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mature miRNA: TCTTCCCTATTCCTCCCATT
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|225913549|gb|GO367406.1|GO367406 GQ03715.SP6.r_G24 GQ037 - Shoot tip - Dormant Picea glauca cDNA clone GQ03715_G24 3', mRNA s 

TCTTCCCTATTCCTCCCATT
|||||||.|||| | || ||
AGAAGGGGTAAGAAAGGCAA

EST sequence (targeted fragment marked with yellow):
TTGTGCATTGCTGAACTCCACGGGCGGCTTGGATAAAATTAGCGTGCTGGCTTCGCCCCCATATATCTGTAATTCAAACCAACTGGGAGAAGTTTCTCTTGTTATCCGCCCACCTTCCATTGCTCCTGAACCTAATCAAGATAATTCAGAAAACCCAACGGAAAGAATGGGGAAGAAAGGATCAAATGCATGGAAGATTGCAGTTGGTGCAGCACTAGGTGGGATTGCAGCCCTTTTGGCCTTGGTTCTATTGATTTTTTGGGGAAGAAAGGAAGTGGACAAGGCTAAGATCGCTAAAATGGAGAAGCATGCAGACAATGAGGAGACATTGCAAACTTCATTGATAGGGAATAGCAGGGCTCCCACTGCAGGAGGCACAAGGACAAAGCCTGTTCTAGAGAATGAAGATTCAACATAGAATATGCACAGGAGTTGAAGTTCTCTGAGGCCGAAGTTTGGTTTCCTAGGCTTGTTTTGTCATTGGTGTTGGCAGCAATATACACGCACATCGAGCCATTTCCCGAACAAGTTGAAGGCATCTGGGAAGTTCTCAGATGGCAGCTGTAGACACCGTGAAGAGATTCTTTCTGCTGTGAAACTCGGATTTGTGGATTGATGAGATTTTATTCTTTTCCATGCTTAATTTTTCAAGCTCCTATTCATTGTGGTCTTCCATAAGGTGTGACTGACTAAATCAGATTCTTTTTTGTAATCTTAAACTTTTGTTATACCTAATGAATTAGTTGAATGCAACGTGCAGACTTTTTTTCATAAAAAA


gi|225915936|gb|GO366237.1|GO366237 GQ03613.SP6.r3_M24 GQ036 - Shoot tip - Active growth Picea glauca cDNA clone GQ03613_M24 3', 

TCTTCCCTATTCCTCCCATT
|||||| ||||| .| ||||
AGAAGGAATAAGAGGAGTAA

EST sequence (targeted fragment marked with yellow):
AATATCATTGGCCCCAAAAGCACTAGATCTGGCCTGATTTGTAGTGGGGATAGCTTGTCAAGGTTCAGTTGGTGAAAATGGCGGTCGGTAAGCATGGTCGTCAGGATAAACAGGCATCATCGTCACTGTGTTTTAGAGGGACAGTGGTTATTTTTGTTGGGGTTTGTATTCTTGCTGTATGGATGATGAACTCATCTTCACCTGTGCCAGCAGAAAGACAAAGCTTCAATGGCATTAGGAAAATCAGTCCAGAGAGCCGGGGGAAACCGAGTGATTCTTCTCCCGGGTCTCAAGAGACAAGGGAGAAGACGAAAGATTCTTCTGCGGTGTCTTTTGAAGACAGCCCTGGAGATCTGCCAGAGGATGCCATCAAAGGGGATGATGGGGAGGAGCTTGAGAAGATAAAGAACCCTGAGATCCAGCAGAAGACCCAACAAGATAAAACATCTGATGATGGGGCTGCTAATTCAAATGAGGAGAATAAGGAAGATGAGAACTCTGATGGTGCTGGGAAATCGATCGAAGAAAATATATCAGATGGGGATGGGAATTCAAATGAAGAAGAAAAATCAGATGGGGATGTGAAACCAAAAGAGGAGAAGTCGGATGAAAATCAAAATGAAGGAAAAGATGAGGAGGATGTGACCGTGAATGACAATGAAGAAACTACTGAGAAAGGAGGTAATGAAACTGACAGTCAGGAAAAGAAAGACATAAAAAA


gi|255779374|gb|GR953561.1|GR953561 GQ02802.B7P1_F19 GQ028 - Cambium / phloem scrappings (Normalized library ) Picea glauca cDNA 

TCTTCCCTATTCCTCCCATT
||||| |||||||| ||||
AGAAGAGATAAGGAAGGTAT

EST sequence (targeted fragment marked with yellow):
GGAAGGTACGGTATATTGGTGTTTCTAATGAAACATCTTATGGAGTTATGGAGTTTGTTAATGCTGCAAAGCTGGAAGGTTTACCAAAGATCATCAGTATACAAAACAGTTACAGTTTGCTAGTACGGTGTCATTTTGAAGTTGATCTGATGGAGGTGTGCATGCCTCAAAATTCTAATGTTGGTCTATTGGCATATTCTCCATTGGCTGGTGGGGCTTTGACTGGGAAGTATACAAATCCTGATTATATAGCAGCAAAGAAAGGGAGGTTTAATCTCTTTCCAGGATACATGAGTCGCTATAATAGTTCTTTGGCTAAGGAAGCTACTGAAAAGTATATTCAATTGGCATATCAACATGACCTTACCCCTGTTCAAATTGCACTTGGTTTCATACGTGATCGCCCATTTGTGACAAGCACAATTATTGGTGCAACATCCATGGAGCAGCTTAAGGAAAATATTTCTGCTTTCACTGCAACCAGGCCCTTATATCCAGAAGTTATGGAAGGAATAGAGAAGATATTTTCCAGATACAGAGATCCAGCAATGACATAGTTTTTCACTCAAAGGATTGTTTTTTAATGTCCAGTTTGAAAACCCAGGCAATGACACAAAACACATTCCAGATAAAACATTCTTCTTATTCAACATGGGATCCGACTTTCCAATGTGTGTATTTCCTGTTGAATTTAGGTTGGACCTTGGTTGTGCAAATTTATGTGCTTGATTTGATCAGAAAATGCCATATCTCTTGGTAAGACCACATCCATATTTCAAAATATTTTATTTACCATTTGTAAAGAAACTGTGGGAGGGCATATACATCA