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mature miRNA: TCGGACCAGGCTTCAATCCCT
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|30973106|gb|CD229672.1|CD229672 CCC1_18_D06.g1_A007 Callus culture/cell suspension Sorghum bicolor cDNA clone CCC1_18_D06_A00 

TCGGACCAGGCTTCAATCCCT
| ||||||||||||| | ||.
AACCTGGTCCGAAGTAAAGGG

EST sequence (targeted fragment marked with yellow):
TACTGGGTGGTGCAGCCAGCTGAGCTACAGGTGCCGGCCAAACTTCCCCAACTGAATGTCTTGCAGGTGACTGAGGAGGCTGAGCTGCAACGCCTCTTCAGAGCCCATGCCATGGCCCTCCAAGGAGCTGTGCAGCGCATCGTGGACCGTGCGGGTGGAGAGACGGCCTCCCTGCTGAAGTCACTCATGTACGACTACATCGACAAAGCCTCTGCGCTAGCTGGCGGGCTCGGGCTGATCCAAGCCCCGCCGGAAGCTGCTACGCCGGGAAATGAAGCCTGGTCCAAGTGGCCACCCCAGCCAGCTCCGACACACCAAGCTACTCACGCCACGGTACCCGTGCGTGACGTCTTCGGCCGCATCCTCAGCACGCTACCACCGAAGGCAACAGTGGAAGACAACTCCATTGCTGCGGTCGAGAAAGCACTGGCGCGCATCGAGCTCTCTGCAAGGGAGAGCCCCACAATTCAGGGTGACAGCAGCGGTGGCCAGCAGCTAACCAGCAGTGTCGTAGCACTCTTCAGCAAGCCGACGGAGCCGCTGCTCCAACAACCTCAGCACGCAGCTGCTGTCGTGCCAACAC


gi|45975730|gb|CN140010.1|CN140010 OX1_33_B02.g1_A002 Oxidatively-stressed leaves and roots Sorghum bicolor cDNA clone OX1_33_B0 

TCGGACCAGGCTTCAATCCCT
.|||||||||||||| ||||
GGCCTGGTCCGAAGT-AGGGT

EST sequence (targeted fragment marked with yellow):
CCACGCGAGCACACCCACCACCTACTCCTACTCCCCATGGCAGGAGCGCGCACGGGAGCCGGCTGCTGCTCCACCTCCTCTCCTCTCCTGCTGCACTACTAGGCGGTGCCCGCCGGCGACGACGATGGCGATGGTCGCGAGCAGGGAGAGGCGGCTCTCCCCGCCGGGCGGCGCCGCCCAGGGCGCGCCGCAGGTGGACGCGGGCAAGTACGTGCGGTACACGCCGGAGCAGGTGGAGGCGCTGGAGCGCGTCTACAGCGAGTGCCCCAAGCCCAGCTCGCTCCGCCGGCAGCAGCTCATCCGCGACTGCCCCATCCTCAGCAACATCGAGCCCAAGCAGATCAAGGTCTGGTTCCAGAACCGCAGATGCCGGGAGAAGCAGCGCAAGGAGTCTTCTCGTCTACAAACCGTGAACCGGAAGCTGAGCGCGATGAATAAGCTGCTGATGGAGGAGAATGACCGGCTGCAGAAGCAGGTGTCCCGTCTCGTCTTTGACAACGGGTATATGAAGAATCGACTCCACAGTCCTTCTGTAGCCACCACCGACACAAGCTGTGAGTCTGTGGTGACAAGTGGTCACCACAACCAACAGCAAAACCCAGCAGTTCTGCATCCTCCACAAAGGGATGCGAACAACCCAGCAGGTCTACTCGCTATTGCTGAGGAGACATTGGCAGAGTTCATGTCCAAGGCGACTGGAACTGCTGTCAACTGGGTGCAAATGGTTGGGATGAAGCCTGGTCCGGATTC


gi|14569631|gb|BI098049.1|BI098049 IP1_31_D07.g1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA, mRNA sequence 

TCGGACCAGGCTTCAATCCCT
| ||||||||||.||||  ||
AACCTGGTCCGAGGTTATCGA

EST sequence (targeted fragment marked with yellow):
GCTGAAAATCAAGCTACTGATGGGAAGCAGAGTGTGTAGAAACCTGAGGCCGTAGGGGAACAAAACGCCGTGGTGGAGAATGGTGTGGAGGCAATAGCTGTTAATGGTGTCGCCCCCAGAAGATATTAAGCCAACAGCTGAAGAGGCGGCCATTGCAAATGGCTGTTAATTTTCACTGCATGCCATCAGCTATTGGAGCCTGGTCCAAGGAGACACCTTTTGTGCCACAGAAGCGTTGGTGTTCAAGTCATTCAAGATTCTGCTATGTTTTTTTCTCTCTTTGTTTTTCCTTTGCAATGATGCTTGGTTGCAGAAGGGTGCAGTCTAAGGCAATTCTGTATCCTGCTAGGTTTCTCGATGCATTGTCATTTAAGGTGGTTAGATGGATATTAGCTGTTCTTATTCCATCGAGTTCTGTCCTTTGTCTTGTCGTTTGGAGTTGTACTTAGGCGTGGAGATGTGAAAGGCCCTGGTTCGTGGGCTCATGGCATTGGATTAGATGAACTGAATTGGAGCTCCAATTCAAACACATGTGGAAATAGAAATGAGGTCCAGTTGTGGATTTGAAGCG