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mature miRNA: TCGGACCAGGCTTCATTCCCC
species: Brachypodium distachyon
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|270437748|gb|GT821524.1|GT821524 CCYO6535.g1 CCYO Brachypodium distachyon flower + flower drought Brachypodium distachyon cDN 

TCGGACCAGGCTTCATTCCCC
||.||||||||.| |.||| |
AGTCTGGTCCGGACTGAGGTG

EST sequence (targeted fragment marked with yellow):
AGAGCTTGAATTACAACAGTGAAGAGGAAAAGGCTGCTGCTGTTGAAGCCTGGGTTGCTTGCCATGGCAAGGAACCGAAGTCCGTGATTGGTCTGCAAGCATCTGAGATCTCATGGGATCACGAAGGCGAGCACCACTATTACAAGGCAGGGGTCCCTTGGTGGCGGCAAGATTCAGTGGAGTCAGGCCTGGTCTGAACTGTGACAGCCATACAAACGAGAATTCCAGCTCCGGAGCACTGTCCCCCGAAACCGAAGTGACCGGTGCGAGTACCCTCATCTGGGGGAGAGGGGATATCTTACTTGTCTGACAAAGAGAAGCATGGCTGATACACCAACACCGATTTTGGGCCGGAACGCCTCGCCGACCGTCAGCACAAATCTAACACGCCGAAGGTATTCAGATAGAATGTCTGATGCTGGACCTGTAACTGTTGTAATCGGGGCAGAGTTGCTCTGCCAATTCAATTTTACCAGGGTTTACCTGGTTGCTGTAGTTTCTAAGATTTCACCTGTCTGTGTACCTCGTACTAAACTGTCCCTGGTCCTTGTGTACTCTGCGTCGAGGTTGAAAGGAAATTTGGTCCCCGTAC


gi|270379807|gb|GT763625.1|GT763625 CCXF4427.b1 CCXF Brachypodium distachyon abiotic + biotic stress pool Brachypodium distachyo 

TCGGACCAGGCTTCATTCCCC
.|||||||||||||||.||
GGCCTGGTCCGAAGTAGGGTC

EST sequence (targeted fragment marked with yellow):
GGCTACAGGGACTGCTGTTGATTGGGTCCCGATGCCTGGGATGAAGCCTGGTCCGGATTCGTTTGGTATTGTGGCCATTTCACATGGTTGCCGTGGTGTTGCTGCCCGTGCATGTGGTTTGGTGAATCTAGAACCGACAAAGATTGTGGAGATCTTGAAAGACCGCCCATCTTGGTTCCGTGATTGTCGGAGTCTTGAAGTCTTTACACAGCTCCCAGCTGGAAACGGCGGGACTATTGAGCTTGTTTATATGCAGATGTATGCTCCTACTACTCTAGTTCCTGCACGGGATTTTTGGACACTGAGATACACAATAACAATGGAAGATGGCAGTCTCGTGGTTTGTGAGAGATCTTTGAGTGGTTCAGGAGGTGGTCCAAGTACTGCCTCAGCACAGCAATTTGTAAGGGCTGAGATGCTTCCTAGTGGCTATTTAGTTCGGCCATGTGATGGCGGGGGTTCAATTGTGCATATGGTGGACCATCTGGACCTTGAGGCTTGGAGTGTTCCAGAAGTCCTCAGACCACTCTATGAGTCGTCTAGGGTTGTTGCCCAGAAAATGACTACTGCGGCATTACGACACATCAGACAAATTGCTCAAGAAACTAGCGGGGAGGTTGTATATGCTTTGGGGAGGCAGCCTGCTGTCCTACGGACATTTAGTCAGAGGTTGAGTAGAGGATTTAATGATGCTATAGTGGTTTTAATGATGATGCTGGTCTGTCATGGA