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mature miRNA: TCGCTTGGTGCAGTTCGGGAC
species: Raphanus sativus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|161576918|gb|EY948368.1|EY948368 RS3DP33TF RS3(RT) Raphanus sativus cDNA 5', mRNA sequence

TCGCTTGGTGCAGTTCGGGAC
|||||||..|| | ||||.||
AGCGAACTGCGCCTAGCCTTG

EST sequence (targeted fragment marked with yellow):
TTTTTCTTTCTTTCTGTGATTTTCTTAAAGTTTCGTTTCTTCGTCAGGTGAAAATCAAAGAGAAAAAACAAAACTTAAAACGTTCCGATCCGCGTCAAGCGATTTCCGCCATGTCGATGATGACAACTGTCAAAGTGAGCAATGTTTCTCTAGGAGCAACCGATCGTGACCTCAAGGAGTTCTTTTCCTTCTCTGGCGACATTCTCTACCTCGAGACCCTCAGCGAGACAGAACGGTCCAAATTGGCTTATGTCACTTTCAAGGATCTACAAGGAGCTGAAACTGCCGTTCTTCTCTCGGGAGCAACGATTGTTGATTCTTCGGTCATTGTCACTATGGCTCCTGATTACCAACTCTCTCCTGAGGCTTTAGCTTCCTTGGAGCCCAAGGAAAGCAGCAAGTCTCCCCGTGCAGGTGACTCCGTGTTGAGAAAAGCCGAAGATGTTGTCAGCAGCATGCTCGCAAAGG