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mature miRNA: TGCACTGCCTCTTCCCTGGCT
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124693782|gb|EH788518.1|EH788518 CNSS28554.b1_D11.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS28554

TGCACTGCCTCTTCCCTGGCT
|||||| ||||||||||| |.
ACGTGAGGGAGAAGGGACGGG

EST sequence (targeted fragment marked with yellow):
GCGGGCATCCACTTACTTGGAATCCTCAAACCCGAATGCCATTGCCCCTTCAAATGGAAGAGGACCCTGTTTATGTGAACGCAAAACAATATCATGGGATTCTGAGGCGCAGACAGTCGAGAGCTAAAGCAGAATTGGAGAAGAAAGTGATTAAATCCAGAAAGCCATACCTTCATGAATCGCGACACCAACACGCTATGAAAAGAGCTCGGGGTTGTGGGGGTCGCTTCCTCAATACGAAAAAGCTCGGTAGTGACACAACCGCAAATTCCGCAGCACAAAAGGAGACAAGATCGATGTCAACGCAATCGTTCAACTCGGGTCATCCGTCCCCCAGAGATTCAGCCATCTATGAAGATGTGCTTAAAAGAAACAGTTGCTCAAATGGGAACAACAATGATCATCAACACAGTTTATCATCAACATACAACTTCCAGTTGATGGGCAGGGAAGAGGGAGTGCATTACTTCAAAAGGGACAAGGGCAATATGGTAAATCAAGTCCCAAAAAGGGATCCATATTACAAATGAGAAAAGGGAAGAGAGAGAGTATGTTCTCAGGCAATTCATTCTTGGCTTTGGTTACTTGTGTTCTCTTTGTGGGGTAGAGATATGGAAGGCTGGAATATGGTGAGAGGCCTTATTAACCAACATATCTNACAACCCTGTTTGTGCAATATGGAGTTGTATCATCTAGTANAAAAGTAAGTTGTACATTTTGGGATGTGGAATAGATGACCTACCTCATACTTGGAAGGTGTGATATTGTGTTTGTATGAGGTAGGATTACCTT



gi|124691355|gb|EH786091.1|EH786091 CNSS25345.b1_A01.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS25345

TGCACTGCCTCTTCCCTGGCT
|||||||||||| |||.||
TCGTGACGGAGAAAGGATCGT

EST sequence (targeted fragment marked with yellow):
AGAATATTAGCATCATTTTACACATAGTAACCAAAGAGATGCCGATGGTTGCATTCTTTGTTATCTTGATTCTATCCGTTGTGGCCTCGAGAACGTATGCGGTGCAGCATATCGTTGGTGACGGTGGTGGCTGGACCAATTCCGGAGATTATACTACATGGGCTTCCAGTAAGACATTCAATGTTGGCGACACACTTTTATTCAACTATGGTGGCAGCCATGGCGTGGACGTTCTATCGAAAGCCGACTACGACAATTGTGCCACGAGCAACGCCATCAACTCCTACACTGGAGGCACAACCACCATTAAGCTCACACAAGCCGGCCCAATGTACTTTGCTTGTCCATCATTTGGGCATTGTAGTTTAGGGATGAAAATGGCAATCAATGTGGTATCTAAAGCCACCACCTCACCTACCACAACCACCCCTCCCACTAGCGACGACAACCAATCACCACCATCGTCTACCACCCCATCGTCTACCACTCCATCTACTCCGGCAACTTCAAGTCCCACCGATGAAAGCAGTGGCACGAGCATGGTGATGGTGGCTTGGTTGTCTTTGATTTTGGCACCGGTGGCTGTGTTCATGTGCTAGGAAAGAGGCAGTGCTTGTACTTATGTTTTCTTTAATTAATTGTTAAGAGTTGTGTGATTTTGTATATTTAGTTTTTGTTTGGTGGAGGTGAAATGGAAAGTAAATAAAATGGAATGTAAGTTGATGCATTGGTGAATAGAAATATAAT