATCAAAATGACAAGGAAGGTGTGCTTAATCCACCTAGCTTACTTTGATTCCAATTACGAGAAACATTCAGAAAGAAACCCGACTTTTCAGAAGCTGGGATGACGAGAGTTAAGGTAAGGAATTGACCCACCCTTTCCTTTTTAATTATTTATACATGATTTTATAATGTTAGAATTATAATGGTAATTAAATTATATAGTGTATTGTTGTTGTTATTGTTAATGGAAGGCATATGCCTTCGAAGTAGAAATTGGTATGTAATTTGTAGTTAATCGCATAAGAGATAATGGGTAGTCTATTATGAGATGTGATTTACTGTAACGTAAAAAAAAAAAAAAAAAA



gi|3857628|gb|AI166343.1|AI166343 xylem.est.185 Poplar xylem Lambda ZAPII library Populus trichocarpa cDNA 5', mRNA sequence

GGTTTTGAACCAGAAACCGG
||||| ||||| ||| |||.
CCAAACCTTGGGCTTGGGCT

EST sequence (targeted fragment marked with yellow):
AAATACTCTTATAGTAGTATATACATACATAGTATCTGGTATTCTCGGACCTTCGGTTCCTAGGCTTAACAAATGGCCAATTTGAAGTGCTTGGCAGTTCTTGGTCTCCTCGTTGCATCTTTTGCTGTGAGTGAAAGCCGGGTCGCTAAACAAGACTTGGGCTTGGACCTTGGAGGCTTAGGGATTGGTTTGGGTGTTGGATTGGGTATTGGGCTGGGAGGTGGTGGATCAGGTTCTGGTGCTGGAACAGGTTCGGGTTCGGGTTCCAAACCTGGGTCTANCTCAATTTCCATTTCATCCTCTTCTTCGTCAANCTCTANTTCTGGGTCCGGANGCGGTTCTGGTGCCGGGTCAAAACCGGTTCGTCTGCTGGTTCTAGGGCCGGGTCAGGAACTGGTTCCTCT



gi|73887788|gb|DT490526.1|DT490526 WS02545.B21_D07 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02545_D07 3', mRNA sequence

GGTTTTGAACCAGAAACCGG
|| ||||||||| ||| ||.
CCTAAACTTGGTATTTCGCT

EST sequence (targeted fragment marked with yellow):
TTTATCATGGTTAAACCTGATGGAGTTCAACGTGGACTCGTTGGAGAAATCCTTAAGCGTTTCGAAGCTAAAGGTTTCAAATTGGTCGCATTGAAAATGGTTTGGCCATCACAACAATTGTTGGAAAATCATTATGCTGATTTGTCGGCTCGTCCATTCTTCCCAGGTTTGGTGAAATACATGAGCTCTGGTCCAGTTGTTGCTATGGTTTGGGAAGGTTTGAATGCCGTTAAAACTGGCCGTCAAATGTTGGGCGCTACCAATCCAGCAGACTCATTGCCTGGTACAATTCGCGGAGACCTTTGCATCCAAGTTGGCCGTAACATTATCCATGGTTCAGATGCCGTTGAATCAGCCAACAAAGAAATCGCTTTATGGTTCAAATCCGAAGAATTGGTCTCATGGAAACCAGCCAACCCCGACTGGATCTACGAATAAATTGATGCCGGATTCCATTTTATTCAATTTTTTTTTTCTTCAATGAAACATCCAATTATTTGTGATTCTTGAGCATCTTTAACATTGCAACGGAATTTCGAATAAACAAAATGTTATCATTTTAATTCCATCAAAAAAAGCATTGAGCGAATTGCATTTATTGAAGATTCTTTCTGAGAATTGTTTTTTTGTTTTTTTTTTTTTTGTTTTTAAATAATTCTTTTTCTTTCTTCGAGCTTAGAAAAAAAAAAATACTCTAAATTTAATAAAGTACGAACTGAATCAACTCAAACATAAAAAAAAAAAAAAAAAA