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mature miRNA: TGAAGAAGACTTGGAGCTTCGA
species: Picea glauca
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|211864614|gb|DV970733.2|DV970733 GQ0046.TB_N09 GQ004: Non-lignified secondary xylem from mature trees Picea glauca cDNA clone

TGAAGAAGACTTGGAGCTTCGA
| |||||||||||.|||| | |
AGTTCTTCTGAACTTCGACGAT

EST sequence (targeted fragment marked with yellow):
CAGTATGTGGGTTCATGTTAGTAATGATTTTAATGGCGAATTATGTCCATGGCAACGAGTTCTGGCCTGCTCAGGATCAGAACCATAATCAGCAGCGTAGTCCCGTTAGTCGGGTGAGATTCAGTCATGGTCAGTATATAGATAATCATCTTGAGCATTTGGATCATTCTACAATGATTTCTTACAACAACAAGATAACTATCAGAAGGCTCTTGGTGGAGAGAATCATATAAGTAAAATTGCAGATCCAGTTTATGTATGGTTTGATCTGGTTGAAAATATATTTTTACTATCAATGTGAAGGCTCTTGGTGGAGAGAATCATATAAGTAAAATTGCAGATCCAGTCTATGTATGGTTTGATCTGGTTGAAAATATATTTTTACTATCAATGTGCAGTACAGTTAGTTTTCAGAGATAGTTTGTAATTTGTGTAGCAGCTTCAAGTCCTCTTGAAGTGTTCTCTGCTATCAAAATTCTATCTTTGAATGTATCAATTTGCTTTTGCTTTTGGGTTGAATTGAACGTTTTAACTTCATATGGATGGCCAGCATTGAGTTCTAGACTCTGATTTTTCTTTTGAGTTGGATTGAACGTTTAGACTTAATATGGGTTGGTCAGCATTGGGTGTACAGTTAGTTTTCAGAGATAATCAATGTGTTGTACAGTTAGTTTTCAGAGATAGTTTGTAATTTGTGTAGCAGCTTCAAGTCTTCTTGAAGTGGTCTCTGCTATCAAAATTC



gi|270051893|gb|GT739963.1|GT739963 GQ0186.B7P4_F11 GQ018: Clean ROOTS systems - N treatments Picea glauca cDNA clone GQ0186_F11

TGAAGAAGACTTGGAGCTTCGA
||||||| |||||||.|| ||
ACTTCTTATGAACCTTGACGCC

EST sequence (targeted fragment marked with yellow):
ATCTCATCGAATGCTTCCGTTGAGCTACCGATCTGAGTGTTGCAGGATTTGGCTGTGAGGTCGACGAGAATGGAGATGGAGAAAATGTTTCATATGAACGCCGGCCTCGGAGACTCCAGCTATGCTCAGAATTCCACTATCTTACAGAAGAGGGCATTAGACCTGGTAGAGCCTATGTTGGAGGAAAGCATTTTATCCATGAAAATGATGGAATGCAAAACATTTTGCATCGCAGAGCTGGGTTGTTCGTCTGGACCTAATGCTCTCTTGGGTACTGAAAATATTATAAAGGTTCTCGAAGCCAAATATTTGTCAGCGGGCAATCCTGTTCCGCAGTTCCAAGTATTCTTCAATGATCTTCCTTCGACTGATTTCAATTCGCTCTTTAGGACCCTGCCTCCCTCAGTAGTGACTGATGGGAATTACCCGGCTAGATCATATTTCTCAGCAGGAGTACCGGGGTCATTCTACGGAAGGCTATTTCCAGACAAGAGTCTTCATTTCGTTCATTCTTCGTACAGTCTTCACTGGCTTTCTCAGGTTCCCTCTGAGGTTTTAGAAAAGAATTCAGTGACATGGAACAAGGGCAAAATCTCTGCCGGAGGATCTCCCACTGTTGGACAAGCTTATTTTCGTCAATATCAGAAGGATTTCAACGCGTTTCTGAAAGCCCGGGCAGAGGAGATGGTTCGGGGAGGCCGCATGTTTTTGCTGTTAACGGGTCGAACATCTCGCGATCCAAATGATCAGGGTTTCATTGCC



gi|211902706|gb|CK435907.2|CK435907 GQ0041.TB_P03 GQ004: Non-lignified secondary xylem from mature trees Picea glauca cDNA clone

TGAAGAAGACTTGGAGCTTCGA
| |||||||||||.|||| | |
AGTTCTTCTGAACTTCGACGAT

EST sequence (targeted fragment marked with yellow):
ATTCAGTCATGGTCAGTATAGAGATAATCATCTTGAGCATTTGGATCATTCTACAATGATTTCTTACAACAACAAGATAACTATCAGAAGGCTCTTGGTGGAGAGAATCATATAAGTAAAATTGCAGATCCAGTTTATGTATGGTTTGATCTGGTTGAAAATATATTTTTACTATCAATGTGAAGGCTCTTGGTGGAGAGAATCATATAAGTAAAATTGCAGATCCAGTCTATGTATGGTTTGATCTGGTTGAAAATATATTTTTACTATCAATGTGCTGTACAGTTAGTTTTCAGAGATAGTTTATAATTTGTGTAGCAGCTTCAAGTCCTCTTGAAGTGGTCTCTGCTATCAAAATTCTATCTTTGAATGTATCAATTTGCTTTTGCTGTTGGGTTGAATTGAACGTTTTAACTTCATATGGATGGCCAGCATTGGGTTCTAGACTCTGATTTTTCTTTTGAGTTGGATTGAACGTTTAGACTTAATATGGATTGGTCAGCATTGGGTGTACAGTTAGTTTTCAGAGATAATCAATGTGTTGTACAGTTAGTTTTCAGAGATAGTTTGTAATTTGTGTAGCAGCTTCAAGTCTTCTTGAAGTGGTCTCTGCTATCAAAATTCTATCTTTGAATGTATCAATTTGCTTTTCTT