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mature miRNA: TGAACTGGGTTTGTTGGCTGC
species: Gossypium raimondii
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|48818992|gb|CO120305.1|CO120305 GR__Eb023L15.r GR__Eb Gossypium raimondii cDNA clone GR__Eb023L15 3', mRNA sequence

TGAACTGGGTTTGTTGGCTGC
|||||||||||||||||||||
ACTTGACCCAAACAACCGACG

EST sequence (targeted fragment marked with yellow):
ACACATACTACCTCCTTAAATATATATATATATCAGTTGAACAGAGTCAGGAAGGAGCTTGTAAATCTCAGCCCAGTCCTGGTTAGAACTGATGTTGTTGGCTCCTGAAAAGTGTTTTTATTAACTAGATTTGATATCTGCTTCAAAAAAGCAGATCAGCAGCCAACAAACCCAGTTCAAATCAAGATACTGGAATACTGAACTGCATCATGAATTGCAGGTCTTGGGGGTGATTCATCGAGTCAACCACCATCCACCATCTACATCCTTCCCCTATATAAACTCTGTAATATATATATATATATATACACATAGAAACAAATTAAAACAGGTTATAAAAGAGATTGTGGATCTTGGTTCTTCGCTTCTTATACTATCAATTATCTACATCTTCCCCCATATAAACTCTGCAAAAATATATTAAAACAATTTGTAAAAGACAGTGGTTTGTTCATTGCTTCTCTTAACATCTACAATCTACTTCCTTCCCCTATATCCTAAAAGTTACTGAGAATAATTAGTTCATCCTTCCCAATAAAATTCTCACATTTTATGATAAAAAATAATAATAAAAGAGACGACTTAAAACAAATTACGGAAGGCAGGAAAGTTAGATATCAAATACAGTTGGCATGGTGGTTTCACAGCCGCTATTTCACAATAGCTAAAGATCTCACAGTAGAATCCAAAGCAGAAAAATCAAATAGAGAGGCTGATACTTTAAGGGTTTCGAAATTTACCTTATTTGAACGGAGTCGCGGTGAGATGCTCGCTTGGCGGTCAG



gi|48794273|gb|CO095587.1|CO095587 GR__Ea18E17.f GR__Ea Gossypium raimondii cDNA clone GR__Ea18E17 5', mRNA sequence

TGAACTGGGTTTGTTGGCTGC
|||||||..|||..||.|||
ACTTGACTTAAATGACTGACA

EST sequence (targeted fragment marked with yellow):
CCAGTTAACTTGACTAAAAATTATTATAAATGAAACTGAAAATACTAATTCAAGTCCTAACATATTGCATATACACAGTCAGTAAATTCAGTTCAAACTCAAAAGTTAATAGTAACAATACTATGGAACTAAACCCCATTGGCACTGAAAATCCTTGAAAATGGAAAAAATAATTACCTAGCTAGCATTACTGGCATCATCTTCTTCTTCTTCTTCTCCTTCACCCTCATTGTCCAAGGCTTCCTTTGCAGCTTCCTTGTACTACATTAATTCTCACAAACTGCACCATCAAAATCAGGTTTTGGGGAACTTATGCAGCCCCAAGCTCAGTCTCAGGCAAATCAATATCATTGTTGATCAAAGGACTAGGGGGAAAAGGGGGACAAGTAGGGGGTAACATTCTTGAGTAAAGCTGATCCAATTGGGGAAAATAAGGGCAGGTTTTAAAATGCCGACAGCGCTTCTTGCCATTTTCTTTGCTCTTCCTAAAATATTTGTTAAGGTTTTCCCATTTTTCTTTGCATCTTTTAGCACTTCTTTGATAACCAAGGGAAGCCATGAAAGAGCTTACCTCTTCCCAAACAGGCCCTTTCAATCCAGGTTCTCTAAACTTTGCTTCTAAGTCGCATCTTACTTGGATCAATGCCTCAACTTCAGCTCTAGGCCAC



gi|48811751|gb|CO113064.1|CO113064 GR__Eb013B08.f GR__Eb Gossypium raimondii cDNA clone GR__Eb013B08 5', mRNA sequence

TGAACTGGGTTTGTTGGCTGC
|||| ||||||.| ||||||
ACTTAACCCAAGCCTCCGACG

EST sequence (targeted fragment marked with yellow):
CGAAAAAAAAAAAATGTTCCTATCGGTCCCCCCCATGGAAACATAACAAAAATGCTACTGGTCACCTTATTTAAACATGTATTAAAAAAATTGTCTCTCGTAATGGTAAAAAAAAAAATTTTAAAAAAAAAGTCAAAATTTAAGCATGTTATAGTTGAAATTTTTCTTTCGAGGAGCTCTTGTAACTCTTCAAACGAAAATGATCCCCCCCTATAACTTAAAACTCTTTAAATGTTTAACCCCAAAAAGGGATTGATAAAAAAATTACAATACCTAACGCCGACCTCCGGTTTTCTTAGCCGCAACTGGGGGAAATAAATCCTCATCATCCGACTCCACATCTCCATACTGCCACATTCCACGCCGACCAGTTTTCGCTTCCTCCTGACACTCTTCCAATTTATTAAGCATCAATTTTCTCTCCTTCGATTCCCATTTCTTCCTCTTCTCCAACCTAACAAAACCGGCCTTCAGCATTGCTGCATTGATGCTATCCCCCGGGGCCTCGGGAAAGAGGCTTACAACGAGACAAGTACCAGTTCCCTGCCCCTTGACTTTTCCCCCAAAGGGATCCCTTTCCTCTATCACTGCCTTGAATTGCAGGGAACTTCCTAATGGCTGCTCACTCAAAAACTGGGCAGCCTCCGAACCCAATTCATCATC