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mature miRNA: TCGATAAACCTCTGCATCCA
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|164395931|gb|DB941587.1|DB941587 DB941587 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_009_K1

TCGATAAACCTCTGCATCCA
||.|||||||| |.||||||
AGTTATTTGGACATGTAGGT

EST sequence (targeted fragment marked with yellow):
TCACAGTGACAATCAGAGAAGAACGACGTATTTTCCTCCATTTTATCACTCGGCTTTGTGCTGTTCTAGGCGGTACATTTGCTTTGACAGGAATGCTAGATAGATGGATGTACAGGTTTATTGAGGCGGTAACAAAACCAAAAACTAGATTCCGATAGAGGTGGAGGCTGTACAAGTAGTACTAACTGGCATGAACATGTCAAGGGAGTTCTGAGCAATGCTTATGAAATTCCAAGTTTTAAATTACAGAAGCTTCTATTTAAAGCATATACTGCTAGTGTCATCTTTAGTAAAGTTCGACTTTCACCTGACCTGAACATTTCATTGTTTAATGGTTTGGTATTACTATATAGCCAATATGAACGTTGTAGCTTTTGGGGTGGATGATGTTTGAAGGTTGTGATGGGTGTTGTACATGAATTAACTTTCTGGGGATACATCATTGATTGATTTTATTTTTTTGACCTTTATAAATTTTGAAGATGGCTATGATAAAGTTCGCAACTTTATTTTGGG



gi|182383554|gb|FF379944.1|FF379944 CASL415TF CASL Manihot esculenta cDNA 5', mRNA sequence

TCGATAAACCTCTGCATCCA
| ||||||||| ||||||||
AACTATTTGGATACGTAGGT

EST sequence (targeted fragment marked with yellow):
GGGCTCTCAGCTAGCTAGTTCTCCTCGCTCATCAGCTCAGAGAAAAATAGCACTCTCATGGCGAGCAAAGAGGAGGTGAACCAACTAGAGCTCTTCGGGTTTTGGGTTAGCCCATATGTGCTCAGAGTAGAGTGGGCACTGAAACTCAAGAGTCTTGATTATGTCTATACAGAACAAGACATCTTTAACAAGAGCTCTTTGCTTCTTCAATTAAATCCTGTCCATAAGAAAGTCCCCGTGCTTGTTCACGACCAAAAAGTAATCGCTGAGTCCCTCGTCATTCTTGAGTACATCGACGAGACATGGAAGCACAAGTACCCATTATTGCCTCAAGATCCTTATGAAAGAGCCCAGGCTCGGTTCTGGGCTAAATTTGCAGAACAGAAGATTTTGGAAGCTGCATGGAATGCCCTGTGCTCCTTGGGTGATGAAAAAGAAAGAGCAATACAATTGACCATTGAAGCGCTGCAACAGATAGAGGGAGAGCTAAAAGGGAAGCAATTCTTTGGAGGAGAACGAATTGGGTATCTGGACATTGCCGCAGGATGGATCTCTTACTGGTTGCCTGTCTGGGAAGAAGTTGGGTCTATGAAGATCGTAGACACCCAGCAATTTCCAGCGATTTCTTCTTGGATGCATAGGTTTATCAATCATCCTGTGATCAAAGACAATTTGCCTCCGCGGGATAAGATGACCGTTTACTTCAACAGTCGCCGTGAAGTACTAACTTCTCAGCCTCAT



gi|119011014|gb|DV449496.1|DV449496 CV01028A1B10.f1 CV01-normalized library Manihot esculenta cDNA clone CV01028A1B10.f1, mRNA s

TCGATAAACCTCTGCATCCA
||||.|||||||..| |||
AGCTGTTTGGAGGTGGTGGT

EST sequence (targeted fragment marked with yellow):
TGGCCAGAAGTGGGGTGATCGTAGACGTGAAATTCTGTTTCCAAGAGGAAATTAACCTCACAGTTCGCAAAATCTCCGTCTTCTTTCCTGGAGACGGCTTCATCGTCTATAATCCTGACGGAGAAGTCATCTTTCGATTTGATTCTTACGGTCCAGATTCCCAGCCTAAGGATGAGCTAGTTCTCATGGACGCTTCTGAAACCGAGTCTTCATCATCGGTGGGAGGGCTTTCTAGGTGAGAGAAAGGTCTGCAATGGTGATCATCAGGAGGCTATATTCTGTGTGTGCAGATCAAGCATCATCGGACGATCCAGCTTGGTGGTGGAGGTTTGTCGAGATCCCCGTGAGGAGTACTGCATAGAGGGTAATTATCCTCAGCGGCGGTGTAAAATTTACAGCGGTGGTGCTTCCTTGGAGAATTCATTAAAGCAAGCGGTGGCAGAGATCAAAAGAAAGGTGGACCCTTCAACTCATATGTTGCTTGGCAAGGATGTGTTTTGGCTGTGTGTTCAGCCAGGGCTTGATGCTGCTTTTGCCATGGGATTAGTGCTTGTTCTTG



gi|164380989|gb|DB935499.1|DB935499 DB935499 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_043_J1

TCGATAAACCTCTGCATCCA
||||.|||||||..| |||
AGCTGTTTGGAGGTGGTGGT

EST sequence (targeted fragment marked with yellow):
GATCTAATGATCTTCTGGCTGCCGCCGCCGAAGATTTGTATGGCCAGAAGTGGGGTGATCGTAGACGTGAAATTCTGTTTCCAAGAGGAAATTAACCTCACAGTTCGCAAAACCTCCGTCTTCTTTCCTGGAGACGGTTTCATCGTCTATAATCCTGACGGAGAAGTCATCTTTCGATTTGATTCTTACGGTCCAGATTCCCAGCCTAAGGATGAGCTAGTTCTCATGGACGCTTCTGGCAAGTGCCTCCTCTCCATCCTTCGAAAGGTTTGTTTGTTTGTTTAGAAACGAAATCTTTTCGATAGCAAAGCTTGCTGTTGAGTTGTGGTTTTTTTTTGGTTTTTGATGAAAATTAAGCAGAAACCGAGTCTTCATCATCGGTGGGAGGGCTTTCTAGGTGAGAGAAAGGTCTGCAATGGTGATCATCAGGAGGCAATATTCAGTGTGTGTAGATCAAGCATCATCGGACGATCCAGCTTGGTGGTGGAGGTTTGTCGAGATCCCCGTGAGGAGTACTGCATAGAGGGTAATTATCCTCAGCGGCGGTGTAAAATTTACAGCGGTGGTGCTTCCTTGGAGAATTCATTAAAGCAA