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mature miRNA: GTTCGGTTCGGTTCGGGTCAT
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124687335|gb|EH782075.1|EH782075 CNSS20913.b1_B21.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS20913 

GTTCGGTTCGGTTCGGGTCAT
.||||||| ||||||||| ||
TAAGCCAATCCAAGCCCATTA

EST sequence (targeted fragment marked with yellow):
GAAGGCATTTGTATTTGTGGTTGTATTCAAGCCAAAGCAGTGTGATATCCTTATTTTTAAAGGATTTTAATCTTATGATTTAACTCATGCCAAAAGTGATCTTTGGCTTGAAGTGAATGAGGACAATATTACTGTATTTTCTCTTATATTTTCAGATATTCATAACATCAAACTGTCTGGAAGTGTACTTAAACTTGTTTTTACTACATCATGGTATTAATTACCCGAACCTAACCGAATTACGTAGGCAAAGAATTGTTGCTATACCATTGCTTTGGTTTCCAACTTTTTTAGAACTCTGGAGCAGCTGATTTGACCAGGTGCAGTTGCTAGTGCAAAATGCGAGTCTTTCAGATTCCCTACCATACCATCTGGAGTAGTTAAGGGAATTAGAAATTGTTTATAATCTTTTTTAAAATATTGGACATAGATTGTGATAGCAAGAAGTAGATGGCTAAAACTCTCCTTCAAATTACACATTTGAAGTGATTGAGATTGTTGACGAGGTAGCTGCTATTTATGTTCAGACTTGTTCAAAGTGAAACAAAGTTAGTGATCTGTAATGTTTAGCTTCCTATGTCATGAAATAAAGCACAGTTGACCTG


gi|124676893|gb|EH771633.1|EH771633 CNSM4223.b1_N24.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSM4223, 

GTTCGGTTCGGTTCGGGTCAT
||||.|.|||||.|..||| |
CAAGTCGAGCCAGGTTCAGAA

EST sequence (targeted fragment marked with yellow):
GTGCGTGGAATTGAGATTTTGTACAACATGGCTTCGCCTGAGGCTGAAATGGCGTGGTTTTTGGCCCGGACTTCGATTGATTAAGAAGCTTCTTTTGAGCCATCGGGAGGTGGAACTCCTGGGGGGGAGGGATAGCGAAGCTATGTCTACGCATATGGCCTTTGAGCTACCCTGGGAGAGGCAAAGGAGGACAACTTGGTGTTGCACGTTTGGAGCAAGAGGTGTTGCCAGCACAAGACTTGGACCGAGCTGAACACCGCTAACTTTTTCTTGAGGAATTGTCAGAGGCCCTCTGGATTAGATTGATGCCTGGGCTCCCATGGGTCCGAAAGGCAAAGTTAGGGATAAAGCTGGAAAGCTTTTGACCAGGGAGCTTACTGATAGGCCTGTTTTCGAAGCGGAGGATCCATCTGCTATGGGTTGGTCGGTGGCTTCGCGGAACGATAGGTGGGCCACTAAGGTTTACTTA


gi|124681841|gb|EH776581.1|EH776581 CNSM8880.b1_O11.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSM8880, 

GTTCGGTTCGG-TTCGGGTCAT
||||||||||| |.|||.|||.
CAAGCCAAGCCTAGGCCTAGTG

EST sequence (targeted fragment marked with yellow):
CGGGGAAGCTTGAACCCAACAATGGCAGCTCCAAGGTTTCACCTGAAGCAGCAAGGTCACACACCGGAAAATCGAGCTCCGATTCTTCTTCATCTCGTTCCTCTTCAGGTATTTTGAAGAAACTGCTACTGGTCTTCTTCATTGGTTTTCTGGCATTTGCTTATCAATCAGCTCGTCCACCGCCTCCGAAAACCGTTGGATCTCCGGATGGAATTCCGGTTACATCACCGAGAATCAAGCTGCGCGATGGAAGACATTTGTCTTATAATGAATACGGTGTACCAAAAGAGTCAGCCAAATACAAGATAGTTTTTGTCCATGGCTTTGATTCTGTCAAGCATTATGCAGCTATTGCCACATCAGCTTCTCCGGCTCTTATTGAAGATTTGGGGATATATATTGTTTCATTTGATCGTCCGGGGTATGGCGAGAGTGATCCGGATCCGAACCGAACTTTGAAGAGTTCGGCTTTGGATATAGAGGAGCTTGCTGACCAGCTGGGACTTGGATCCAAATTCCATGTGGTTGGTTTCTCTTTGGGTGGGCAAGCAATATGGCCTTGCCTGAAATATATTCCTCACAGGTTAGCAGGGGCGGCTCTTCTTGCTCCGGTCGTTAATTATTGGTGGCCTCGCTTGCCAGCGAACTTATCTAAAGAAGCATTTTCGACGCAGTTCGTTCAGGATCAATGGAGCCTTCGTGTCGCTCACTATCTTCCATGGCTGACCTACTGGTGGAACACGCAGAAACTGTTTCCTTCGCTCACCA