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mature miRNA: TCAGGCTGTGTGGGATCCGG
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|74158771|gb|DT628703.1|DT628703 EST1155273 Subtracted pine embryo library, Lib_B Pinus taeda cDNA clone PIMD105 5' end, mRNA

TCAGGCTGTGTGGGATCCGG
||||||||||||||||||||
AGTCCGACACACCCTAGGCC

EST sequence (targeted fragment marked with yellow):
CAAACTAAAAATCCAAAGATTATACTGGAACAAAAATATGGTTCTAAGCATCCAAAGACTACTGGATCAAAAATATGGTTCTCATCAAAGTATTTTGCCAAAACCAGGTGCGAGATTATATGCAAAAAAAGTAATATTGTAGCAGCAAAAAAATTCGATTTCAGGAAGGAAGCCTGCGTCTTTCAAAAATCCCAGCTGTGTTCATCTACAAAGTTCTGTCCTGCTGCCTTGGAGGTTGAAGGCCTAATTTTAACCCATGAAAACAGGGTTTGTAGGATCCGGTAATGCCGCAAAAACAAATGACACCGCCTCAGGCATTGGCGGAGGAAGTGAGGCCGTCATAAAATCAAATGAAAACGTCAAAGTGGCTGTAGGCATCGGTGGACAATGTGAGGCCTCATCAATACCGCTTCACTTTATCCACCAATGCCTACAGTCACTTTGACGCTGCTATCTTACTCTGTGGCGTTACCGGATCCCACACAGCCTGATTTCATGACTTCTATTAACCAAAACGGATCTGGGTCTGGTTGTCTCTCTCTCCTCCACCGCCTTCATTTATCGCCTATGTTACTGCTACAGGCTCGGTATTCTTCAAGCAATTACGGTTCCCCCGCGT



gi|70977531|gb|DR746267.1|DR746267 RTCU1_35_G07.g1_A029 Roots plus added copper Pinus taeda cDNA clone RTCU1_35_G07_A029 5', mRN

TCAGGCTGTGTGGGATCCGG
|||||| |||||| |||.|
CGTCCGAGACACCCAAGGTC

EST sequence (targeted fragment marked with yellow):
GAGGGGGAGTGGCTCGCGGGCTTCTCTATCTGCACGAGGATTCTCAACTGCGGATTATTCATCGGGACATTAAAGCAAGTAATATTTTGTTGGATGCAAATCTGAATCCTAAGATAGCAGACTTTGGCTTAGCAAGACTATTTCCTGAAGATGAGAGCCACGTCAGCACAAGAGTTGCTGGAACTTACGGGTATATGGCACCAGAGTATGCTATGCAGGGGCAATTATCTGTAAAAGCAGATGTCTATAGCTTTGGCGTTCTCCTCTTGGAACTTGTTACAGGAAGAAAAAACAGTGATTATAATCTGCCACTTGATATGCAGACGCTTTTAGAATGGGCGTGGAGATTATACAAAGGAGGGAATATTGTGGATATGATTGATCCAGCCATAATTGAAATGTGCAACGTGGAGGAAGCATTGAGATGTGTTCATGTTGGTCTTTTATGTACACAAGCTGACTCATCACTTCGTCCATCCATGTCTGTCATCACTTTGATGCTTTCATCTCATTCTGTGACATCGCTGGAACCCACAGAGCCTGCTTTTCTGAGACCTCATGTTTCTGAAAACAGCAAGTCCACCAGTTGTGGCTCTGGGG



gi|61669936|gb|DN612436.1|DN612436 EST965486 Subtracted pine embryo library, Lib_B Pinus taeda cDNA clone PSABF50, mRNA sequence

TCAGGCTGTGTGGGATCCGG
|||||| |||||||| |||
TGTCCGAAACACCCTAAGCC

EST sequence (targeted fragment marked with yellow):
ACCTCTATTCAACTCATGAAAACAGGGTTTGTAGGATCCGGCCATGTGACAGGATCAGATGGCGGCGGCAGGCATCGATGGACGAAGTGAAGCAGCGTCTGCAGGTATCGGTAGCCGAAGACGAAGTGAGACAGTAGGCCTCCCTGCATCCAGCGATGCCTATAACAGCCACTTCGTCCATCAATGTCTACGGCCACTTTGACTCTGCCATCTGATTCTGCGACATTACCGAATCCCACAAAGCCTGTTTCCATAGAGTTCTGTTGCCCAAAACAGATCTGTGCCTTTCCCCGCGT



gi|57884241|gb|CX649412.1|CX649412 COLD1_35_D03.b1_A029 Root cold Pinus taeda cDNA clone COLD1_35_D03_A029 3', mRNA sequence

TCAGGCTGTGTGGGATCCGG
||||||| |||||||||||
CGTCCGACCCACCCTAGGCC

EST sequence (targeted fragment marked with yellow):
TCCTCGTGGGCTTCTCTATCTGCACGAGGATTCCCAACTGCGGATTATTCACCGGGATATTAAAGCAAGCAATATTTTGTTGGATGAAAATCTGAATCCTAAGATAGCAGACTTCGGCTTAGCAAGACTATTTCCTGAAGATGAGAGCCACGTCAGTACAAGAGTTGCTGGAACTTACGGGTATATGGCACCAGAGTATGCTATGCAGGGGCAATTATCTGTAAAAGCAGATGTCTATAGCTTTGGCGTTCTTCTCTTCGAACTTGTTACAGGAAGAAAAAACAGTGATTATAATCTGTCACTTGATATGCAGACGCTTTTAGAATGGGCGTGGAGATTATACAAAGGAGGGAATAGTGTGGATATGATTGATCCAGCCATAATTGAAACGTGCAACGTGGAAGAAGCATTAAGATGCGTTCATGTTGGTCTTTTATGTACACAAGCTGACTCATCACTTCGTCCATCGATGTCTCTCATCACTTTGATGCTTTCATCTCATTCTGTGACATTGCCGGATCCCACCCAGCCTGCTTTTGTGAGATCTCATGTTTCTGAAAACAGCAAGTCCACCAGTTGTGGCTCTGGGGTTTCACAATCTGCTGCATCTCACGCTCCTCCGTCAAACGCCGATGCTTCGATTACAGAGTTGGTAACCAGGTAACGCGCATCTCGGCCTTAAAAAATGGTACGTCTTTCAATGTCGTGACATAAGTGGGTTCAAAGATACGGACGTGCATAATGTGTAATAATATTACTTTAGTATGCAATTGTAATATTAAGTGTGTGCGTTTT



gi|34345614|gb|CF387197.1|CF387197 RTDR1_11_B11.g1_A015 Loblolly pine roots recovering from drought DR1 Pinus taeda cDNA clone R

TCAGGCTGTGTGGGATCCGG
|||.|||||||| |||||
TGTCTGACACACCATAGGCG

EST sequence (targeted fragment marked with yellow):
CTGCAGCAAGCCTATCAGAAAGTTTTCACGATGTGATGCTGGCAGTAGAAGCTGCCGCCGGTACCGCGCCTTCCCCTCCCTCCACACCGCAAGGCCCTTCCCCATCACGCTACGGTCCCTCCATCTCGCAGGGCACATACACGCCGCAGTACTCTCCACTCAAAGCCAAGAGTCGTCACTCTACACCTCTCCGATCTTCCTTCAGATCTCCAGAAAACGGCGCCTATACTGAGCTCCAAGAGGTTCGTTGCTACGTTTGAGCCTCGCGTCGTAAACAACTCCCTGTCAGAAATATAGTCTGCTCCAGCGCGGGTTCATCACCCTTGAGGGTAAATATCAACGTCGTCGAGAAGAGCTCGTGAAACTCAAAGAAGAGCTACGAGTTCAAGCCGAAAGAGAAGCTCTGCACACCAGCGAACTAAGACACAAACTTGAAATCAATGAAGCGGAACTGAGGAGAACCCGTGATGAGTTTGGCAATGTAGCTCATGCTACGCGCGCCGTCAAGGACATCGTAAATCAAGTTATGGGTGAACTTTCGACTCAACTGAAATGCCCAATCTGTTCAAATCTATTGCGGATACCACACAGTCTGTGTTGCTCCCACGTCGCGTGCTGTGTCTGCATCATCGATTCTTTCCAACACCACGGGGTAAAGTGCCCGATCTGTGGGCAACCTTGTACACAACCCCCGAAGCANGAGGCTGTGATCGTTGGCATCTTATCCGTCACATATAAACTCTTTAAGTCGGAGGAGCCCACGTTCACTTCA