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mature miRNA: TCCACCCATCGTCATCATCTC
species: Lactuca serriola
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|22445673|gb|BU011278.1|BU011278 QGJ15M11.yg.ab1 QG_EFGHJ lettuce serriola Lactuca serriola cDNA clone QGJ15M11, mRNA sequence

TCCACCCATCGTCATCATCTC
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GGGTGGGTAGCCGCAATAGAG

EST sequence (targeted fragment marked with yellow):
ATCTGAGTGAAGACATCAAGAGAAGATTCTACAAAAACTGGTGCAAATCAAAAAAGAAAGCTTTCACAAACTACTCAAAGAAATACGAAACCGAAACAGGAAAGAAAGACATTCACTCACAGCTAGAGAAAATGAAAAAGTACTGCAATGTCATCCGTGTTCTCGCCCACACACAAATTCGTAAGATGAAAGGTCTGAAACAGAAAAAGGCTCACCTAATGGAAGTCCAAGTCAATGGTGGTGACGTGGCACAAAAAGTTGACTTTGCATACGGTTTTTTCGAGAAACAAATTCCAATCGATGCTGTGTTTCAAAAAGATGAAATGATTGATATTATTGGCGTGACAAAAGGGAAAGGGTATGAAGGCGTTGTGACCCGTTGGGGCGTGACCCGTTTACCCAGGAAGACCCACCGTGGGCTCCGTAAGGTTGCGTGTATCGGAGCGTGGCACCCTGCTAGGGTTTCCTTCACTGTTGCAAGAGCTGGACAGAATGGGTATCATCACAGAACTGAAATGAATAAGAAGATTTATAAACTTGGGAAAGCTGGACAGGAATCGCACTCTGCAATCACTGAATACGACAGGACTGAGAAGGAGATAACGCCGATGGGTGGGTTTCCGCATTATGGGGTGGTGAAGGATGATTTTTTGATGATAAAGGGATGTTGTGTGGGGCCCAAGAAGAGGGTGGTGACGTTAAGACAGTCTTTGTTGAAACAGACATCGAGGCTTGCTATGGAGGAAATTAAGCTTAAGTTTGTTGACCCGTCATCCAAGTTTGGGCA