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mature miRNA: TCGATAAACCTCTGCATCCAG
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|182383554|gb|FF379944.1|FF379944 CASL415TF CASL Manihot esculenta cDNA 5', mRNA sequence

TCGATAAACCTCTGCATCCAG
| ||||||||| ||||||||.
AACTATTTGGATACGTAGGTT

EST sequence (targeted fragment marked with yellow):
GGGCTCTCAGCTAGCTAGTTCTCCTCGCTCATCAGCTCAGAGAAAAATAGCACTCTCATGGCGAGCAAAGAGGAGGTGAACCAACTAGAGCTCTTCGGGTTTTGGGTTAGCCCATATGTGCTCAGAGTAGAGTGGGCACTGAAACTCAAGAGTCTTGATTATGTCTATACAGAACAAGACATCTTTAACAAGAGCTCTTTGCTTCTTCAATTAAATCCTGTCCATAAGAAAGTCCCCGTGCTTGTTCACGACCAAAAAGTAATCGCTGAGTCCCTCGTCATTCTTGAGTACATCGACGAGACATGGAAGCACAAGTACCCATTATTGCCTCAAGATCCTTATGAAAGAGCCCAGGCTCGGTTCTGGGCTAAATTTGCAGAACAGAAGATTTTGGAAGCTGCATGGAATGCCCTGTGCTCCTTGGGTGATGAAAAAGAAAGAGCAATACAATTGACCATTGAAGCGCTGCAACAGATAGAGGGAGAGCTAAAAGGGAAGCAATTCTTTGGAGGAGAACGAATTGGGTATCTGGACATTGCCGCAGGATGGATCTCTTACTGGTTGCCTGTCTGGGAAGAAGTTGGGTCTATGAAGATCGTAGACACCCAGCAATTTCCAGCGATTTCTTCTTGGATGCATAGGTTTATCAATCATCCTGTGATCAAAGACAATTTGCCTCCGCGGGATAAGATGACCGTTTACTTCAACAGTCGCCGTGAAGTACTAACTTCTCAGCCTCAT



gi|164395931|gb|DB941587.1|DB941587 DB941587 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_009_K1

TCGATAAACCTCTGCATCCAG
||.|||||||| |.||||||
AGTTATTTGGACATGTAGGTA

EST sequence (targeted fragment marked with yellow):
TCACAGTGACAATCAGAGAAGAACGACGTATTTTCCTCCATTTTATCACTCGGCTTTGTGCTGTTCTAGGCGGTACATTTGCTTTGACAGGAATGCTAGATAGATGGATGTACAGGTTTATTGAGGCGGTAACAAAACCAAAAACTAGATTCCGATAGAGGTGGAGGCTGTACAAGTAGTACTAACTGGCATGAACATGTCAAGGGAGTTCTGAGCAATGCTTATGAAATTCCAAGTTTTAAATTACAGAAGCTTCTATTTAAAGCATATACTGCTAGTGTCATCTTTAGTAAAGTTCGACTTTCACCTGACCTGAACATTTCATTGTTTAATGGTTTGGTATTACTATATAGCCAATATGAACGTTGTAGCTTTTGGGGTGGATGATGTTTGAAGGTTGTGATGGGTGTTGTACATGAATTAACTTTCTGGGGATACATCATTGATTGATTTTATTTTTTTGACCTTTATAAATTTTGAAGATGGCTATGATAAAGTTCGCAACTTTATTTTGGG