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mature miRNA: TCTTCTCTATTCCTCCCATTC
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211892522|gb|CO484844.2|CO484844 GQ0208.TB_O16 GQ020: Clean ROOTS systems -Diurnal cycle Picea glauca cDNA clone GQ0208_O16 3

TCTTCTCTATTCCTCCCATTC
|||||||| || |||| ||||
AGAAGAGAGAAAGAGGCTAAG

EST sequence (targeted fragment marked with yellow):
GCTTTGCAGAATCGGAGAAAGAGAGAAGACAAGCGGCTGTCTTCAACAAAGCGACTTGCAGTTCTCACAGGAGAATCGTAGCCATGTCTGGGAGGAAGAAGGTTCGAGAGCCCAAAGAGGAGACTGTTACACTAGGTCCTACTGTTCGAGAGAATGAGCAGGTGTTTGGAGTGGCGCACATATTTGCATCCTTCAATGACACCTTTGTGCATGTGACTGATCTCTCTGGGAAGGAAACAATGGCACGGGTAACTGGGGGTATGAAGGTTAAGGCTGATAGAGACGAATCTTCCCCCTATGCTGCTATGCTAGCAGCACAGGATGTTGCACAGAGATGCAAGGAGCTTGGTATAACTGCCTTGCATATTAAGCTTCGTGCAACAGGAGGGAATAAAACAAAGACGCCTGGGCCTGGAGCACAGTCTGCTCTTCGTGCTCTTGCACGTTCTGGCTTGAAGATTGGTCGTATAGAGGATGTGACTCCAATCCCCACTGACAGCACTAGAAGAAAGGGTGGACGAAGAGGAAGAAGGCTGTAGGGTGCACTTTGGGTGCTCTATCTCGAAAGTGCTGATTTTGATTTATGGTATAAGAAAGGGTGGACGAAGAGGAAGAAGGTTGTAGGGTGCACTTTAAGGTGCTGATTTTGTTTCATGGTATTATTATTTTAGTTATTGCAGGACTTTTGCTAAGAAATACAATGTTTAGAGGGGTTGTCATGGAATATTAAAATTGTTTTTTTAACCAAAAAATCCAACTTTTAAAAAACAATGAGGTAGGCAAACTAAGCAAATTACTATTTTGGAGGCAGAGTGGGCTTTATATATT



gi|255779374|gb|GR953561.1|GR953561 GQ02802.B7P1_F19 GQ028 - Cambium / phloem scrappings (Normalized library ) Picea glauca cDNA

TCTTCTCTATTCCTCCCATTC
|||||||||||||| ||||
AGAAGAGATAAGGAAGGTATT

EST sequence (targeted fragment marked with yellow):
GGAAGGTACGGTATATTGGTGTTTCTAATGAAACATCTTATGGAGTTATGGAGTTTGTTAATGCTGCAAAGCTGGAAGGTTTACCAAAGATCATCAGTATACAAAACAGTTACAGTTTGCTAGTACGGTGTCATTTTGAAGTTGATCTGATGGAGGTGTGCATGCCTCAAAATTCTAATGTTGGTCTATTGGCATATTCTCCATTGGCTGGTGGGGCTTTGACTGGGAAGTATACAAATCCTGATTATATAGCAGCAAAGAAAGGGAGGTTTAATCTCTTTCCAGGATACATGAGTCGCTATAATAGTTCTTTGGCTAAGGAAGCTACTGAAAAGTATATTCAATTGGCATATCAACATGACCTTACCCCTGTTCAAATTGCACTTGGTTTCATACGTGATCGCCCATTTGTGACAAGCACAATTATTGGTGCAACATCCATGGAGCAGCTTAAGGAAAATATTTCTGCTTTCACTGCAACCAGGCCCTTATATCCAGAAGTTATGGAAGGAATAGAGAAGATATTTTCCAGATACAGAGATCCAGCAATGACATAGTTTTTCACTCAAAGGATTGTTTTTTAATGTCCAGTTTGAAAACCCAGGCAATGACACAAAACACATTCCAGATAAAACATTCTTCTTATTCAACATGGGATCCGACTTTCCAATGTGTGTATTTCCTGTTGAATTTAGGTTGGACCTTGGTTGTGCAAATTTATGTGCTTGATTTGATCAGAAAATGCCATATCTCTTGGTAAGACCACATCCATATTTCAAAATATTTTATTTACCATTTGTAAAGAAACTGTGGGAGGGCATATACATCA