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mature miRNA: TGAAGCTGCCAGCATGATCTG
species: Helianthus annuus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|22311333|gb|BQ912554.1|BQ912554 QHA21A15.yg.ab1 QH_ABCDI sunflower RHA801 Helianthus annuus cDNA clone QHA21A15, mRNA sequenc

TGAAGCTGCCAGC-ATGATCTG
|||||||||||.| ||.|.|||
ACTTCGACGGTTGCTATTGGAC

EST sequence (targeted fragment marked with yellow):
GAATTCGGCCTTATGGCCTGTAGCCGGGATGTTGGTGGCGGCATCACCTGTACAGGTTATCGTTGGCAGCTTCATTGCAGAAGCAAAGAAACCAAAATCTAACGTCGGGCCATCACCAGTTGCACCACCAAACATGTTGAGTTTCGGTGGCGGCAGTGGCGGTGGAGGGGCGGTTCACGGTATAAGTCCTCCACCCGAAGGCGCATCAAGCGAATCTTCTGACGAAAGCGGGAGCAGCCCTCTTCATAGGATCCCGGGCTCCTACAACAACGCTACCCAACACCCACACCAGCACCAACACCAGCAGCAGCCGCAGCAGCAGCCGCCGCCACAGCCGCAGCACATGCAAAATCTTCCGATGTACTCGAACATGGGCTGGCCCAACTCCACCATGAACATGCTACCCAATTAACTACAAATACTAAATTTCCCTACAATAAATCTGACATCATGAGGATGATGGTTGTGGTGGCGGTGGCGGTGGGTGGTGGTTGTAGCGGCAAGTATACAGTTCCTTTCATATGCTAACGCGCATTATGCTTGAAAACAATCTTTTTATATTCCAGATTTCAAAATTTTTCTACCCTTCACTTTGCCTGCGGCTGTGGGTTAGTGTTGAAT