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mature miRNA: GTGGGCCACACTTGTTGTCAA
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73881449|gb|DT484187.1|DT484187 WS02524.B21_L03 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02524_L03 3', mRNA sequence 

GTGGGCCACACTTGTTGTCAA
 ||.||||||||||||.||||
AACTCGGTGTGAACAATAGTT

EST sequence (targeted fragment marked with yellow):
GGATTTCATAGTAATCACCATTGATGACCCACCAGAGAACTGTCTACATTTCAATGTGCTTGTTGAGGCTAACGAGAGTGAAATGCCAACAGTTTCAATCCATCCGGATGATCCTGTGGCATTACCATTCTCTTCAGGGACAACAGGGCTCCCAAAAGGAGTGATACTGACTCACAAGAGCTTGATAACAAGTGTGGCTCAACAAGTTGATGGAGAGATCCCAAATTTATACTTGAAACAAGATGATGTCGTTTTATGCGTTTTACCTTTGTTTCACATCTTTTCATTGAACAGCGTGTTGTTATGCTCGTTGAGAGCCGGTTCTGCTGTACTGTTAATGCAAAAGTTTGAGATCGGATCACTGCTAGAGCTCATTCAGAAACACAATGTTTCGGTTGCGGCTGTGGTGCCACCACTGGTGCTGGCGTTGGCCAAGAACCCAATGGTGGCGAACTTCGACTTGAGTTCGATCAGGGTAGTCCTCTCAAGGGCTGCGCCACTGGGGAAGGAG