miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: Browse




mature miRNA: TCTTCCCTACTCCTCCCATT
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|6019835|gb|AW064763.1|AW064763 ST35E04 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST35E04, mRNA sequence

TCTTCCCTACTCCTCCCATT
||| ||||||||| ||| .|
AGANGGGATGAGGTGGGAGA

EST sequence (targeted fragment marked with yellow):
GGGTCAGGGAGGGAACATTTCCTAGACGTGGAAACTCTGTAACTAGCTAGTGTGTTTTTAAAATTGGAGGAGAGGGTGGAGTAGGGNAGAGATNNTTTGTATCTTACTTTGCAAGTGTAATGAAGCCCCCGAAATATTATATTTTACAGTACCTTGTAATTTCTANAGGCCAGGTCTTGTCTAAGATATCCGGTATGGACTCNAAGTTTGTNGTTTAAAATTTAGAGCGCTACCTGTAATAGCATAAATATTTTTTGAGGGCAANATATGNTTTCTTTTTTTGGCTTTATTAANGTATTATTTTTAATTTCANGTTTTCTCTAAGAAATCTGGTANGGANGTTGAACGNGTGTTGANAATCTAGAGCACTNGCCTTAGTATTAGTACAACTAATTACGAANGGAGANATCCCTTCTGTCATTTTTATT



gi|74155973|gb|DT624646.1|DT624646 EST1158981 Sequencing ESTs from loblolly pine embryos Pinus taeda cDNA clone PIMAH55 5' end,

TCTTCCCTACTCCTCCCATT
|||||||| ||||||| .|
AGAAGGGAGGAGGAGGAAGA

EST sequence (targeted fragment marked with yellow):
AAATAAGGAAATCCGAGTAGTCATTGTATCAGTCCAGTATGGCCGCCATCCTGACGACATTACCTAGTCCTTCCTCTCTTTCACAGTCGTCCCTAATCAATTCTCTAGCTCTGTGCCCTACTCAAAACCCTCTCTCCAGAAACCTACCTGCTAATCCCCTCGGATTCAGACAAGTTGATCGCAGTTGCACGACATTTAGCTTGTCCGTAAGAAGCAGTTCTAGAATCAGTGGATCACAGTACAGATCTCCCCACAAGGTTCGAGAAGGGCATATTGTGTGTGCACTTGACGATACACAGAAATCTGGGTCGGTTTCTGAATTTCTAAAGGCGGAGGGAGAGCCGGTGCCCGCGGTTAATTCAGAGGCGGCCGGAGGAGGAGGAAATAATTTCGGAGGCAGCAGCGGCGGAGGCGGAGGAGGAGGAGGAGGAGGAAATAATTTCGGAGGCAGCAGCGGCGGAGAAGGAGAAGGAGAAGGAGGAGGAGGGAAGAAAAAAATGGCATTGGCCATGTCCCAGAAATTGACCCTTGGTTATGCTGCTCTGGTTGGAGTGGGCGGACTTATCGGCTATTTAAAAAGTGGAAGCAACAAATCACTTGTATCAGGAGGTTTGTCAGCGCTAGTGCTCTACTATGTTCATACTCAACTTCCATCAAGGCCAACATTTGCATCAGCAGTTGGAATTGGTGTTTCGGCTTTGCTTTTGATAGTAATGGGGTCTCGTTTCAAGAAATCTGGGAAGGTTTTCCCTGCCGGTGTTGTATCCCTTGTCTCTTTGATCATGACTGGTGGTTATCTTCATGGCATATTGCGCAGTTCTCATGCATAGAATAATTATGAAAGTATATGAGGTTGAAAGCCATTTTGTGCGATGAGTGTTTATAAATCACATTGTAATTTAGTTCTTCTAGATTTGGATCTCGGAAATTACTTAGTGATGATATTTTTTTGGTAACAGTGCTCCTAAAAGGATTGATTCCACAGGAATGGGATTGTGGATATATGTTTTGTG



gi|51494721|gb|CV031908.1|CV031908 RTNACL1_4_B09.g1_A029 Roots plus added NaCl Pinus taeda cDNA clone RTNACL1_4_B09_A029 5', mRN

TCTTCCCTACTCCTCCCATT
.|||| |||||| | |||||
GGAAGTGATGAGCATGGTAA

EST sequence (targeted fragment marked with yellow):
GATTCTGAGTCTGGTTCCTCGGATCGTGTTATTGAAATATCTGGGACACCAGAGCAGACACACGCAGCAAAGAGCTTGCTTCAAGCATTTATTGCAACTGGTGGTCAAAGCCAGCCCACACGCAGTGCAGGCCGGGGTTATTAGCACAGGACTAGATTTGATGACTGGCAGCCACGATGTATGATGCCTGGAGCCAGTCTATCATCCCATGCTGAAGCCTCAGCCCAAAACTGAAGATCTGATGAGCAGAGCCGTGGAAAGCCTATTGCCCATGCTTTGGAAGTATTAATTTAGTTGGACTAGCTTCTCAAGGAAATTTTGTTCCCTTGAGTGTTCTTTCCTTTAACTGCCAGTAGTTTTTGGAGTTTATGAACCATGTACTGTAAGGCCCAGATTCAGCATTGAAGTATTGGAAGTTTCAAGACTGAATGATCAAAAGTGGATTTTGTGTTGTCGTATATTATGGCCAATTCCTTCTCCTTAGGATTTTGGAGCCGGATCCCAGACCAAAAATCATTTTGGATTATTGTATCATTTTTTTGAAGAATTTGAGTATACTCACTAAAATGGTACGAGTAGTGAAGGTTTTTGAATAGGATTATTATAAATATGTCATCGCTTTGGTGTATGCCTTCAAAAAAAAAAAAAAAAAAACTGGAGACTAGTTCACAGTATTTTAACTCATTCTGTGTGTAATACCAGTCTCAAAGAAGCCCGAAAAATGGTGGTGTGTCGAGAGTTTAGGGTTC



gi|70785255|gb|DR694903.1|DR694903 EST1084996 Normalized pine embryo library, Lib_D Pinus taeda cDNA clone PWAEI23 3' end, mRNA

TCTTCCCTACTCCTCCCATT
||||| ||||||||| |||
TGAAGGTATGAGGAGGTTAA

EST sequence (targeted fragment marked with yellow):
CTTAAGCAAAACCGCTTTTCCCTGTCGATCACCTTTAACTTTCTAAGCCCTCTTTCTTCGTGAATCTTCTCCATTCTGGTGCGTGTCCTTCACGGGGAACGGCGCAGTGCCGCTATCGCATAATCAAATATATACCACTCAATACAAAATATTTATTCAAAATCCCAAACGAAAGTCCCACTCCTCCCCCCACAAGTCCTGCTCAAAATAGAACAAAACAGAGGTACATTGTTTACCTCCCTCTCAAAATATTTCATGCTGTTTAGCGGATGAATTCTGAACTGCAACATCGACATTCTAGTTTCAAGGTTTCTTCTGGTGATATTCAGTCACCATAGCTGCAGCAGGAGTATTGAACAAAGGCTATAAAGAATCTGTGTAGCAGTGAAATTTTAGATCAGAATGCTTAAAGAAACCTTATTTTGATTTGCTGCTTGAGGTTGGAATCTTTGAAATGGTAGTTCTGTTAATGGTTGGAGAGTTGCTTATGGAGTTTTTACAATAGTAGCTAGGAGCCTTCAGCTTTGAGACAGACATCCTGGAGAAATGTCCAACATGCATGGGGCTACGACATCAGATGTTGAACGTGCAGAGGAAATAAAGGCTATTGCTAATGCAGCATTTCAAGCTCACAAATTTTCCCGAGCCATAGAGCTCTATTCTGAAGCCATTGAGTTGAACAGTCAGAATGCAGTTTATTGGGCAAATCGTGCATTTGCACATACAAAATTGGAGGAGTATGGAAGTGCTATTCACATGCAACAAAAGCTGTTGAAATTGATCCTAAATATACG



gi|21689426|gb|BQ635273.1|BQ635273 NXRV078_C10_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA clone NXRV078_C10 5' simil

TCTTCCCTACTCCTCCCATT
|||||||| ||||||| .|
AGAAGGGAGGAGGAGGAAGA

EST sequence (targeted fragment marked with yellow):
AGCGGCGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGGAGGGAAGAAAAAAATGGCATTGGCCATGTCCCAGAAATTGACCCTTGGTTATGCTGCTCTGGTTGGAGTGGGCGGACTTATCGGCTATTTAAAAAGTGGAAGCAACAAATCACTTGTATCAGGAGGTGTTTCGGCTTTGCTTTTGATAGTAATGGGGTCTCGTTTCAAGAAATCTGGGAAGGTTTTCCCTGCCGGTGTTGTATCCCTTGTCTCTTTGATCATGACTGGTGGTTATCTTCATGGCATATTGCGCAGTTCTCATGCATAGAATAATTATGAAAGTATATGAGGTTGAAAGCCATTTTGTGCGATGAGTGTTTATAAATCACATTGTAATTTANNTCTTCTAGATTTGGATCTCGGAAATTTCTTNNNNATGATATTTCTTTGGTAANNNNNNTCCTAAAANNATCGAT



gi|20664754|gb|BQ290537.1|BQ290537 NXRV045_E03_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA clone NXRV045_E03 5', mRNA

TCTTCCCTACTCCTCCCATT
|||||||.||||||| ||||
AGAAGGGGTGAGGAGAGTAA

EST sequence (targeted fragment marked with yellow):
GACCCANNNNNGTGAGGAGAGGAAGCGGTGAGCATGAGCAGAAAGAAAAGAAACATGGTAGAAGCTTTGTTGAATATCAATTTCCTTCTCCGCTGTCTCTCTGATATCCAGACGGAGGATTAAAGGATCAAGGATCAGCCTGAACAGCCAAGTATCGATTCTCTGGTAGTTTACTCTCCTCGCTCTCTTCGATGTTCGATGCAATGATTTCCTTCTCTTTGTGTTCAGATTCTGTGGATACATCATGTCATTCATGTCTTTACTGCATTTTCATGGACATCGCAAGAGGGTTTGGCCTTGGAGTTATAGCCGTTTTAATTGGAATGAGAGGAGTGGGGAAGAATACCGCTGTCCAAAATCGTAAGAGAAGAGAGAACTCTACTGCTACGCATTGTTCTGCGTTGATAGCTCATCACGCCGATTGGGGAGATTATACAATGCCCAATGGGTGGTCTCTGTTTACCACCCACTGTGTCATTTCCCCTGCCGGATGTTGAAANNNNTGTGTTGCCTTGTGACTTGGAAATCACACGGAAGATCTCNNNNGNNC



gi|34493226|gb|CF475854.1|CF475854 RTWW2_15_G07.g1_A021 Well-watered loblolly pine roots WW2 Pinus taeda cDNA clone RTWW2_15_G07

TCTTCCCTACTCCTCCCATT
.|||||| |||||||||||.
GGAAGGGTTGAGGAGGGTAG

EST sequence (targeted fragment marked with yellow):
CTTACGATGTAACTCTAATGCTCAGCTATGTCCCATACTGTAAAAACAACAATGACTGTTGGGCTGTTTTGCCGTCTATGAACTTGAGGAAACGGAGATGTTTTGTCTGTGGGAGCATTGGGAAATATATTTATGTTGCGGGGGGCCTGACGAACGAGAGGCTCGAGGGCCCTTTTGTGGGTTTGGGAAAAAGGAGCGCCGAGAGATTTAATACCGAGACCAACGAATGGGAAATTTTACCCAGTTTGAACAGAGAGAGGGTCAATGCCATAGGGTTTGTGTTGAATGGGAGGTTTTGTGTGATGGGAGGAGTTGGGAAGGGGTCGAGGACGTCTAGTGAGTTTTATGATCCGGAGGCGAGGAAGTGGGTTTTGGTGCCCAAAATGTGGCCGAAGCCGACTTACTTGTCCTATTTTATGCGGGATGTTGTGGTTGTGGAGAATAGGGCTTATGCAAAAAGGTTAAATTCTGAGCAGACCGTGTTTTCTTTGAGTGTGTGGGAGTATTGCCAGGAGCGTAACCGATGGTCAGATTTGGGTGTTGTGATTCGGCATATTTCGAAGCTGGTTCTATGTGATTACAGGTTGGTGTCTGTTGGTGACGAGCTTTGGATCGTGGGGATGAAACCGGATGCAGAACCAGGGTTCAATGTGTTGGTGTG



gi|68254154|gb|DR386556.1|DR386556 RTHG1_16_A12.b1_A029 Roots plus added mercury Pinus taeda cDNA clone RTHG1_16_A12_A029 3', mR

TCTTCCCTACTCCTCCCATT
. |||||||||||.||| |.
GAAAGGGATGAGGGGGGGAG

EST sequence (targeted fragment marked with yellow):
TTCTTTTGAAGGAAGGTGTATCATCGCATTACCTGTCGTGTCAATGTACCAAATCGTGAAACGTATGCTCTACTAAACAATACAACTTGGGAGAGTGGTGCTCACCAGGGAACCTACTCGGAAAGATCGAAACAAAGCAAGAACGAGAGGGAACCGAGGGAGAGAAAACATTGGTACAAATTAGTGAATAAGAAAAAAAAACATCGAAAACCAAACGTGTCAACGCAGCGGGAGATAAGCGTAGAGCAAGAGAGGGGGGGAGTAGGGAAAGGGAGCGAGCGAGAGAGGATGGTGTATCCGTATAAGCATGTACATAGGAGAACCATGGGAGACGTAGCTGGGGATAGGCGTCAAAAGAGCGGGTCGTCACGTATGCGTGTGGGTTAGCATATAAAGTTATGAACAAGAAAAGGAGCCTGCCGTGGGCATGGCGGATATCTAATACGGGGGAGCGTGGTCATCATCGCGGATGTTGAGCAACGCGATCGAGCTTTTCCAGAAGCAGCTGTTTCGTCTTGGCGTCCAAACCTCTCGCTGAGGTTTCGAGTAGCCGTCTGACTTTGTGCATAAGTCCAGAAGGCCCGTCTACTGGCATCGATCTTGAGTCGAAACGTTGGATATATTCTCTGAACTTGGCATATCCGCTCTCACTGAGCACCTGTTCAAATGACTCCAAAACTTTCACCCTGTACGACTCTTTCATCTCCGCTTCCCTCCTCCACCTGGGGCTAACCCCCATTTCACTAAAATCCATAACGGCCTCCTCCCTCACCCTCGTC



gi|66740593|gb|DR018223.1|DR018223 STRS1_21_G10.b1_A034 Shoot tip pitch canker susceptible Pinus taeda cDNA clone STRS1_21_G10_A

TCTTCCCTACTCCTCCCATT
||||| ||||||||| |||
TGAAGGTATGAGGAGGTTAA

EST sequence (targeted fragment marked with yellow):
GATGCTTAAGAAACCTTATTTTGATTTGCTGCTTGAGGTTGGAATCTTTGAAATGGTAGTTCTGTTAATGGTTGGAGAGTTGCTTATGGAGTTTTTACAATAGTAGCTAGGAGCCTTCAGCTTTGAGACAGACATCCTGGAGAAATGTCCAACATGCATGGGGCTACGACATCAGATGTTGAACGTGCAGAGGAAATAAAGGCTATTGCTAATGCAGCATTTCAAGCTCACAAATTTTCCCGAGCCATAGAGCTCTATTCTGAAGCCATTGAGTTGAACAGTCAGAATGCAGTTTATTGGGCAAATCGTGCATTTGCACATACAAAATTGGAGGAGTATGGAAGTGCTATTCAGGATGCAACAAAAGCTGAAATTGATCCTAAATATACGAAGGGGTACTACCGGAGAGGTGCTGCATATCTAGCCATGGGCAAATTCAAGGAAGCACTAAAAGATTTTCAGCAGGTTAAGAGAATTTGTCCAAATGATCCTGATGCCACCAAGAAGCTTAAAGAAGCCGAGAAAGCAGTCCAAAAACTGAGATTTATTGAGGCCATCTCAGTGAATGATCTTGAGAATCATTCTGTTGTTGAGTCCATTGATTTTCGTTCAATAGAGGTAGAGCCCCAGTATGCTGGTCCTAGAATAGAAGGAGAAAATGTAACATTGGAATTTATAAAGAAAATGATGGATGAGTTCAAGAATCAGAAAAATATACACAGAAGGTATGCATTTCAAATATTATTGCAAGTAAAGGATTTGCTGGAGGCAATGCCATCACTGGTTGACATCAATATTCCAAATGGAAAGCAATTCACTGT



gi|18434417|gb|BM428403.1|BM428403 NXRV_016_C07_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA clone NXRV_016_C07 5' sim

TCTTCCCTACTCCTCCCATT
|||||||| ||||||| .|
AGAAGGGAGGAGGAGGAAGA

EST sequence (targeted fragment marked with yellow):
CAGCGGCGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGGAGGGAAGAAAAAAATGGCATTGGCCATGTCCCAGAAATTGACCCTTGGTTATGCTGCTCTGGTTGGAGTGGGCGGACTTATCGGCTATTTAAAAAGTGGAAGCAACAAATCACTTGTATCAGGAGGTGTTTCGGCTTTGCTTTTGATAGTAATGGGGTCTCGTTTCAAGAAATCTGGGAANNNTTTCCCTGCCGGTGTTGTATCCCTTGTCTCTTTGATCATGACTGGTGGTTATCTTCATGGCATATTGCGCAGTTCTCATGCATAGAATAATTATGAAAGTATATGAGNNTGAAAGCCATTTTGTGCGATGAGTGTTTATAAATCACATTGTAATTTAGTTCTTCTAGATTTGGATCTCGGAAATTTCTTAGTGATGATATTTTTTTGGTAACAGTGCTCCTAAANNNATTGATTCCACAGGAATGGGATTGTGGATAATATGTTTTGTGATGTNNNGCCNNNTAATTGCAGTGTTAAAGATTTTGGTGACTATGNNAAGNNNGGNCTATTACTG



gi|68084108|gb|DR177741.1|DR177741 RTMNUT1_7_D01.b1_A029 Roots minus micronutrients Pinus taeda cDNA clone RTMNUT1_7_D01_A029 3'

TCTTCCCTACTCCTCCCATT
.||||||| |||| |||||
GGAAGGGAGGAGGTTGGTAA

EST sequence (targeted fragment marked with yellow):
CTTCCTGCACAAGACTCCTCTGAACAATCCTCTACCTCGCTTCTTCATTCAGCATGGCTCTATTCAGTACTCTTCTCTGTTTGGTTCTTGCTCTGGGATTAATGATCCCACTCTGCAACCCACAGGCTACCACACCGCCCGCCATTACTGCACCAGAACCATTTCTTGGGCCTATTCCATCATATACCTCACCACCACCTGCTTCTTCCCAGACAACACCGCCAACACCTTATGTCGCGACTGCCCCCACATTTTCTTCTCAAACGCCGCCGCCCCCCGCTACCTCATTCTCCTCGCCCCCGCCTCCATTTGGCACCTCAATCCCCTTGGCTCCAGGCACAGCCACCACTTTGCCAAACGGCGCCGCCTTTACAGCCAATTTAGAGAAGCTTTATATCTCTGGAACCGTCTTGGTTGGAGGAGCTGCCTTGTTTGTTTAAGCTACCTGCGTACAATCTGGTCTCCATCCTCATCTGAAATGGTTGGAGGAGGGAAGGAAGCCTGGAGTCTACGATGATGGGATTCATTGACTCAATCTTACGCGCAGATGCTATAGATGTTGCTCACTTTTATTATTTGAGTTAAATTAGTTGGCAATATTGGTTTTCTGCTTTGTATTATTTTCTTGTAATGCAGTCTGATTCTACTTTACTATATCTACATTCATTGAATGGCCATTATATAGCTTCCTATCATGGACATT



gi|66978011|gb|DR054444.1|DR054444 RTCA1_17_A01.g1_A029 Roots minus calcium Pinus taeda cDNA clone RTCA1_17_A01_A029 5', mRNA se

TCTTCCCTACTCCTCCCATT
|||||||| ||||||| .|
AGAAGGGAGGAGGAGGAAGA

EST sequence (targeted fragment marked with yellow):
GTCCTTCTCTTTCCAGTCGTCCCTAATCAATTCTCTAGCTCTGTGCCCTACTCAAAACCCTCTCTCCAGAAGCCTACCTGCTAATCCCCTCGGATTCAGACAAGTTGATCGCAGTTGCACGACATTTAGCTTGTCGGTAAGAAGCAGTTCTAGAATCAGTGGATCACAGTACAGATCTCCCCACAAGGTTCGAGAAGGGCATATTGTGTGTGCACTTGACGATACACAGAAATCTGGGTCGGTTTCTGAATTTCTAAAGGCGGAGGGAGAGCCGGTGCCCGCGGTTAATTCAGAGGCGGCGGGAGGAGGAGGAAATAATTTCGGAGGCAGCAGCGGCGGAGGCGGAGGAGGAGGAGGAGGAGGAAATAATTTCGGAGGCAGCAGCGGCGGAGAAGGAGAAGGAGAATGAGAAGGAGGAGGAGGGAAGAAAATAATGGCATTGGCCATGTCCCAGACATTGACCCTTGGTTATGCTGCTCTGGTTGGAGTGGGCGGACT



gi|74155843|gb|DT624756.1|DT624756 EST1158851 Sequencing ESTs from loblolly pine embryos Pinus taeda cDNA clone PIMAG12 5' end,

TCTTCCCTACTCCTCCCATT
|||||||| ||||||| .|
AGAAGGGAGGAGGAGGAAGA

EST sequence (targeted fragment marked with yellow):
GACGACATTAGCTAGTCCTTCTCTTTCACAGTCGTCCCTAATCAATTCTCTAGCTCTGTGCCCTACTCAAAACCCTCTCTCCAGAAGCCTACCTGCTAATCCCCTCGGATTCAGACAAGTTGATCGCAGTTGCACGACATTTAGCTTGTCGGTAAGAAGCAGTTCTAGAATCAGTGGATCACAGTACAGATCTCCCCACAAGGTTCGAGAAGGGCATATTGTGTGTGCACTTGACGATACACAGAAATCTGGGTCGGTTTCTGAATTTCTAAAGGCGGAGGGAGAGCCGGTGCCCGCGGTTAATTCAGAGGCGGCGGGAGGAGGAGGAAATAATTTCGGAGGCAGCAGCGGCGGAGGCGGAGGAGGAGGAGGAGGAGGAAATAATTTCGGAGGCAGCAGCGGCGGAGAAGGAGAAGGAGAAGGAGAAGGAGGAGGAGGGAAGAAAAAAATGGCATTGGCCATGTCCCAGAAATTGACCCTTGGTTATGCTGCTCTGGTTGGAGACTGCTGAGCTGCCAACGTGGATACCTTTGCTGTCCACATGAATTTTGCTGATGGCTGCCGTACGGACTTCACGCGTATGGACCTTTTTTTGGCATTGGGCGGACT



gi|67555799|gb|DR096444.1|DR096444 STRR1_28_B07.b1_A033 Stem Response Resistant Pinus taeda cDNA clone STRR1_28_B07_A033 3', mRN

TCTTCCCTACTCCTCCCATT
.||||||| |||||||| .|
GGAAGGGAAGAGGAGGGCGA

EST sequence (targeted fragment marked with yellow):
ATCAAAATTATGATGCTGTTTATATTCTCATATATATATTCCATTTTAATATAATTCAAAACAGGCGNGTTTTTTCCTACTGGAAAAAAAATAAAAATAAAAAATTGATAAACTTCTCATACACTAAATTGCGGGCGGAAGATAAAATCACAAACCAACCCAAACTGAAACATCAATTCAAACCATCCCGACCTTTAGGAAGAGCGGAAATTTAACAGTCCGAGCGAGATGGGCAGGAACTCCCCACGCCTCCTCAAACTGCTGAACGAAGTCGTCTAGCAAACCCTTAGTATACACCACAGCAGACCATGTTCTGAAAAAATTCAGGTACTCCTCCAGTGTCCTCGTCTCTTCGATCTCTAATTCGACGACCGAATCTCCTGCCGCAGCGGGAGGAGAAGGGAAGGGAAAGGGTAGAGTCCTGTACTCTTCGAACACCCATTTCGCGCACGGGCCGAAGAAGGGCAGAAGACGCTCAAAGAACCGGTCAAACACTTCATCCACCGCTGGACTAACGGAGGGCTTCGTGTACGCCCACACCGCAATCACTCCCCCGGGCTTCCTCAGAACGCGCTTGACCTGCGCGTAAAATGTGTTCAGATCGAACCAGTGGATGGCGATGGCCACCGTCACGAGATCCACCGAACCTTCCGGGCCGACGATCGAATGGAGTTCGTCGTCCGTCAGCACGGGCGGCGTGAGGGCGTACCTTATGTTGGGCCTCCTCTCCGCCTGCTGCAGCTGCCGCTCGCTTATGTCTGTGCCTATNACCTGCTCATAATATTTGCTAATCTGAAGGGCAGCTTGGCCATTCCCTGTG



gi|61666304|gb|DN608804.1|DN608804 EST961854 Subtracted pine embryo library, Lib_B Pinus taeda cDNA clone PSAA452, mRNA sequence

TCTTCCCTACTCCTCCCATT
||||||||||||||||||||
AGAAGGGATGAGGAGGGTAA

EST sequence (targeted fragment marked with yellow):
ACCAGGAGAATCCTTTCTGCCAGGGCGTGATAGATCTGCATACCCGCATTGCAAGAAGGAAAATCCAACCCCACAAAAAAAAAATACAGAGCCATCTTTGGCCATATGCTTTCAAAGTCCTGCCTGAAATATTTTATCCCATACAGGGTTTTAATAGATCTATGCTATCAAATATTTAAACTCCAACGAGCTCATATCTCCCTTTCCTCCTTGCCTATCATGGAGGTTCGAAGAATCGTATGACTGTGTCATGCGAGCAGATCTGAAGTGCATCAGCAGCAGAGCTACAAGTGCAGATAAAATAGAGATATGTAGAGAAGGCAATAGGATCCAGGTGAAGTTAAAAAAACCTGTAACAATGCCCAGCTCTCAGATAAGATGCAATCAGGAGCGACTGCATTTGTTTTTTCTCCCCTCCCAGATCCAGTGGCCTTGAAGCTACACGGCATCCCTGTAATACCTGCAGGACATAATCTCCATATTCATCTTCATAGACTATATTGCTGTGTTGCGTTGGAAAGGGAAGTGTGGTGAAGGCAAAAGGAATGGGAGGAGTAGGGAAGACTGGAGCAGAGAATTAATGAAGCAGAGTTTCTCCTTAACCTTAGTCTTACCCTATCCTTCCACACCTCAAGACTTCTAACCATAACACCTCCCTTTCTCGCAACCATCCACCTTCCCTGTTTTCACCCCGCGT



gi|48942126|gb|CO169254.1|CO169254 NDL1_5_F05.g1_A029 Needles control Pinus taeda cDNA clone NDL1_5_F05_A029 5', mRNA sequence

TCTTCCCTACTCCTCCCATT
.|||| |||||| | |||||
GGAAGTGATGAGCATGGTAA

EST sequence (targeted fragment marked with yellow):
CATCAAGTCGTATAACTGATAGCGGGTATTCTGGAGGGTTTCCAAAAAATGTATCCACAACGACTGTCGAGGTTACTATACCAAAAAACGCAGTTGGGTCAGTCCTTGGGAAGAGAGGAAGTAACATTCAAAACATTCGAGAGATATCTGGAGCAAAAGTAAAGTTGCATGATTCTGAGTCTGGTTCCTCGGATCGTGTTATTGAAATATCTGGGACACCAGAACAGACACACGCAGCAAAGAGCTTGCTTCAAGCATTTATTGCAACTGGTGGTCAAAGCCAGCCCACACGCAGTGCAGGCCGGGGTTATTAGCACAGGACTAGATTTGATGACTGGCAGCCACGATGTATGATGCCTGGAGCCAGTCTATCATCCCATGCTGAAGCCTCAGCCCAAAACTGAAGATCTGATGAGCAGAGCCGTGGAAAGCCTATTGCCCATGCTTTGGAAGTATTAATTTAGTTGGACTAGCTTCTCAAGGAAATTTTGTTCCCTTGAGTGTTCTTTCCTTTAACTGCCAGTAGTTTTTGGAGTTTATGAACCATGTACTGTAAGGCCCAGATTCAGCATTGAAGTATTGGAAGTTTCAAGACTGAATGATCAAAAGTGGATTTTGTGTTGTCGTATATTATGGCCAATTCCTTCTCCTTAGGATTTTGGAGCCGGATCCCAGACCAAAAATCATTTTGGATTATTGTATCATTTTTTTGAAGAATTTGAGTATACTCACTAAAATGGTACGAGTAGTGAAGGTTTTTGAATAGGATTATTATAATATGTCATCGCTTTGGTGTATGCCTTCTTTATTCTGTACCCT



gi|34506436|gb|CF477567.1|CF477567 RTWW3_8_E03.g1_A022 Well-watered loblolly pine roots WW3 Pinus taeda cDNA clone RTWW3_8_E03_A

TCTTCCCTACTCCTCCCATT
||||| ||||||||| |||
TGAAGGTATGAGGAGGTTAA

EST sequence (targeted fragment marked with yellow):
AATAGAACTAAGAGAGGTACATTGTTTACCTCCCTCTCAAATATTTCATGCTGTTTAGCGGATGAATTCTGAACTGCAACATCGACATTCTAGTTTCAAGGTTTCTTCTGGTGATATTCAGTCACCATAACTGCAGCAGGAGTATTGAACAAAGGCTATAAAGAATCTGTGTAGCAGTGAAATTTTAGATCAGGATGCTTAAAGAAACCTTATTTTGATTTGCTGCTTGAGGTTGGAATCTTTGAAATGGTAGTTCTGTTAATGGTTGGAGAGTTGCTTATGGAGTTTTTACAATAGTAGCTAGGAGCCTTCAGCTTTGAGACAGACATCCTGGAGAAATGTCCAACATGCATGGGGCTACGACATCAGATGTTGAACGTGCAGAGGAAATAAAGGCTATTGCTAATGCAGCATTTCAAGCTCACAAATTTTCCCGAGCCATAGAGCTCTATTCTGAAGCCATTGAGTTGAACAGTCAGAATGCAGTTTATTGGGCAAATCGTGCATTTGCACATACAAAATTGGAGGAGTATGGAAGTGCTATTCAGGATGCAACAAAAGCTGTTGAAATTGATCCTAAATATACGAAGGGGTACTACCGGAGAGGTGCTGCATATCTAGCCATGGGCAAATTCAAGGAAGCACTAAAAGATTTTCAGCAGGTTAAGAGAATTTGTCCAAATGATCCTGATGCCAC