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mature miRNA: TGAAGCTGCCAGCATGATCTA
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|57807880|gb|CX609160.1|CX609160 ANR1_11_B10.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone ANR1_11_B10_A002 3', mRNA sequ

TGAAGCTGCCAGCATGATCTA
| |||||||||||||.|||||
AGTTCGACGGTCGTATTAGAT

EST sequence (targeted fragment marked with yellow):
ACAATCACTTATATCTTTTTTGAAATAGATTATGCTGGCAGCTTGATTTTATAAGAAAACCATAGATGATAGCGATGGGCAATACATATAGTCAATCATGAACAACCTTCAGTATCTTTTTCACTTCACGCAAACACACATTCGCTGACAACCTTGCAGTCACTGAAAATTATCTGAGCACCGGAGGAGAACCAGTCTCTGAGCTTATTCACTACCAGCTCTTGTTCATCTTCATTTTCTAGAAATTTATGTTTTACTAACGCTGTACTGGTTGATTCGCATTCCTTACAGCACTTGTCTTTCAAAACAACCGACTGTAAGTTAGGAGCCCGGTCCATCACTGCTCCTATAAACAATAGATGTTCTTCTGATGCATTGAAACCAATGATTTCAAGTTCTTTCAGTGGCAAATGCTTCGGGTATGGAAACTCTGGCACTTCCCATGACGCATTACTTCTTTTAGCACACAACTTAGTTACTTTCTCGTCCTGCTCACATCGATGGTCATACACCTCAACTTCAAGTATCTCGAGGGATGGCGCAACTTCAAGAAGATTTGTTGTCCACAAAAGCCCAAATCCAACAAAGATATCATATATGCACAATTTCCTCAAATTGCTAAATGCTGAGGAAAGTTGATGTTTTTCAGGTAGTAGCCAAATCTTTTGCCCGAGGAAATCCAATGATAAAGTATTTATACATGCCGCGCCACATAGAAACTCACTTAACTTAAATTGTTCTTGATGAGGTGGCATGGCTGAAA



gi|34512053|gb|CF483184.1|CF483184 POL1_12_G03.b1_A002 Pollen Sorghum bicolor cDNA clone POL1_12_G03_A002 3', mRNA sequence

TGAAGCTGCCAGCATGATCTA
||||.||||||| | |||| |
ACTTTGACGGTCCTTCTAGCT

EST sequence (targeted fragment marked with yellow):
TTTTTTTTGAAACGGCCGGCAGGAGCTCTGCCCGGCATATATTAATAGAGAAGTAGAGCGCTACTACTTTTGCACATAATAAATCATAGTCAGCCTATCTTAACTACACAAGATGCTCTTCTGCATATAGAATATGGATGCTTTGTTCTTTGGATATACTTTACCCATTGGACGAAAATCCGGCCGAAATTCTACTCTGTACATCTTGGAAATCGATCTTCCTGGCAGTTTCAAGCAAAGCTAAAAGAAAAGGACATACAAGGACCAAGTCAAATTGCAAGACAATTGTAGAAATTTTACAGCATCGTAAAAACTAGAAACACTGCAAATGGATTACTATTTATTCAGAAAACACTATTTTCACATAACATATACCAAGCAACCTAATCTAGATTTGCAAGATGATGTTCATTCATGGCAATGCATGGAGCCAAGTGAAAAACTAATTATTTCAGATATTCTAGGAGAAGTTCCTTTGATGATTGGGTCAGTATACCAAACTGAATGACAGAGGACTATATATGAAGTGCTGGAACTAAACCTGGGAGTATGGACTAACCAAATCCAGGAGCACCACTGGATAGATATGGTAACCTGTATACATCAGCCTTTTCCAATTTCAGCATATATCTGCTTCAATGAAACGCTC