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mature miRNA: TCTCCGGGAATCCAATGCGCC
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|57884266|gb|CX649437.1|CX649437 COLD1_35_F10.b1_A029 Root cold Pinus taeda cDNA clone COLD1_35_F10_A029 3', mRNA sequence

TCTCCGGGAATCCAATGCGCC
|||||||||||||||||||||
AGAGGCCCTTAGGTTACGCGG

EST sequence (targeted fragment marked with yellow):
AAATCAAATATCAAATTGATTTATTAGACTTCTGGTAGTATCTCTTCTCATTATACCTATCTATTTCTCTGGATTTAAATCAAGAATTTCTTAACTGAAAATACATATTCTTAACCAGTGAAAAATAAACTCCGTCGGGAACAAAGGTAATTAGTCTGATCCTCAGAAAACCAATTTGATATAAAAGAAGAGATCTCCAAGGAAGATATCATGTGTATTATTAAACAGAAAACAGATCTGCCACAGATCGGCCTCTGGAGAGAGTTTTACCGTAGTTGGCCAACCAAGACTTTGAGATGAATATATTTCCTCTCAGCGTCCAATCTCTGCAGTAGAATATAGCAGATTTCGGTTGACATCCTCCATTTCTCAAGAAAAGGCGGCGGAAACGCATGTGAGTCTGCTCCTCCTGAATCTGGGGCAAAACGGCCACTTGCTTTCTCTGGCTGAAACTAAAATGCAAACCATCGATCCATCGAATCAATAAGAGCACGGAGAACCAGGGCTTCCCCGGAAATCAATACACCTTCCTCCTAAACGCGTTTTCACGCACAGCACGCGCGTTGAAAGGCGTTCAAGAGGAAGGCGCATTGGATTCCCGGAGAGGCTCTCCTTCACGCGTCACTGCGAAGATGCACCTGGAAATGAAGTAGGAGTCTGCCATCCATATTTGGAATACCCAACCTCG



gi|2778329|gb|AA739778.1|AA739778 543 PtIFG2 Pinus taeda cDNA clone 8879M 3', mRNA sequence

TCTCCGGGAATCCAATGCGCC
| ||||||||||||||||||
ANAGGCCCTTAGGTTACGCGN

EST sequence (targeted fragment marked with yellow):
AACTGAAAATACATATTCTTAACCAGTGAAAAATAAACTCCGTCGGGAACAAAGGTAATTAGTCTGATCCTCAGAAAACCAATTTGATATAAAAGAAGAGATCTCCAAGGAAGATATCATGTGTATTATTAAACAGAAAACAGATCTGCCACAGATCGGCCTCTGGAGANAGTTTTACCGTAGTTGGCCAACCAAGACTTTGAGATGAATATATTTCCTCTCAGCGTCCAATCTCTGCAGTAGAATATAGCAGATTTCGGTTGACATCCTCCATTTCTCAAGAAAAGGCGGCGGAAACGCATGTGAGTCTCCTCCTCCTGAATCTGGGGCAAAACGGCCACTTGCTTTCTCTGGCTGAAACTAAAATGCAAACCATCGATCCATCGAATCAATAAGAGCACGGAGAACCAGGGCTTCCCCGGAAATCAATACACCTTCCTCCTAAACGCGTTTTCACGCACAGCACGCGCGTTGAAAGGCGTTCAAGAAGAANGCGCATTGGATTCCCGGANAAGCTCTCCTTCACGCGTCACTGCGAAAATGCCCNGGGAAATGAATTAGGAGTCTGCCCNTCCNTATTTGGAATACCCACCCTCCCAGGGGTNGG