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mature miRNA: TCGTGCCAAGCTGTGTGCATC
species: Pinus contorta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|262148918|gb|GT257805.1|GT257805 PCO021.C21_A10 Pco02-Xylem-wounded-Norm (PCO02) Pinus contorta cDNA clone PCO021_A10 5', mRN 

TCGTGCCAAGCTGTGTGCATC
|||||||| || || ||||||
AGCACGGTCCGTCAGACGTAG

EST sequence (targeted fragment marked with yellow):
GGGGATCCTATGCTTATTCTTTCCTTTCCACCGAATAATCCTTCTGAATTTTTTGAGTAACTTCTTTGGATGCAGACTGCCTGGCACGATTCGTACTCTGGACGACTCGTGCCAAGCTGTGTGCATCTCAAGATCAGAAGAGGCTCTCGTTCTGCATTTCTCTGCTCAAAATTTTGGGTTTGTGCTGGCTAGAGAAGCTCCATGCTCAAAGTTTCAATAATTCTGGTAGAGATCCATTAAGAATATCTTGTGAAAAGTGAAATCACAGAATTGTGGGTTCCCTGGGTAATACTCAGAATTGCAGATTGAAATTGTGTACACAAGGAACACAGAGCCGACTTAAATTGCAAGCTGTGCCAATTTGAAGCAGCCTGGTGAACAACTCCCATATCTATTATTCAATTTTTCTCTCTGGAAAACTGTTGTACAGACTATGGGCATACGCTTTTCAGCAGGCATAGCGGGCAAATTTCCATACCTATTATTCAATAAGTTGTCTGAGCAATCATTATACAAATAACTGTTGTACAGACGATGGACATACGCTTTTCAGTAAGCAAAGCAGGCAAATTATAATAACTGTTGTACAGACGATGGGCATACGCTTTTCAGTAAGCAAAGCAGGCAAATTATAATAACTGTTGTACAGACGATGGGCATACGCTTTACAGTAAGCAGAGCANGGAAATTTCAAAGTCAAAAAAAAAAAAAAAAA


gi|262147634|gb|GT258171.1|GT258171 PCO021.CR_A10 Pco02-Xylem-wounded-Norm (PCO02) Pinus contorta cDNA clone PCO021_A10 3', mRNA 

TCGTGCCAAGCTGTGTGCATC
|||||||||||||||||||||
AGCACGGTTCGACACACGTAG

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTTGACTTTGAAATTTCCCTGCTCTGCTTACTGTAAAGCGTATGCCCATCGTCTGTACAACAGTTATTATAATTTGCCTGCTTTGCTTACTGAAAAGCGTATGCCCATCGTCTGTACAACAGTTATTATAATTTGCCTGCTTTGCTTACTGAAAAGCGTATGTCCATCGTCTGTACAACAGTTATTTGTATAATGATTGCTCAGACAACTTATTGAATAATAGGTATGGAAATTTGCCCGCTATGCCTGCTGAAAAGCGTATGCCCATAGTCTGTACAACAGTTTTCCAGAGAGAAAAATTGAATAATAGATATGGGAGTTGTTCACCAGGCTGCTTCAAATTGGCACAGCTTGCAATTTAAGTCGGCTCTGTGTTCCTTGTGTACACAATTTCAATCTGCAATTCTGAGTATTACCCAGGGAACCCACAATTCTGTGATTTCACTTTTCACAAGATATTCTTAATGGATCTCTACCAGAATTATTGAAACTTTGAGCATGGAGCTTCTCTAGCCAGCACAAACCCAAAATTTTGAGCAGAGAAATGCAGAACGAGAGCCTCTTCTGATCTTGAGATGCACACAGCTTGGCACGAGTCGTCCAGAGTACGAATCGTGCCAGGCAGTCTGCATCCAAAGAAGTTACTCAAAAAATTCAGAAGGATTATTCGGTGGAAAGGAAAGAATAAGCATAGGATCCCC