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mature miRNA: TCCCTGTCACATCGGATGTTT
species: Saccharum officinarum
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|35981073|gb|CA271491.1|CA271491 SCBGLB2074A04.g LB2 Saccharum hybrid cultivar SP80-3280 cDNA clone SCBGLB2074A04 5', mRNA seq 

TCCCTGTCACATCGGATGTTT
||||.|||||||||.|| |||
AGGGGCAGTGTAGCTTAGAAA

EST sequence (targeted fragment marked with yellow):
AAGATTTGTCTCGTCCATTTCGTCTAAACTGTGTAATTAATTTTATTTTTTTATTTATATTTAATGCTTCGTGCATGTGTTAAAGATTCGATGTGACGGGGAATCTGAAAATTTTAGCAAAATTTTTGGCATCTAAACGGTGCCTCGGTACTCTCCGTGGCTGCCCGGGCTGCTGGTTGCGCCTGATTGTGACGTTTATGCTGCACCTGCACAGCGTTGACTAGCAGGCATGATAATGATTGGAAGCAGCCGCCCCGCCCGCCTTTGTCTTGTTTTCTTTCTTCTCTGTTCTCTCTACACTCTACAGGCGAACGTTGGTTTCAGTTTATACGCGGTTGGATTGAAAGACAGATACCTTGGACTTTGAAGGCTGCGTGCTAGTTCCGTTCATATTGGTTGAGATTTCGGTAGTAAGACTAAGAACGTACTTGTGGTCAGGCTTGCTTTGGTGGGGGGTAAGACATCAGACATGTGCTAGTGCTTCAGTCTGTCGCTGTGGTCGCTAGTGCTTTTTTACCTGAGAATTAAGTTCTTGTCCTACTTATCTGAATATAAGCTTTTCATCGATTATTTTTTCTCAGACCAATTTCTATCAAGTATCAGTCTCCCGTCCTTATTTTCCCCCCCGTTGCTTTACTTGACGCTATTGTTCGGCGGGTTGATCATTTTTAAATAAATACTGAACAATGATTGCCTTATCATG


gi|36020351|gb|CA284042.1|CA284042 SCMCSD1062E04.g SD1 Saccharum hybrid cultivar (mixed) cDNA clone SCMCSD1062E04 5', mRNA seque 

TCCCTGTCACATCGGATGTTT
| |||||||||.|..|||.|.
ATGGACAGTGTGGTTTACGAG

EST sequence (targeted fragment marked with yellow):
CTCATGTTGCTGCAGAAAAAAGGCCAATTCACATGGGACGAGGTGGTGGTGATGGTTCTTCACATGGTCCGGTGCAGAGAATTCACTAAATACGACCCCAAAATCAATCTTGTTGTCTGTACGCGCTGTTGTCTATTCAACATAGCCTTCTTCGACCTCAATAAAGAGTCGGAGGTTGTGCCGCGCCCACCAATTAGCAAGATCCCTGTCTCTGATCTGTGGCGCTTGGAGGACTCAGTCAACGTCATCTCGATCAAGGTTGCTGAGTCCGACGTTGGTTACCCAATCAACGTATACGGCACCGTGCTGGCAAGGGATGAGTATGACTACAGATGTATCTATTTATTCAAGCGTGGCAGGGACAATCCCCAACTCATCACATCACCGGAGGATACGCTAACTTTAACAGGCCCATATCGAGCATTTGGTGTGACAGGTAATATGTTCTTTGAGGTCCATTTGAAGATTAAGAGAGACGGGGCAATCGACAAAGATTTCAACAAAGCTCTGATACAACACAATACCAGCCATCATACATGCGAACCTATGGCTGTTTCACTAGTAAATTGTCTCAGTACAGTGGTGATGGT


gi|36006181|gb|CA279779.1|CA279779 SCCCLV1001D11.g LV1 Saccharum hybrid cultivar (mixed) cDNA clone SCCCLV1001D11 5', mRNA seque 

TCCCTGTCACATCGGATGTTT
||||.|||||||||.|| |||
AGGGGCAGTGTAGCTTAGAAA

EST sequence (targeted fragment marked with yellow):
CGTTTGGATGCCAAAATTTTTGGCAAAACGCTACTGTAGCGTTTTCGTTGTTATTTGGCAAATATTGTCCAATCATGGTCTAATTAGGCTTAAAAGATTCGTCTCGTCCATTTCGTCTAAACTGTATAATTAGTTTTATTTTTTATCTATATTTAATGCTTCATGCATGTGTCTAAAGATTCGATGTGACGGGGAATCTGAAAAATTTTGCAAATTTTCTGGGAACTAAACAGCCCCTCAACACAAAAGCATCATACACTCTCTCTCAGACGCGCATCGTCAACCATGGTTGTCGAGATGAAGGAGAACGGCGGCGTGGCGTTGGCGGCGGGCGAGAAGGCGCCGCAGCTGGGCGTGAAGCGCGGCGAGCCGACGCTGGTGCCGCCGGCGGAGGCGACGCCGACGGGCGAGCAGTACTACCTGTCCAACCTGGACCAGAACATCGCGGTGATCGTGCAGACTGTGTACTGCTACAAGCCCTCGCCGTCTCCCAGCGGCGGCGGCAAGGACGTCGACGT


gi|268803969|gb|DV548986.1|DV548986 SUM01-073-B06-A-045.g Sugarcane mature leaf tissue library Saccharum hybrid cultivar CoS 767 

TCCCTGTCACATCGGATGTTT
  |||||||||||||||||||
TTGGACAGTGTAGCCTACAAA

EST sequence (targeted fragment marked with yellow):
ATTAGCGAAACAAATCTATTAAGCCTAATTAATCCATCATTAGCACATGTTTACTGTGGCAACGTATTGGCAAATCATGGACTAATTTGGTTTAATAGATTCGTCTCACGAAATAGTCTCAAACTGTACAATTAGTCTATATTTAATACTCTAAATTAGTGTCAAACATCCGATGTGACAGGTTGAAAAACTCATAATGTCATAGGTCCAACAACTTGAATTAATTGATTATAAGAGTTTTACACGAGGCTAGGTATATTGATGGAGCTTAAGAGTAAAGCAATCAAAGATGATTATGTGACTAATTCTGATTTACATGCTACTACCGATCCTATAAAGCATTATCGATTGATGTTGTTGCTCCTCTGGAAGATCAGAAGACAATAAATATTATTGGTCTTAGGGTTGATGCCTTACCTTATGTTTGTCATGGACACTCTACTTTCAAGTTAGTGTGGGTATGATTTTCTTCCATCATATGGTGTCTTTGGAAA