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mature miRNA: TGAAGCTGCCAGCATGATCTT
species: Jatropha curcas
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|311207475|gb|GW881255.1|GW881255 JC000819 Seed specific Normalized cDNA library from Jatropha curcas L. Jatropha curcas cDNA 

TGAAGCTGCCAGCATGATCTT
||||||||||| ||||||||
ACTTCGACGGT-GTACTAGAC

EST sequence (targeted fragment marked with yellow):
ATAACAAGCAAGAAAAGAACTACTAGTGAAATTTATAGCTATACATGTAGCTAGCTTGGGCTCTCTCAATAATAATCTACGACGAGACTGCCCATCTTCTTATCGTGTAACACTATCAGTTGAAGCTGCCAGCATGATCTTAACCTTCCTCCATAGTTGAGGAAAGATCAGATCATGTGGCAGCTTCACCCGTTGTGGTAGCACGAGAAACCCTAGTTTCACTCGAAACCCTTTTCTTTAAATGCTTAAATGGGCTGTTAGGGTTTTCTTTGTTCAATCCCTAGAAAAGGAGGGTTTTCAGTGACGGGGTTTTTGCGTCTCGGTGCAGTTCAACGATTTCTAGATCTTCCCTGGCATTTTTGGTGATTGAGCCTTGACATTCCTTAATCTGCCGTACTGCTTTTTG