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mature miRNA: TCGTTTGTTTTTGGCTGCCTT
species: Lactuca sativa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|90499162|gb|DY961020.1|DY961020 CLSM11372.b2_G12.ab1 CLS(LMS) lettuce sativa Lactuca sativa cDNA clone CLSM11372, mRNA sequen 

TCGTTTGTTTTTGGCTGCCTT
|||||||||||.   ||||||
AGCAAACAAAAGAAAACGGAA

EST sequence (targeted fragment marked with yellow):
AGGACTCTCCTCCGATTTCGACAAGAAAGCCCAAGATCTGTTAACGAGAAAGAACCTAAAGGATCAGAAAGTCTCTGTTTTAGCGAGCAGTGTTACGGAATTTAATACTGTAAGATATTCACCAAAGAGACCAGTTGAGAATGATGTTAATGGGATGAATCTAGTGTCAAACACTAGATCCACCTCAGGAGCTAAAAGTCAAACCATTGGAGCCAAAAGGCAAAAGAAAACAAACGAAGGCTTAAAAGATACGGGTTTGCAAATAAATGGTCATCCTCAGACAAATGCTTTGGCTTCTGAAAATGTAGCTACAACCAATTCTAAATTGGAGACTGAGATTGAAAAATCTCTTTCTAGCGGGCAGCAGGATGCGTTGAATTCAAAAAAACGTCCTTGTGCCTTTTGTCATTCTTCTAGTCAGACAGAGGGAAGTGAACCATTTGTGTCTTTTGCCCAAGGGAAGGAAGTAGTGGGGTCTTTGGCCAACTTCTCTAAGGTTACACATGTTCACAGAAATTGTATTCAGTGGGCTCCTCGGATTTACTTTAAGGATGGAATTATTCAGAATTTAGAATCAGAAGTGACAAGGGCTAACAAACTTAAATGCAGCAGCTGTGGTAAAAAGGGAGCTGGTCTTGGCTGCTATATGGAGACATGCCAAAGAAGTTACCATGTGCCTTGTGCATATTACATTCCTGAGTGCAGATGGGATGACACCTATTTAATGTTATGTCCAAAGCATAGAANAATGAAATTTCTAAGCGAAATGAATGCGGATGATGTGAAGCAAGACACTGAGAAAAGAACATCTACACATCTGAATCCCTGCACAACACCGTTTGGATGCCAGACAGAATTTGGTGTTTTGTGGCTCGGATCTTTCTACAGAAGAAAAGTGCTCTGTGGTTGAATT


gi|22244055|gb|BQ858590.1|BQ858590 QGC10K22.yg.ab1 QG_ABCDI lettuce salinas Lactuca sativa cDNA clone QGC10K22, mRNA sequence 

TCGTTTGTTTTTGGCTGCCTT
 ||||| ||||.|||| ||||
CGCAAAGAAAAGCCGAAGGAA

EST sequence (targeted fragment marked with yellow):
AGGAGGAGATTGCAAAAGCAAAACAAGCTTTGGAAAGAAAGAAGAAGCTTGCAGAAAAAGCGGCGTTCAAAGCCAAAAAGAAAGCTGAGAAGGAAGCCGAAAAGAAACGC