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mature miRNA: TCTTCCCTACTCCTCCCATTCC
species: Pinus banksiana
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|292547781|gb|GW762852.1|GW762852 PBA025.C21_E01 Pba02-Xylem-wounded-Norm (PBA02) Pinus banksiana cDNA clone PBA025_E01 5', mR 

TCTTCCCTACTCCTCCCATTCC
||||||| ||||| ||||||||
AGAAGGGCTGAGGTGGGTAAGG

EST sequence (targeted fragment marked with yellow):
GGGGGAAAAACTGCATGTTTCCATGGAGTTTTGGAGGAGTGTCTCTGTCGATCAAGGTTAATTGCAATGGATTTTTACTGTTATTATCCGTCAATGCCTTGTCAAATGCTTTAAACTGCATGTTTCCATGGAGTTTTGGAGAAGGGTCTCTGTCGATCAAGACAGTAGGGGTAAAGATACTGGAAAATGGCCCAGCGGAAGAAGAAGAAGAGGTGGTGGTAGAGAGTTACCTGGAATGGGTGGAGTCGGGAAGAATAGGGTTGAATGCATTATCAATTATACATGCTTGTGGTGTTTGATGAACAACAAAGTGGGGTGTTAGTCACATGGGTGGTGATGTCATGTAGTGCGTCACAAGATATTGCACTATGGTTGGACCACCTCTTGCATAGGTGTTATCAACTTCGTCCTGATTGGAAGGTCAATGCCTTTATGGTTGATGATGCATTGGCTGAAATCATTGCTTTGAGGTTGTATTCCCCCATCAATGGATTCAGAGCCTTTTTTGGATCATGATACCAGATGCAATACATTCAATCTCCCTCCTGAGATAATTATAGAGGCATCAAATCTTGATACAGATGCTAGCACAACTATTATGGTTGATGACAATGTTGATGCAAATGTTGAAGAATTTTAGTTGGCTATAGAAAGAAGTTGTTTGGCTACTTAGACGTGTTGAGGAATAGTCCTCTTGTTACTTTGAGCAAGACAAAACAAATGATTGGCTTGGTTAAAAAATGATGGATGATACTAATGATCTTCATATTATGGATTATGAC


gi|292541638|gb|GW757871.1|GW757871 PBA0211.CR_G18 Pba02-Xylem-wounded-Norm (PBA02) Pinus banksiana cDNA clone PBA0211_G18 3', m 

TCTTCCCTACTCCTCCCATTCC
|||.||||||||| ||| .|||
AGAGGGGATGAGGTGGGAGAGG

EST sequence (targeted fragment marked with yellow):
GGAGGGCAGATGTCCGTGAAGCAGCACTTCAAAGCCTTCTTGAACTTACTCGTGCTGGCAAGGCAGAGGCTGCTGGTTTGACTGACGAGGAAGGGAAATTAAGGCAAGTTCTTCAGGATCGCATAACTGATATCAGCCAGATGTCACCAGATGATTTAGGGGCAGCTAGGGAGGAAAGGCAATTGGTTGATTCTCTTTGGCAAGCATGTTATAACGAGCCTTCCGCTCTTCGAGCGAATGGCCTGCTTCTTCTCCCAGGAGAGGATGAGCTGCCTCCAGACATTGCAAGTAAACACTTTGAGCCCCCACTTAGAGCATGGGCAGCACCTCGATCTGGCACGACAGGTTCCAGTACAACTGATAAGAAAGAGAGTCCTGTTTTTCTGCTTGGTGCAGGTCCAGTCACCCCTAGTGGGAATTCTGAAGATAGTAGAAATGGAGAATCGGCAGGTAACTCTAGTGGGGGTCAGGGAGGGAACATTTCCTAGACGTGGAAACTCTGTAACTAGCTAGTGTGTTTTTAAAATTGGAGGAGAGGGTGGAGTAGGGGAGAGATAATTTGTATCTTACTTTGCAAGTGTAATGAAGCCCCCGAAATATTATATTTTACAGTACCTTGTACTTTCTAGAGGCCAGGTCTTGTCTAAGATATCCGATATGGAGTCGAAGTTGTGTTTAAAATTTAGAGCACTACCTGTAATAGCATAAATAGTTTTTGAGACAAAAAAAAAAAAAAAA