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mature miRNA: TCGGACCAGGCTTCATTCCTC
species: Vitis vinifera
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|110374378|gb|EC939091.1|EC939091 WIN058.C21_J05 Cab Sauv flower, leaf and root normalized (WIN05) Vitis vinifera cDNA clone W

TCGGACCAGGCTTCATTCCTC
.|||||||||||.|||.||.
GGCCTGGTCCGAGGTAGGGGA

EST sequence (targeted fragment marked with yellow):
CAAAGCCCCAGTTTCCCTGTTCCCACCTTCCACCATCAAGCTAGAATTCAGATCAAAACCAGCCTTAGCAGGCTTAACTCCATTCACATGAAATGGTTGGGAATTCATGCCCATTAGGAATGGCTGCGGAGAGCATGAAGGAGGAAAGGTCACTGCAGAACCCATTATCTGCGGTACTATAGGAGCTCCTCTTGAATCTATCATGTAGGGGATGGAGCCTGGTCCGGACACAGTGGAGAGAGTCATAGAGGGTCCCATTATAAATCCACTGTACCCAAATGGCGAGGATGCACAAGCCACTGGATGCTGAAACTCCATGACACCTTGAGGCCTTGCCAAGTTGGTGCCCCTTGATGAATAGCCAACCTGCCCATTTAGCAGAAGAGACTGGGATGGTGTGAAGTTCCTTCCATTAACTGGTGCCCTAGAGCCAATAATCAGAACATCAGAATCCTCCTGCTTAAACCCTACAGAAGCACTCATTTCTTTCGAAGATGATTGCCCAAGATCATTCCGTTGGTCATACATATCATTAGTGAAAGAAAGATTTTCATTCAAGTCAATGTTAATCATTGAAGGTTGCCTAGATGATGATGAGGCAGCATGTGAGGGACTACGATGCTCATCCAGATTATGATAAAAGGAGTCCACCTCCCTCCTATGCGATGACGGAGCATCATCATCATCACCAACACGATTCAGGTCCAAATTAAGCCTCTCTGCCCGCTTTGAGTTCACTTCAACCAAAGACTCACCAGAAATTATGCCAGATGAAGCAGGGATCTGATCCGTAATTATTGTATCAGCTGCTCCATCAACACCATCATCAGCCACATTCAGATCAATCTCAAGCAAGTCCTGCCTCTGCTTCAAACTGTAGCTGCTTCCCTCAGCAGATACCGTCTT