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mature miRNA: TCAGCCAAGGATGACTTGCCG
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|73884445|gb|DT487183.1|DT487183 WS02533.B21_A01 PT-MB-N-A-15 Populus trichocarpa cDNA clone WS02533_A01 3', mRNA sequence

TCAGCCAAGGATGACTTGCCG
. |||||||||||| |||||
GTTCGGTTCCTACTTAACGGA

EST sequence (targeted fragment marked with yellow):
CATGCTTTTATTCACCCCCATTGGGGTTCTCATATGGGGGGGAGGACAGCTGGACGAGTCCCCCTCCCTGTTGATTTTGCAGATGATGGCCCAATTTATGTTAATGCTAAACAGTCCCGTGGAATTATCAGAAGGAGACAATCCCGGGCAAAGCTTGAGGCTCAAAACAAACTGGTAAAAAATCGAAAGCCATACCTTCACGAGTCTGGGCACATTCATCCCTTAAATAGGGTTCGAGGATTTGGGGGACGCTTTTTCAGCAAAAAAAAGCTCCAGGAATCTGATCCCCCTCCTAGTCAGGGTAATGTCCCAGATCCCATCCCCTCCCCCGTAAAAAATGATGCATCAGAACTAGAAAGCTATCAGTCTGGAACCGGACAATTTGGTGCCTCAAATACTCCCTGCTCTGACATCCCCAGTGTTTCCTACACCAATGTCACTTTTAGGCACCCAAAACCCCGGTTTTCAGGTATTGCTACTCCCCTTGGGGGAGGCATGGAAATCAACAGTAGGCTTATTTGCAGAGGAACCCAACCCCCCCCTTCCGTTGTTCAGGGAAAATATGGAAGAAGCCCGGGTAATCGGTCAGGCAATTCATCCTTGGCTTGGTTTTTTTTACTAAAAACAAAATGTCCCCGACGGCCCCTGTTATGTATTTGATCTTTCTGGGGTTTGCTTTTTTTTTTTTTTGTTTAAACATCCTCGGGCATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAA