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mature miRNA: TGATGCTGCATCGGCAATAAA
species: Lactuca sativa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|83982925|gb|DW129034.1|DW129034 CLSX5832.b1_O17.ab1 CLS(XYZ) lettuce sativa Lactuca sativa cDNA clone CLSX5832, mRNA sequence

TGATGCTGCATCGGCAATAAA
.||| |||||||..|||| ||
GCTAAGACGTAGTTGTTAATT

EST sequence (targeted fragment marked with yellow):
TCACAATCTCGAAATTTCCCTTTCCTTTTTCGTTTTTTTGATCTTTAAGCATATGTATATAACTTATTGATTGCTTACTTCAGATATACTCTCAATGCAATATATATGAAGCAGGACAGAAGAAGGTGGCTTTTAAGTACCTCACTGAGAAGGCAGGTGACAAAGAGGAGGCATGCACAGGTTGCATTGTGTCTGAAGGTGATCTGTTTATGGCTGGAACTCAAGCTGGCTTATTGGCTCCCGATGCCGCAAGTGGCTTATTTCATCCAAGCCAACACAAGGCGAAATTTACTGTTGAACCTGCAACAATGGAACTTAAAGATGTGTTGAAGCGTTGTACAGGGTGGCAACAAGTTCCATGCCCAGCTGGCGAGTAATCGGTTGTGTATTCTTGGGTGAATATATATTATATTGAGAAATTTGGTTTTAATTGTTGATGCAGAATCGATTTTCCTGGTGGGTCCATCCCAATGACATGATTTTGAAGTGTATGAGTTAATCTATGTTGTATGAATCGAGTGTTTGAGAAGGGAGAAATCCGATTTATGATGCTAGGAACGATTATTTTCAAAAAAGAAATCAGATGCTTGTTGTTGATGGAAA