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mature miRNA: GTTCGGTTTGGTTCGGTTCTG
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|225913293|gb|GO365129.1|GO365129 GQ03510.B7P2_K20 GQ035 - Needles - End of season Picea glauca cDNA clone GQ03510_K20 5', mRN

GTTCGGTTTGGTTCGGTTCTG
||||||.|||||. |.||||
CAAGCCGAACCAGACTAAGAA

EST sequence (targeted fragment marked with yellow):
TCACTGTATGAGTGCTTGAGAAATTGGAGTTGATGCAACTGACAGTGGAAACCCTAAAGGCAACTGAGATTGGGAAACAAGTTAATCTGTTGCGGAAGCACAATTCAAAGGAAATTCGGTCTACGGTGAAGAGACTTATCAGTGGCTGGAAAGAACTTGTAGATGAATGGGTGAAGACTGCTGGTAATGTCGCTGTAGCTGCAATTGCAGGTACCTCGCCAGATTCTGTTTCACTTGCAACTGCAGATGATGAAAATGGACACCCTTCGCCCCCACTTGATGAGGGTGCCTTTCTGGCAACCCAGACAACTTCGATAGAAATATCTCAGATCTTCGATGGCATGGATGACGATGGGAATCCTCGTGTTGCTGAAAAGACTGAAAATGATGAATATGATGACGACGACGACGACGATGATGAAAAACAATGTATAGTAGAAAGACCCCATAAAATAAAGCAATCAGCTCATCAGAGTAACCTGAGTAGGGATAGCAGTCCTTGTGGCAAGCAAGAAACAAACAGAGAGAGAGGGGGATTGAAAAGGAATGTTCAAATTCAGGCTAGCAGGGACTGCCGGGGCCATAGGATGAGCAGCTCAAGTCGTATAATTACCAGTTCTGGGCCTGGGAGACCTCCAATAAGTTCGGAATGTAGAGTATATCGAGACAATGTGCAGAAACATACAGATTTGAACAAGAATCAGACCAAGCCGAACTCCTTTTCTCTCCAAAATCATAAATCAAAATCTCCAGATGATGCATCTGTT



gi|255780535|gb|GR954583.1|GR954583 GQ03510.B7P4_K20 GQ035 - Needles - End of season Picea glauca cDNA clone GQ03510_K20 5', mRN

GTTCGGTTTGGTTCGGTTCTG
||||||.|||||. |.||||
CAAGCCGAACCAGACTAAGAA

EST sequence (targeted fragment marked with yellow):
GTATATCGAGACATGTGCAGAAACATACAGATTTGAACAAGAATCAGACCAAGCCGAACTCCTTTTCTCTCCAAAATCATAAATCAAAATCTCCAGATGATGCATCTGTTAAAGTGAAGCTTGAAGCAGCAAAGAGAAGGCTTCAGGAAGGATATCAACAAGTGGAAAATGCCAAGAAGCAGCGCACGGTTCAGGTAATGGAATTGCAAGACCTACCCAAACAAGGCCCTAAACCGTTTCATGGTAGACCAGTGTCACAACATCATCGTCAGTGGATGAATAACCGAAGACAGTGACACCATATTATAAGGCAAAGTTGTCAAGACTCAAGAGGCAGCCACAGGAGTGTAAAAAGCTCTGATGATGAGAAAGTAACCAGCTAGTGTGCTGGCCTGATTTCCATTGTATAGTTTGTGGTCAATCTGGATCTATCTAGAAATTAATTCTAGATTGATTAGAACTCTCACATTAGAAGCTCAGAGATTGAGAGGACAAAACTAAGTTTTGTTTGGGATTCTTTCCTCTCCCATTTTTGTTGATTTGAAGTATCTGTCCTATGTGGCTAGCCTGAGAGTATGGCACATCATAAAGTAAGGAAGCAGAAAAGTCATTTCATGTTTTGATGTGCTGTTTGTATCCAGACAGATGACATTGGCAGCTTTGGCAGGGATTTTGGATCTTCTTAGTTTCATTGCTTGACAAGCTGTAGATGTTGCCAAATCGACTTTCTAATAAATATTGGAGGTCGTATTTACACTGACTCTAATGTATGACATGCAAGGATACAGACTTGATACAATGAATTATCTCTCTACATTTCTATGTTG