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mature miRNA: GTATCAGGACATCCAGCCCAA
species: Solanum tuberosum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|66840060|gb|DR038165.1|DR038165 51483.2 Late Blight-Challenged Tubers Solanum tuberosum cDNA clone 51483 5', mRNA sequence

GTATCAGGACATCCAGCCCAA
|||||||||||||| ||||||
CATAGTCCTGTAGGGCGGGTT

EST sequence (targeted fragment marked with yellow):
GTTCTTTCCAAGTGGTTCTCCTATTCGTCTTAATGCTTTTAGTGATTCTGATTGGGCGGGATGTCCTGATACTCGTCGTTCAGTCTCTGGTTGATGCATGTTTCTTGGAGAGTCATTAATATCTTGGAAGAGTAAAAAGCAAGATCGTGTGTCAAAATCTTCAACAGAGGCTGAATATCGATCCATGTCTACTGCTTGCTCCGAGGTTGTATGGCTTCGCAGATTACTTGCTGAGATTGGATTTCCCCAATCTCATCCTACTCCTCTCCACGCTGACAATACAAGTGCTATTCAGATTGCTACTAATCCAGTTTTTCATGAGAGGACCAAACACATCGAAGTGGATTGTCATTATATACGAGAAGCTGTAGATAAAGGAGTCATTACTCTTCCACATGTGTCCAGTGATCTTCAAATAGCTGATGCATTTACAAAATCCATGTCACGACAACGCCATCAGTTTCTAGTAGGCAAATTGATGCTTCTTGATCCACCAGCATCAATTTGAGGGGGGATGTTAGCATGATAGATAACTAAATGATTGTATATATTAATGTAATTATGTAGTGATATAAATTAGCATGATCATATACATTTCTTGTAGAATCATATGAATAAATATTGTGATAGAATAGCTGATTTGGAGGGATAGCAAAGGCAGATTTAGAGGGATTCAATGGCAGATTTGTATAGATAGAATAGTCATCTTTAGGATAGGAATGGCGGATCTTTAGGATGTGTAATCTTTATTTCTCTA