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mature miRNA: GTTGAATTGAAGGGAGAAATA
species: Barnadesia spinosa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211684585|gb|GE541660.1|GE541660 CCHS28043.b1_E03.ab1 CCHS Espina Barnadesia spinosa cDNA clone CCHS28043, mRNA sequence 

GTTGAATTGAAGGGAGAAATA
.||||||||||.|  ||||.|
TAACTTAACTTTCAACTTTGT

EST sequence (targeted fragment marked with yellow):
ATATGCTTTGGAATTCGGCTATTACGGCCGGGGTCGGAATCACTATTTTGTTCCTATCCGATTCGATTTGTTTCGAATTTCAACGGAATTCCTGATCGGCGTGGACTACTTGATATCTAAAAGGTGTTCGTCAGAGTCCCTCTTTTGTTCCGATCAGATTTAAATTGTTTCAACTTTCAACTGAATTCTTAATCGGCGCTGCAACAGTACAGGTGTTCGTCGGAATCACTATTTTGATCAGATCGAATTCGAGTTGTTTCAACTTTCAATTCAATTCCTGATTGGCACGGAGTACTTTAATATTTAAAGGTGCTCATCGCATTCACTATTTTGTTCGGATCCGATTTTATCTGATCTGATCGGCTTGGACTACTTTGATATTTAAAGGTGTTCGTCAGATTCACTATTTTGTTCTGATCCGATTTGTCTTGATTTTCAACTGAATTCCTGATCGTTCCCTGAATTTATATTCCTGCCGCTGTTACTGTTCCATCGATCACCATTCACTTAGCCAAATTGAGGTGAATCACTAAATCTCAAGTATAGATTGAATTTTGCTGAGCTTTCGAACAACACAAGAACATGACACCACCAAACAATCATTCTCGGAGCAATAAGGAGTCCAATAGCAATTCAATTCCCAGTCCATAAACCCGCTGTATCATCCATTTTGCGAGCGATCCCCCTTTGCTGCCCTCGATATATTCATGCTAATTCTCGTACTAGGAGCACTA


gi|211676456|gb|GE534481.1|GE534481 CCHS21100.b1_H20.ab1 CCHS Espina Barnadesia spinosa cDNA clone CCHS21100, mRNA sequence 

GTTGAATTGAAGGGAGAAATA
 ||||||||||. |||||| |
GAACTTAACTTTGCTCTTTTT

EST sequence (targeted fragment marked with yellow):
CTTTTTTCTCGTTTCAATTCAAGGGAAAAAGGTCTAGTCATGGAGGCATATAGTTAACTCTAGGCGGTGTTAACGTGGAGGTTTGATTTTTGTTTATTTTTCCTCTAAAAACATCCGCTATATATATTTCTTTAATTAATTACTAAATTTGTTTTCATTTTATTGATCTATCGGCTATGATTAAATTGATTTATCCGCGCTTCTGAGATTAATCTATGAAGTAAAGGATATAAAATGGTTGAATTTCTTGATATGAGCTGATCTTTTCATATTTTCGAGATTTGTATTTGGGTTTTTTCGGTTAATTGGTTGAAAATTGGCATGATGATTGAATTACACAGCTTTGAGCCAGTTCTGCTTCTGAAAATTTATGATTGA


gi|211659599|gb|GE524696.1|GE524696 CCHS11790.b1_K20.ab1 CCHS Espina Barnadesia spinosa cDNA clone CCHS11790, mRNA sequence 

GTTGAATTGAAGGGAGAAATA
.|||||||||||.||.|| |
TAACTTAACTTCTCTTTTGAG

EST sequence (targeted fragment marked with yellow):
CGTTCTTTTCTCCCTAAATTGCAAAACCTCAGCACACTCCAACACCACAGCACAGTTTGTGTTAAGAGGTCTAATCTCTTGTTGGCTCTCAGCCTGTGCGAGTTTTCTCTTCAATTCAATCGCCCTCACAACAATCGTGAAGAAAGATGAATGAAGGGTTTGGAAATAGCGATTTGGCTAAGCTTCAAGCCACTATGCAAGCAGTCGAAATTGCTTGCAGTTCCTTTCAGATCAATGTAAATCCATCAGCTGCTGAGGCAACTTTATTAGCCCTATGCCAGTCCCATACGCCTTACCAGGCATGCCAGTTTATTATTGAAAATTCTCAGTTAGCAAATGCAAGATTTCAGGCAGCAACTGCAATTCGAGATGCAGCTATCCATGAATGGTCATTCCTCTCACCCGATGAGAAAAAAAACCTAATAAGGTGCCGCACTGTCCACAATGTCTATCTCTACAAGACCTTTCGTTCAAATTTGAGATGGTTCCGACTTTTTTGGCTGGATGTGCTGGACCAGTAAAGATAAATTTCAATCTTTGTAATGTCTTTTGTATGCCCATCTTCGCTAACGACCGTTCATGTATAAGTAAGAAGTCTACAAGTATGAGTTGGTCTGAAACACT