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mature miRNA: GTGAAGTGTTTGGAGGAACTC
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|31346916|gb|CD431273.1|CD431273 ETH1_7_D01.g1_A002 Ethylene-treated seedlings Sorghum bicolor cDNA clone ETH1_7_D01_A002 5',

GTGAAGTGTTTGGAGGAACTC
.|||||||||||| |.|||||
TACTTCACAAACCCCTTTGAG

EST sequence (targeted fragment marked with yellow):
AAGTGAGCATTTCCTCTCCCTCTCCGGGGCGGGGCTCATCCATGCACGGGGGCGGGATGGGTGTCTTCTTCCTCTAACCGGCCGGCCTCTTGCCAAGAGAATCATATCGCCGTCCATGCATATATGTGATATCCGTCTCCCAGGGCCGGTGATGTTTACACGAGAGGAGGAATTCTAGCCTGCCTCTTCCTCTGATCGTTGTCAGCAAGATTCTCCGTCCAACCGCCAGAACCAAAATCCCTTTTAAAGCAGGGGAGCTCCCAGCTCCCGAGTTTCCCCAAACACTTCATTTCAGCCTTCAGCTATGGAGGCATCATCGGCATCAGCGGTCGAGTCGACCGCCACCGTGTCCGTCTTCGATGTATCATCCAACAAGCAGCGCCCAGACACGGCGAAGCTCGTGCTGAACAGCCCCAGCCCGCCGAGCCTGCGCGAGGAGCTCGTCGGCGTGGTCGGCAAGGCGTTCCGGCCACGGCCCCCCGGCGGCGGTGGCCGGGCGCCGCGCTGCCCGTGGATCCTCACGGCGCTGCAGTGCGTGTTCCCGGTCCTGCAGTGGAGGAGGACCTACACCCTCAAGTCCTTCAGGAGTGACGTCATGGCCGGCCTCACTCTTGCCAGCCTTGGCATCCCTC



gi|9850614|gb|BE595541.1|BE595541 PI1_56_H08.b1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence

GTGAAGTGTTTGGAGGAACTC
.|||||||||||| |.|||||
TACTTCACAAACCCCTTTGAG

EST sequence (targeted fragment marked with yellow):
GCACGAGGTGAGCATTTCCTCTCCCTCTCCGGGGCGGGGCTCATCCATGCACGGGGGCGGGATGGGTGTCTTCTTCCTCTAACCGGCCGGCCTCTTGCCAAGAGAATCATATCGCCGTCCATGCATATATGTGATATCCGTCTCCCAGGGCCGGTGATGTTTACACGAGAGGAGGAATTCTAGCCTGCCTCTTCCTCTGATCGTTGTCAGCAAGATTCTCCGTCCAACCGCCAGAACCAAAATCCCTTTTAAAGCAAGGGAGCTCCCAGCTCCCGAGTTTCCCCAAACACTTCATTTCAGCCTTCAGCTATGGAGGCATCATCGGCATCAGCGGTCGAGTCGACCGCCACCGTGTCCGTCTTCGATGTATCATCCAACAAGCAGCGCCCAGACACGGCGAAGCTCGTGCTGAACAGCCCCAGCCCGCCGAGCCTGCGCGAGGAGCTCGTCGGCGTGGTCGGCAAGGCGTTCCGGCCACGGCCCCCCGGCGGCGGTGGCCGGGCGCCGCGCTGCCCGTGGATCCTCACGGCGCTGCAGTGCGTGTTCCCGGTCCTGCAGTGGGGGAGGACCTACA



gi|14366926|gb|BG948737.1|BG948737 LG1_330_D08.b1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA sequence

GTGAAGTGTTTGGAGGAACTC
.|||||||.||||||||||||
TACTTCACGAACCTCCTTGAG

EST sequence (targeted fragment marked with yellow):
CGCCACCGGCCGAGCCGAATCGAGCAGCAGCAGCAGCAGCAGCGCCCTCCCACCTTGCCATGGCGACGCAGGCTGCTTTCCTCGCGGGGTTATCGCAGCTCGCCGCACAGCCGGGCCGGGACCGCGCCGTGGCGCCGGCGCGGGTGGCCGTGGCGGCGGTGGGTGTGGGTAGCGCCAAGGTGGGTGTGCGGGTGGGCGTGAAGGCGGGGACCGCCGAGGCCAAAGCGGTGGCGCAGCGCCGCGGGGTGCGGTGCCGGGCCAGCCTGATCGAGCCCGACGGCGGGCGGCTGGTGGACCTGGTGGCGCCCGAGGAGGGCGGGCGGCGCGCGGCGCTGCGGCGGGAGGCGGCCGAGCTGCCGCACCGCCTGCGCCTCAGCCGCGTCGACAAGGAATGGGTCCACGTCCTCAGCGAAGGGTGGGCGAGCCCGCTGCAAGGGTTCATGCGCGAGCATGAGTTCCTCCAAGCACTTCATTTCAATGCCATCCGCGGCGCGGATGGCAGGATGGTCAACATGTCCGTCCCCATCGTGCTCTCCCTTGGGGACGC



gi|57810088|gb|CX611368.1|CX611368 ANR1_24_F08.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone ANR1_24_F08_A002 3', mRNA sequ

GTGAAGTGTTTGGAGGAACTC
|||||..||||||.||| ||
AACTTCGTAAACCTTCTTCAG

EST sequence (targeted fragment marked with yellow):
GCATCTGAAGTGCTAATCCAATGCTGAAGTGGTGAAAGAAAGAATTTAGAACATCTGGACAACACATGTTTGGGTTCCAGTGTCCAATTAGTCAGAAAATGGAGTCGCTCTACACCTAGCAGTCTAGGGCAGTGGCTATTGTACATAGTTACATATTGGGAGCAATGCAACCCTGTCCGGGGACACAAAAAAAAAGAAGAAATACATTCATGAGCAATCAGAGAAACAAAACATGAAACGCCTCCAGCCATCCAATATTTACAATCACTTGCGGAGTCCAGGATCAAGTGTCTTGATACTCTGGTCTATATGCCGGATAATGCAAACAGAAACAGCAGCAAATAGCTTGCGGCTCTCGCACCACAACCGCAGACTTCTTCCAAATGCTTCAACAATTGGTGGGCAGGCAGGCAGACGGGTTGGAAACAATCAGATCACTGCAAAAACATTATCTGGAAAGGAGTGATTGTCCCTGGCGCTTGGTCAGTAACAACATGTAAGCCTTCCTCAACAGCACGTTTACAGAATTGCAGGAGGTCCGGCCATCTGGTCCTCTCTTTTTTTTCCCTGGGTATGGTAGACTAGCTTCATGGCTCTTGCTTCGTATGATGAGAAATGCTTGCCGTACCATATGATGCAACCAGTCTGTTCAGTTGAATAAGTGAATTTATATGGTCTTCATTTATGGATAAAGGCAATGGAGGCATCTGACCCATGATACCAGGCGTTTCACTCATTCTGCTTAAAATTGTTTTG