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mature miRNA: TCTTCCCTACTCCTCCCATTCC
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|257196078|gb|GT052631.1|GT052631 GQ04109.B7P2_K22 GQ041 - Shoot tip - Dormant (Normalized) Picea glauca cDNA clone GQ04109_K2 

TCTTCCCTACTCCTCCCATTCC
||||||||||||||||||||||
AGAAGGGATGAGGAGGGTAAGG

EST sequence (targeted fragment marked with yellow):
AAAGGAATATAAGCTGAACATATAAATATCTAAGGGCAAACCTGTGCCATGCGAGCAGATCTGAACAGCAACAGAATTACAAGTGCAGATAAAATAGGGATGTGTAGTGAAGGCAATAGGTCTAGGTGGAGTTGGAAAAACTGTAACAATGCCTATATCACATCAGATCAGATGAGATCACGAGTGACTGCATGAGTTTTTCCTCTCCTCCCAGACCCAGTGGCCTTAAAACTACATGACATCCCTGTAATACCTACAGGACATAATCTCCATATTCTTCATAGACTTTCTACTGTGTTGCGTTGGGAAGGGAACTGTGATGAAGGCAACAGGAATGGGAGGAGTAGGGAAGACTGGTGAGATTAGAGCAAAGAATTATTGAAGCAGACTCACTTAACCGCAGTCTTACCCTATCCTTCCACTCCTCCAAACTTCATCCATACACATCCCTTTCTCACAACCAACGGCCTTCCCTTCATTCA


gi|225911749|gb|GO360075.1|GO360075 GQ02819.B7.r_H20 GQ028 - Cambium / phloem scrappings (Normalized library ) Picea glauca cDNA 

TCTTCCCTACTCCTCCCATTCC
.|||||| |||||||||||. |
GGAAGGGTTGAGGAGGGTAGTG

EST sequence (targeted fragment marked with yellow):
ATGTAACCCTGATGCTCAGCTATGTTCCACACTGTGAAAAACGCGATGACTGTTGGGCTGTTTTGCCGTCTATGAACTTGAGGAAGCGGAGATGTTTTGTCTGTGGAAGCATTGGGAAATACATTTATGTTGCCGGGGGCCTGACGAACGAGAGGCTGGAGGGCCCTTTTTTGGGTTTGGGAAAAAAGAGCGCCGAGAGATTCGATACCGAGATCAACAAATGGGAAATTTTACCCGATTTGAACAGAGAGAGGGTTAATGCCATAGGGTTTGTGTTGAATGGGAAGTTTTGTGTGATGGGAGGAGTTGGGAAGGGGTCGAGGACGTCTAGTGAGTTTTATGATCCGGAGGCGAGGGAGTGGGTTTTGGTGCCCAAAATGTGGCCGAAGCCGACTTACTTGTCCTATTTTATGCGGGATGTTGTGGTGGTGGAGAATAGGGCTTATGCAAAGAGGTTAAATTCTGAGCAGACCGTGTTTTCGCTGAGTGTGTGGGAGTATTGCCAGGAGCGTAACCGATGGTTAGATTTGGGTGTTGTGGTTCGGCATATTTCGAAGCTCGTTCTGTGTGATTATAGGTTGGTGTGTGTTGGCGACGAGCTTTGGGTTGTGGGAATGAAGCCGGAAGCAGAACCAGGTTTCAATGTGTTGGTGTGCAGGCCCGGCACGCGGTTGGAGAGGGAAGAGGGGCTTAGATGGAGGAGGTTGGAATTGGGATGGGACAAGTACTTTGTTCTGTTGCTCTCTTGCTTAGCTTTCTAGTAGGAAAGATGAAATTTCTGTTTCAGGTGCCTGCCTGATCTATTTAAATGCTATTTGGGTATATCTAATTCGG


gi|225917797|gb|GO369849.1|GO369849 GQ04004.B7P1_M09 GQ040 - Shoot tip - Active growth (Normalized) Picea glauca cDNA clone GQ04 

TCTTCCCTACTCCTCCCATTCC
||||||| ||||| |||| .||
AGAAGGGTTGAGGCGGGTTGGG

EST sequence (targeted fragment marked with yellow):
TTGAGATAAGTTTTGTCGCGGCAGCACCAGAGTGGAAAACCTCAGGTCGTGGGGATCGTGGGGTTGGGCGGAGTTGGGAAGACAACGGTAGCAAACAAATTCTTCCATAAAGAGAGTCCATCTTACCTTAACTCCTGCTTTTTCACTGTGAATCAAAATGCAGGTAGCAGCTCTTTACATTCTTTGTTCGGAGAAGTTTTAAAGCGATTGACTCGCTCGGATGTGCAAATAAACAGCGTTGGTGAAGTGAAAAAATTGTTTGAAGAGTATGAACCCTCGTTAGAAATGATTCAAAAGTTTTCCTCATTAGTAATTTTAGATGGAATAGATGATTTTGATCAGTGGAATGCACTCTTGCCAATCCAAGACGTTCTTCATTCCGATAGTTTAATATTGATTACATCTCGGAACAGAAATGTTCTTACAGGCTCAGGAGTTGAAGCAGCATCGATTTATCAGTTAAATGGCCTTAATACACAGTATTCCCGAAAGCTTTTTTGCTTCCATGCCTTCCACACAGCATCTCCGCTGCAAGGATATCAATGCTTAGTGGATGAATTTTTAACTGTCTGCGAGGGATTGCCGTTATTATTGAAAGTTATTGGGGCATCTTTTTATAAAAAAGACATATCTGATTGGGAAGGTCAATTGTACAGGTTTCAACCAATACTTCCCCATGAGACACAAAAAAAACTTAAATTTAGTTACGATGCACTCAATATAGAGGAAAAAAATATATTCTTGGATATTGCCAGTTCTTTCATTGGAGAAACGACTGATGCTGCTATAATACAATGGCGAGGACCAGGCTGGGGAGGTTTGCCCGGGTTTCAAAGTCTACTGGAAAAACGTCTAGTGGAGGTGGATAGTAAAATTGTATAAATATGCATGACCATCT