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mature miRNA: TGAGAATCTTGATGATGCTGCAT
species: Lactuca sativa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|90521735|gb|DY983593.1|DY983593 CLSS6210.b1_C17.ab1 CLS(LMS) lettuce sativa Lactuca sativa cDNA clone CLSS6210, mRNA sequence

TGAGAATCTTGATGATGCTGCAT
|| ||||||||| |||||.|||.
ACACTTAGAACTTCTACGGCGTG

EST sequence (targeted fragment marked with yellow):
GCCGGGGATTCCATCCACCCTTCATCCTTCCACACACGACTAGGGTTTCTCTGCCTCAAACCCAATCATAACATGCCAACTGGGTTTTGCTCCTTCTTCATCTTCTTCCGTTTCTTCTGCACAACACCTTTCAATTTTCAAAACCACGAAAGTCATTGGTTACTGGTGCGGCATCTTCAAGATTCACATGCCAATATTCAACTCTAGATAAGTTTTTGGTTAATGGGTAATGAGAATCTTGATGATGCTGCATCAGAGATCAATCGACTACATTCCATTTACTACGGTTGCTCTGAATCTTATATAGAAAGGTAAACGTTATTAATTTCCGTACGTGTGTGTTTCTGTATGCTTTATCATATTATTAATTAAGCCCATCATGATTTTTCTTATGTCTATAGTCAATATCAAGTAGCCTTGGGTCGATAAAAAGTGCTATTTTTGTTCTTATTACCGATCTTTTGGTTAATTAAGTTTCTCTTGCTAATGTAATTGAATGGTGTTTTGCGGATATATGATGGGATCTTAATAGGCTCTAACAATATTGTCCAAGATAAAGAAACGGAAACAGAAACCGTATAACGCACATATATACACCTTTGCAAACTATCTCGTTCTCTGAAGAAGAACAACATCATTGAATTACAGGATCTGCTGATAGAAAGCGGTGAAGTCATCTAATTCGTACTTATTTATTTTAAAATGATATCTTCGTACTTATTTATTTAAAAATCATATCTGGGCGTCTTTTAAATTTTCATAATCTTCATGAATTAGTAGTTTATATGAGATCAAAAGATTTTTGTTTTTCTTTTGGTGCCTCAGTTACAGATCTTAGGGAGCAACGAAATTTCCTTTNCTTCTANGCTGCCCGTGTATGGAAAACCATCTTACACGTCAAAGCTGACGTGGCCGACTTTCCCACGCGTAAAGGGCATGT



gi|90518888|gb|DY980746.1|DY980746 CLSS2730.b1_C11.ab1 CLS(LMS) lettuce sativa Lactuca sativa cDNA clone CLSS2730, mRNA sequence

TGAGAATCTTGATGATGCTGCAT
|||||||.|||||||||||||
TCTCTTAGGACTACTACGACGTT

EST sequence (targeted fragment marked with yellow):
CGCGGGGGATTGTGGGAACAAGTTTATTTAGGCGGATTTGACACTGCCCTGGCTGCAGCTAGAGCCTATGATCGAGCTGCCATCAAGTTCAGGGGAATTGATGCGGATATCAACTTTCATATAAACGAATATGAGGAAGATATGGCACAGGTAAAGAATTTGTCAAAAGAAGAATTTATTCATATACTCCGTAGACAGAGCACAGGATTCTCACGAGGAAGTTCAAAATACCGAGGAGTAACCCTCCATAAATGTGGGAGATGGGAGGCTCGCATGGGTCAGCTTCTTGGAAAAAAGTATGTTTACCTTGGATTATTCGACAACGAAGTAGAAGCTGCAAGGTTGTTGTAATTTTATAATTGACAATTAACATGTTAACCCACCCATATGAACGAAGGCATCATTCAACAACATTTGCAATTTATATTGTCTGAATTCAGGGCTTATGACAAAGCTGCCATTAAATGTAACGGCAAGGAAGCTGTCACCAACTTTGAGCCACGCATCTATGGAGCGGATATAAGCTTGGAAGCCATGGATGAAGAAGCTAGTGGTCACAATCTTGATCTCAACCTCGGGATTTCTCCCACTAAAGATAGTCCAAGGGCAAATTTACAGGCACAGACACAAGCTCAAGCTCCGTTATTATGGCCTAGTAACATGTATTATGGTTTTACACCAAACAATGAGGAAAAAGCGATAGGAATGGCATCTGCGAATAATATTCAAGGGCTCTCAGCATGGCAAATGCAAATGTTGGGTGGCAATTATGGCATGGGTGTGACATTTCCAATTCCGGTTTCTTCTTCTATTGCAGCATCATCAGGATTCTCTTCTACTACTACTACTGTTTTCTCGAATGGGGGTAGTATCCAACAGCCAACTAACATGGCCAGGGGCACTTCTTCTTCGATATACTTTCAGTAACCAGATTGGTTGATAAAGCAAGCCCATGTGACTGCTTTGCTTGA