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mature miRNA: TGATTGAGCCGTGCCAATATC
species: Populus trichocarpa
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|52388076|gb|CV232487.1|CV232487 WS0197.B21_L22 PT-DX-N-A-10 Populus trichocarpa cDNA clone WS0197_L22 3', mRNA sequence

TGATTGAGCCGTGCCAATATC
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CCTAATTCGGCACGGTTTTGG

EST sequence (targeted fragment marked with yellow):
CTAAAAAATTAACCAGGGATCAAGTTGCTGTCCTGGAACAAGCTGTTGATGTCTACACTTATGAGTTCAGAAGGTTCATCACCTTCATCAGTGAGGTATTTGCAAATTCCCCTTTCTTTATTTCAGCAGAAGCTGCTGGTGCATTAGAAGCTAGAAACAATGATGACTACAAGGAGATAAATGTTCCAGCTGGGAAAACTCACGAGGTTTCATTGTCTGTGGAATCAGTGAACTCATACATTGCTTGGGATTTCAGTTTGATACAAGGCAAAATTAACATGGATATTGGATTTAGTGTGGAGTGTACAGACCCTACAGGGAAAAAAACTCTGATACTACCATATCGACGTTATGAGTCTGACCAAGGGAACTTCTCCACGTGCATATCTGGAAACTACAAGCTTATTTGGGATAATTCATATTCTGCATTTTTTAAAAAGGTCCTGCGTTACAAGGTGGACTGTATACCTCCGGTTGTTGAGCCAGTGCAATCTGCAGGTGAAATGGAAGAATAACTGTCGCCTCCTTAGCTTTAGCATTTGCGCCAAGATGCTGTCTCATGGTGATTTGGATCAAATTTATTTGTACTTGTTGCCTCTTCCTTTTTTTTTTTTTTTATCACATCCGATGAAAGAATGTTGCTTCTTGGTTTTGGCACGGCTTAATCCGCCGCTACACTAACTTGTTCTATGTTACCTGACAATCAGTATAACCCCATTCATCCAAAAAAAAAAAAAAAAAA