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mature miRNA: TCGGACCAGGCTTCATTCC
species: Cycas rumphii
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|158977583|gb|EX929218.1|EX929218 ll22c03.g7 Cycas ovule - normalized (NYBG) Cycas rumphii cDNA 3', mRNA sequence 

TCGGACCAGGCTTCATTCC
|||||| ||||||||||
AGCCTGCTCCGAAGTAATA

EST sequence (targeted fragment marked with yellow):
TGTTTGAGGGTGGGTCTTAAGTGAGGAAGCTTTTGAAGTTTCCAAGTTATCTGCTAGCTTCAAGTATTATTCAAGTTGAAAGATGGCTGCCATACATATGCTTAATGCATGTACCATAAGGCTAACAAGGCAATCAAATTATCAAGAATGGAAAAGAATCACAAAAAATCTCTTGATAATGAATGAGTTCTGGGGATATGTTTCTGGGAGAATCCCCACTCCTACACTTGTGGTTAATAATAATCAACCTGCACCTATGGATGTTCAACTTGTTGCTGCATGGGAGGCCAAAGACGAAATTGCTTTGGCATTCATTCGAATGGTTGTGGACATACATGTAGACCAAAATATTTCTTCTGCACTATCTTCTAAAGAAGCATGGGACACCTTGTCCTCTTTGTATGAAAGGCGAGATGAAGCTCGCATTTTGATGATAAAAGCTAAATTGTACTCCATCAAATTTGTAAAGCCAATGTCGGCATTCATGTCCTCTATTAAGGAGGTTTGTAATGAATTAACTACTTTAGGTAGTCCAGCTACAAGAGATGAAATCGTAGCCTCCATCACAAAAGCATTGCCAAAGGACTACAACGGCTTTATATCATCAACTACACGAGATGGTTCACTAAGGGGGATGACATTCAATCAACTTGAAAGCTTATTGCTTCAAGAAGAACAAATATATGCTAAGGATAATGAAGCCTCGTCCGAATTCTTTGGATCCA


gi|158977066|gb|EX928532.1|EX928532 ll14b11.g7 Cycas ovule - normalized (NYBG) Cycas rumphii cDNA 3', mRNA sequence 

TCGGACCAGGCTTCATTCC
|||||||||||||||||||
AGCCTGGTCCGAAGTAAGG

EST sequence (targeted fragment marked with yellow):
GATAGAGGGCCGGATTAATTCAGTCGACAGACGGGAAATCCAGCCTGCAAAAAGTAGAGTAGAGCAGGTGCACCCTGCAATGATGTGAGATGGATACATATGCATCACATGACCCACCCCGGGCGGAAACGACTGAACGAACGATAAAAATCCGGCCATGGCCAAACGATCGACCCCGTAAACCTTCAATTGCAGGAAGGACGGTTCATCAAAATAGGTGTTTTAATATCTGAGCAAAGCTCATGTGACCGGAAGCACGCATCTCCACGTCATATGCCGAAGAAAGCCATGGAGAGGAGCTACCCTGAAAAATAAAGGAAAACGGGCGCACAGATCGACCAGAACACGCTTGCGCAGAGCCTTTGGGTTGGCAAGGGATCGCCATGGACGATCGACCTCTTGCTACAGGGCGATTTCACAAACACGGAACCCTCCAAAACAATCAACCAAATCCCTCGGGCCCCTGAACCAGAACTCGACGAATTATACGAAGCATGGGGAGATGAGATTCGTCCCGGAGGGAAGTTCAAATTGGGGGGAATGAAGCCTGGTCCGACAGCACCCTCCAACCAAAAGTCTGTGGATCGAGTCGAGCCAGACAACATTCCCCTCAACATTGAGATCTTCCTGCTAATCTTCCATCCCATTGCATTCCTTGCGTTCGACCATTCGTTTTTCGGTAAAACC