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mature miRNA: TCCTCCCTACTCCTCCCATT
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|61668614|gb|DN611114.1|DN611114 EST964164 Subtracted pine embryo library, Lib_B Pinus taeda cDNA clone PSAAX93, mRNA sequence

TCCTCCCTACTCCTCCCATT
|||||||| || ||||||..
AGGAGGGAAGAAGAGGGTGG

EST sequence (targeted fragment marked with yellow):
ACTCAAGTATTTGAGATTAGGAGCTGAGAGGGTTGTTCTCCGCCCGAGAGCAATGTCTGTTCCATGGTGGAGGATGAACGCGCACGCAATAATGTACGTGACTAGAGGGGAGGGGAGAATGGAGGTGGTGGGAGAAGAAGGGAGGAATGTGTTTAATGGGCACGTGAGAGGGGGTCAGTTCATCGTCATTCCCCAGTTCTACGCAGTCATCAGGGAAGCAGGAGACGAAGGGTTGGAGTGGATAACTTTCACAACATCAGACCTTTCATTGCGGTCGTCTCTGGCAGGAAGGCAATCGGTTTTGAAGGCAATGCCGGAGGAAGTGGTGAGAGCCGCTTACAGGATCGACCAAAATGAAGTTGAACAAGTGATGAGGAACAGAGAACGCGACACCTTCATTTTGCCTCCATCATCTACCCGCTCATCCCGTCGATATCAGGCGGACATCATACCAGGTGTCCAGGTGGAATAGAATATTAATATTACCCATGCATTTCACGCACATTCACTGCAACCCTTAATGTGCATAGATATGCAGTCTCGTCCGAATATAGGCCTCCTCACCTCTGCAAATTAAATGTAATCGAAAGAATAATTATAATAATGGATTTACCATCTTTTTGTTCG



gi|61671999|gb|DN614499.1|DN614499 EST967549 Subtracted pine embryo library, Lib_B Pinus taeda cDNA clone PSAC471, mRNA sequence

TCCTCCCTACTCCTCCCATT
|||||||| || ||||||..
AGGAGGGAAGAAGAGGGTGG

EST sequence (targeted fragment marked with yellow):
ACTCAAGTATTTGAGATTAGGAGCTGAGAGGGTTGTTCTCCGCCCGAGAGCAATGTCTGTTCCATGGTGGAGGATGAACGCGCACGCAATAATGTACGTGACTAGAGGGGAGGGGAGAATGGAGGTGGTGGGAGAAGAAGGGAGGAATGTGTTTAA



gi|61666304|gb|DN608804.1|DN608804 EST961854 Subtracted pine embryo library, Lib_B Pinus taeda cDNA clone PSAA452, mRNA sequence

TCCTCCCTACTCCTCCCATT
|| |||||||||||||||||
AGAAGGGATGAGGAGGGTAA

EST sequence (targeted fragment marked with yellow):
ACCAGGAGAATCCTTTCTGCCAGGGCGTGATAGATCTGCATACCCGCATTGCAAGAAGGAAAATCCAACCCCACAAAAAAAAAATACAGAGCCATCTTTGGCCATATGCTTTCAAAGTCCTGCCTGAAATATTTTATCCCATACAGGGTTTTAATAGATCTATGCTATCAAATATTTAAACTCCAACGAGCTCATATCTCCCTTTCCTCCTTGCCTATCATGGAGGTTCGAAGAATCGTATGACTGTGTCATGCGAGCAGATCTGAAGTGCATCAGCAGCAGAGCTACAAGTGCAGATAAAATAGAGATATGTAGAGAAGGCAATAGGATCCAGGTGAAGTTAAAAAAACCTGTAACAATGCCCAGCTCTCAGATAAGATGCAATCAGGAGCGACTGCATTTGTTTTTTCTCCCCTCCCAGATCCAGTGGCCTTGAAGCTACACGGCATCCCTGTAATACCTGCAGGACATAATCTCCATATTCATCTTCATAGACTATATTGCTGTGTTGCGTTGGAAAGGGAAGTGTGGTGAAGGCAAAAGGAATGGGAGGAGTAGGGAAGACTGGAGCAGAGAATTAATGAAGCAGAGTTTCTCCTTAACCTTAGTCTTACCCTATCCTTCCACACCTCAAGACTTCTAACCATAACACCTCCCTTTCTCGCAACCATCCACCTTCCCTGTTTTCACCCCGCGT



gi|60669253|gb|DN462829.1|DN462829 EST958628 Sequencing ESTs from loblolly pine embryos Pinus taeda cDNA clone RPIIR21 5' end, m

TCCTCCCTACTCCTCCCATT
|||||||| || ||||||..
AGGAGGGAAGAAGAGGGTGG

EST sequence (targeted fragment marked with yellow):
GAACTGCGTGTAAGGCACAATGCTGACAACCCGGAGGATGCCGATGTCTACGTAAGAGATGGGGGACGGATGAATATAGTCAACCGCTACAAACTTCCTGTACTCAAGTATTTGAGATTAGGAGCTGAGAGGGTTGTTCTCCGCCCGAGAGCAATGTCTGTTCCATGGTGGAGGATGAACGCGCACGCAATAATGTACGTGACTAGAGGGGAGGGGAGAATGGAGGTGGTGGGAGAAGAAGGGAGGAATGTGTTTAATGGGCGCGTGAGAGAGGGTCAGTTCATCGTCATTCCCCAGTTCTACGCAGTCATCAGGGAAGCAGGAGACGAAGGGTTGGAGTGGATAACTTTCACAACATCAGACCTTTCATTGCGGTCGTCTCTGGCAGGAAGGCAATCGGTTTTGAAGGCAATGCCGGAGGAAGTGGTGAGAGCCGCTTACAGGATCGACCAAAATGAAGTTGAACAAGTGATGAGGAACAGAGAACGCGACACCTTCATTTTGCCTCCATCATCTACCCGCTCATCCCGTCGATATCAGGCGGACATCATACCAGGTGTCCAGGTGGAATAGAATATTAATATTACCCATGCATTTCACGCACATTCACTGCAACCCTTAATGTGCATAGATATGCAGTCTCGTCCGAATATAGGCCTCCTCACCTCTGCAAATTAAATGTAATCGAAAGAATAATTATAATAATGGATTTACCATCTTTTTGTTC



gi|67557783|gb|DR098428.1|DR098428 STRR1_45_A05.g1_A033 Stem Response Resistant Pinus taeda cDNA clone STRR1_45_A05_A033 5', mRN

TCCTCCCTACTCCTCCCATT
.||||||.||| || ||||.
GGGAGGGGTGATGACGGTAG

EST sequence (targeted fragment marked with yellow):
TCTTCCTTCCACGTCGTTTGGCTCAGGCTATTCCATTCTCCTCTCACAAGCTCTCAGTAGCCCTGGAGAAACTCAATATATCCCAAGGATCCAACAAGGCTATTGCTATGAAGCATACGTTAGAAGAATGTGAAAATCCTGCAATTAGTGGGGAGAGCAAATTCTGCGCAACATCGTTGGAATCCATGATTGATTACTCCATATTTACACTAGGGACCAATGATGTGAAAGTGCTGGCTACGAATGTAAGGAAGGGGCAGCAATATACAATAACTGGGTTTCCTTTCCTAAGCAAATCTGGTTCCATGTCCGTGGCCTGTCACAAGCAGAGATATGCATATGCTGTGTACTACTGCCATGAAACTGAGCACACAAGCACCGCACTGGTGTCTCTAAAGGGCGAAGATGGCAGTAGTGGGGAGGGAGTTGCAGTGTGTCATACAGACACGAGTGGGTGGAATCCTCAACACTTGGCGTTCAAGGTGCTTAATGTGAAGCCCGGTGGTGCACCCGTTTGCCACTTTATTCCGAATGATCACATCGTCTGGCTTCCTGCCAACTAACAGCTACTACCTTCCATAATTTTCTTCCTGTCTCTTATGTATGCTCCATCTCCACTCCTGTCTAATACGCATACGTATTTCTGATATAATATAAGATAATGTAGTCTCGCAACTATNGGGCTCGTCCTCGTCTCTGTCTTT



gi|6019807|gb|AW064735.1|AW064735 ST35B07 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST35B07, mRNA sequence

TCCTCCCTACTCCTC-CCATT
|||||||| |||||| |||||
AGGAGGGAGGAGGAGAGGTAA

EST sequence (targeted fragment marked with yellow):
GGCTGCATTTTCTTGCACGCGTGCTTCAGTTAAAACCTCCGTTTTGATTAAGATCCATTTTTTCGCCTCCGGATCCCATTCTTCCTTTGATTTCAATCCAAATCTCCCTTGCAATGGCTGGAGACAATGTTGCCTATGAGGTAGAGGTAGAGGAAGAGGCAGGAAAGATAGAGGAAGAGGCAGGAAAGGCTGTAGCAGAGGAGGAAGATACAGGAGCTCAGGTTGCACCTATTGTTAAACTACAGGAGGTTTCGATNACAAATGGAGAGGAGGAGGGAGGATGTTATGCTTGATATGAAGGCCAAGTTGTATCGGTTTGATAAGGATGGGAATCAGTGGAAAGAGCGTGGAGTTGGAACGGTAAAGCTGTTGAAGCACAAGGAGACTGGGAAGGNCAGATTGGTCATGAGGCAGGCCAAGACCTTGAAAATCTGCGCCAATCATTCAATACTTCCTTCAATCTCAGTTCAAGAGCACAGCGGTAATGAGAAATCCTGTGTCTGGCANGCTCCTGATTCCCGNTGGGGGAANNGAAGGAAAACT