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mature miRNA: TACTACAGTGTCTCTCCACAG
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|119010459|gb|DV448497.1|DV448497 CV01024B2F12.f1 CV01-normalized library Manihot esculenta cDNA clone CV01024B2F12.f1, mRNA s

TACTACAGTGTCTCTCCACAG
|||||||||||||||||||||
ATGATGTCACAGAGAGGTGTC

EST sequence (targeted fragment marked with yellow):
GTTTTNNNTTCATGGATAGGAATGGCTCTGTGGAGAGACACTGTAGTATATGGATACAACTCGCCTCTGACATCCTTGAAAGAGTTGGCTTTTGGCTCAACTTGAAGTTCAAAGCAATGTAGCCATGGAGACTAGATGACAGAAAACAACCCGATCCCCAGATCTTAATTCTGAAGCATCAAATGTGAAAATTCACCTGCATTACTGATGTGCCATTTCTTGGTCAAGATTGCAGCCGCTGTTTCGAACCGCGGAAATTTCAATCGGAAAGCTTGTTGTTTGGAGATCACCTGATGCACCGGTGTCCTATGAACATTATTAGTCCTAATCAATATGGTAAGAGAAAACTAGGATGGGGATATAATTAATATTTGTAATACCTGGAGGTGGTATTCTTAATACTCGATTGTTGAGGGGCTCATTGAAGTTGCAGACAGCAAAATAGTTCCTGTTCAGGAATGAATTATTTTATTTGCAATTAAAGGTCGAATTCATTTCAAAAAAAAAAAAAAAAAATCG



gi|164399810|gb|DB946813.1|DB946813 DB946813 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_026_A1

TACTACAGTGTCTCTCCACAG
|||||||||||||||||||||
ATGATGTCACAGAGAGGTGTC

EST sequence (targeted fragment marked with yellow):
TGTGGTTCACTGGGTCAAGTTGGGCTGCAAGAGTTTATGGGAATGGGAATATTTTATGCATGTACTGTTTCAACAGTATCAGCAGAGAATACTTTCTTGCAGCCACTGTTGTTTTGGTTTCATGGATAGGAATGGCTCTGTGGAGAGACACTGTAGTATATGGATACAACTCGCCTCTGACATCCTTGAAAGAGTTGGCTTTTGGCTCAACTTGAAGTTCAAAGCAATGTAGCCATGGAGACTAGATGACAGAAAACAACCCGATCCCCAGATCTTAATTCTGAAGCATCAAATGTGAAAATTCACCTGCATTACTGATGTGCCATTTCTTGGTCAAGATTGCAGCCGCTGTTTCGAACCGCGGAAATTTCAATCGGAAAGCTTGTTGTTTGGAGATCACCTGATGCACCGGTGTCCTATGAACTTTATTAGTCCTAATCAATATGGTAAGAGAAAACTAGGATGGGGATATAATTAATATTTGTAATACCTGGAGGTGGTATTCTTAATACTCGATTGTTGAGGGGCTCATTGAAGTTGCAGACAGCAAAATAGTTCCTGTTCAGGAATGAATTATTTTATTTGCAATAAAAGGTCGAATTCATTTCAGTG



gi|119002396|gb|DV441222.1|DV441222 CV01003B1H09.f1 CV01-normalized library Manihot esculenta cDNA clone CV01003B1H09.f1, mRNA s

TACTACAGTGTCTCTCCACAG
|||||| |||| ||||||||.
ATGATGACACA-AGAGGTGTT

EST sequence (targeted fragment marked with yellow):
GGGACAGAACTGATTGAGAAGGATCCACTTGTTGTGGTGAAGGATAAATTGGGGGAGCTAATGCAGGATGGACTTCATGAGTATAATCAAAGGCACCGTTTACTCAATCTGGAAAAAATGAAGAAGAAAGGAATAGGTTCTGTAAGGTCAGAGAAAAGGAAGGATAATAGCATTAAAGTGTCAGAACAAGATGAATGTGATAGTGGGGATCTAGGGGGTTTGTTCAACCCTGAAGAAAGAAAACGCTTTTATAAAGCTCTTCTGGATGATGATGCCCCATGAAATAAATCATATATTTATTATTATTATTTTGACCATGAAATTTTTCACCCTTTCTGATTGCTATTTCAGGTCATTCGCTATTAGTAATCTGCATTCAGAATGACTGTTAGCTGGTAGTTTTCATATCCAATAATTGTGGAGAACACAGTAGTAGTTGTGTATGTAAACGTTATTGTATAAAAGTCTCAACCAAAGATCTGAACAGCTCAAGTTGCTTCAAGCCTCTCAAGAATGGAATGTTCTGTCAATGTCTTAAAGAAAATGGCAATTGTAATTTGTATTAAAAAACAAAAAAAAAAAAAAAAAATCG