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mature miRNA: TGATTGAGACGAGTCCATATC
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|74156058|gb|DT624731.1|DT624731 EST1159066 Sequencing ESTs from loblolly pine embryos Pinus taeda cDNA clone PIMAI50 5' end,

TGATTGAGACGAGTCCATATC
|||||||| || |.| |||||
ACTAACTCGGCGCGGTTATAG

EST sequence (targeted fragment marked with yellow):
CACTTTCAAGGTAGGGGGTTCTGGCGCAGTGGCGGACGAGGTGCATGTAATAGTGGAGCAACTGCTCAAAGCAGCAGAGGCTGTGGAACTTGGCAACTTAGATCATGCTCAGGCGATATTGGCGCGGCTCAATCAGCATCTCTCCCCTCTCGGAAAGCCCCTTCAGCGGGCGGCTTTCTATTTCAAGGAGGCTCTCGCTTCTCGAATCCTCAACACCACCGCCACCAACACTGGCGGAGACAACCGGAACGCTACTGTGACTGGCACTGCAACTTCAAATATCTCTTCACCGCTGGACATGGTGCACAAGATAAGCGCATACAAGAGTTTCTCGGAGGCCTCTCCGCTGGCTCAATTCGCACATTTTACTGCCAACCAGGCATTCCTGGAAGCGCTGGAAGGTGCAGAGGCAATACATATCATAGATTTCGAAATCGGCTTGGGGGGTCAGTGGGCATCGTTTCTTCAAGAACTCGCTGTCAAACTCGGCGGCGCTCCGCCTGTCCGCATCACCGCGCTCGGCTCCTCGGCCTCCTCGTCCCTGGAGCTCCATCTTACGAGGGACAATCTATGCAATTTCGCCAAGCAGCTCAATGTTCCCTTCGAATTCGAGCTCCTCCATTTCGACCACATCGAGTCCCTGACGCTCCGCGAGCGGGAGGCCGTGGCGGTTAATCTTTCATTGTTGCCTTCTACTTTCAGTTCGCCGGACTCGATCTCGCGACTTCTCCGCCTCGTGAAGAACCTCGCGCCTAGGGCCGTGGTGGTGGTGGACGCCGAAACCACCGCCACCGCTGCCGCAGCCTCCGCCGCAACCACCTCCTCTGCCTCTTTTGTTCACCATTTCCTGGAAGCGCTGCAATTTTACTCTTTCCTGTTCGATTCCCTGGATGCCGTGAACATCAACATGGACGCCGTGCACAGATCGAGAAGTTCCTGCTAGCTCCGAGATCGACGCCACCATTTCCTCCGCGCGCTCGCCATGGAGACCCTGTTC



gi|66690032|gb|DR012526.1|DR012526 HEAT1_13_A01.g1_A029 Root at 37 C for 24 hr Pinus taeda cDNA clone HEAT1_13_A01_A029 5', mRNA

TGATTGAGACGAGTCCATATC
|||||||| || |.| |||||
ACTAACTCGGCGCGGTTATAG

EST sequence (targeted fragment marked with yellow):
TCGAGCCAAACTTCGCAGACCCGGGGGCCTTTGCTTTCAGTAACAATAATCTCAGCGATGTCATCGCTCCGCCACAGCACGCTTCTGTCCCGTCCAGTTTCCGCCCGTCTTATGCCACATTGCCTAACTTGCCCCAACAACAATTTATTCCGCCGCCTCCTCCACCTCCACCTCCTCCACCACCACCATTTGCCTACAATGGCCCCATGCCCACGCCCTTCGGTCCCAATGCCTATCATCCGGAGGTTCTTTTCTCCGGGCCAGCAGTCTACGGTTCGAATCCCGTGCAACACTTGGGACCAGATTTTCCCCGCTTTAACATGGCCGCGGATCCAAACCGGAACAATAATAATCTTTTGTTCGACATGCCACACCCGCCTCCTCCCAAACGTTTTGGACTTGAGCACCAGCTCTGGCAGCAGACATTCAAGCAGCAAGAATACATGAACATGATGAAACCCCAGCAGCAGCAAGAACTGCTGCAGTCTCTGCACAGGCGGCAGCAATTCCTCCACCAGGCACCGCCTCCGTCGCCGCACCACCAGATGAGGCAGAAGGCCGTTGTGAATCACACTTTCAAGGTAGGGGGTTCTGGCGCAGTGGCGGACGAGGTGCATGTAATAGTGGAGCAACTGCTCAAAGCAGCAGAGGCTGTGGAACTTGGCAACTTAGATCATGCTCAGGCGATATTGGCGCGGCTCAATCA