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mature miRNA: TCGATAAACCTCTGCATCCAG
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|9853427|gb|BE598354.1|BE598354 PI1_81_E06.g1_A002 Pathogen induced 1 (PI1) Sorghum bicolor cDNA, mRNA sequence 

TCGATAAACCTCTGCATCCAG
  ||||||||||||||.| ||
CACTATTTGGAGACGTGGTTC

EST sequence (targeted fragment marked with yellow):
GCGTGCCCAAGCTGGTACGTGGGGAAAGCAACTTGGTGCAGAGGTTTATCACTGTAACAACATGGTGGAGCAGGAGCTGCCTGGTGGCGGACTCCTCATCTACCAGAGCTTCTGTGCTGCTGAAGACGCTGTTGCTACCTCGCCCAAATCTGTTCTTCACTGCTTCGAAGGTGTGAACATGGAGAATGCTCCTCCTGTGAAGGACCGCAAACTGGCTAATCTTCTCTGCTGGGAGCAAGTGGATGCCATGGAGAAGGATGGAGTGCTTGATGAGTAAGATGGGCTTCTGGTGTCGATTTGCTTCTGAGTTGTGTTTTTTTATCTTTGCAATTTCGTTGTCGTCGTTTGGTTATTCTGTGAAGCAGCCAAGCCAGGCTATTAGAGTATGAAAAATGTCGTCTGTAAGCATGTTTTCCTATGTTGCCACATGCTGAATCAATTTGAATAAGTATTAATACAGCTTTAGGTGGTCAGCTGCGTCTACCACAATGAGCATATATGTATGGA


gi|9303570|gb|BE362013.1|BE362013 DG1_83_G02.g1_A002 Dark Grown 1 (DG1) Sorghum bicolor cDNA, mRNA sequence 

TCGATAAACCTCTGCATCCAG
 | |||||||||||||.| ||
CGGTATTTGGAGACGTGGTTC

EST sequence (targeted fragment marked with yellow):
TTTTCTGTCGCTGTGACCATCTTCGGCGGGCGTGGCCAAGCTGGGACGTGGGTAAAGCACCTTGGTGCAGAGGTTTATGGCTGTAACAACATGGTGGAGCAGGAGCTGCCTGGTGGCGGACTCCTCATCTACCAGAGCTTCTGTGCTGCTGAAGACGCTGTTGCTACCTCGCCCAAATCTGTTCTTCACTGCTTCGAAGGTGTGAACATGGAGAATGCTCCTCCTGTGAAGGACCGCAAACTGGCTAATCTTCTCTGCTGGGAGGAAGTGGATGCCATGGAGAAGGATGGAGTGCTTGATGAGTAAGATGGGCTTCTGGTGTCGATTTGCTTCTGAGTTGTTTTTTTTATCTTTGCAAGTTCGTTGTCGTCGTTTGGTTATTCTGTGAAGCAGCCAAGCCAGGCTATTAGAGTATGAAAAATGTCGTCTGTAAGCATGTTTTCCCTATGTTGCCACATGCTGAATCAATTTGAATAAGTATTAATACAGCTCTAGGTGGTCAGCTGCGTCTACCACAATGAGCATATATGTATGGAGAAG